| Entry |
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| Name |
12alpha-hydroxysteroid dehydrogenase;
12alpha-hydroxy steroid dehydrogenase;
NAD+-dependent 12alpha-hydroxysteroid dehydrogenase;
NADP+-12alpha-hydroxysteroid dehydrogenase
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| Class |
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
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| Sysname |
12alpha-hydroxysteroid:NADP+ 12-oxidoreductase
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| Reaction(IUBMB) |
cholate + NADP+ = 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oate + NADPH + H+ [RN: R02793]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oate;
NADPH [CPD: C00005];
H+ [CPD: C00080]
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| Comment |
Catalyses the oxidation of the 12alpha-hydroxy group of bile acids, both in their free and conjugated form. Also acts on bile alcohols.
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| History |
EC 1.1.1.176 created 1978
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| Pathway |
| ec00121 | Secondary bile acid biosynthesis |
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| Reference |
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| Authors |
MacDonald IA, Mahony DE, Jellet JF, Meier CE. |
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| Title |
NAD-dependent 3alpha- and 12alpha-hydroxysteroid dehydrogenase activities from Eubacterium lentum ATCC no. 25559. |
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| Journal |
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| Reference |
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| Authors |
Mahony DE, Meier CE, Macdonald IA, Holdeman LV. |
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| Title |
Bile salt degradation by nonfermentative clostridia. |
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| Journal |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 1.1.1.176 |
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| LinkDB |
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