KEGG ENZYME: 1.1.1.297

ENZYME: 1.1.1.297

Entry

EC 1.1.1.297                Enzyme

Name

limonene-1,2-diol dehydrogenase;
NAD+-dependent limonene-1,2-diol dehydrogenase

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

menth-8-ene-1,2-diol:NAD+ oxidoreductase

Reaction(IUBMB)

menth-8-ene-1,2-diol + NAD+ = 1-hydroxymenth-8-en-2-one + NADH + H+ (general reaction) [RN:R08919];
(1) (1S,2S,4R)-menth-8-ene-1,2-diol + NAD+ = (1S,4R)-1-hydroxymenth-8-en-2-one + NADH + H+ [RN:R06399];
(2) (1R,2R,4S)-menth-8-ene-1,2-diol + NAD+ = (1R,4S)-1-hydroxymenth-8-en-2-one + NADH + H+ [RN:R09388]

Reaction(KEGG)

R06399 R08919 R09388

Substrate

menth-8-ene-1,2-diol [CPD:C18020];
NAD+ [CPD:C00003];
(1S,2S,4R)-menth-8-ene-1,2-diol [CPD:C07276];
(1R,2R,4S)-menth-8-ene-1,2-diol [CPD:C19082]

Product

1-hydroxymenth-8-en-2-one [CPD:C18019];
NADH [CPD:C00004];
H+ [CPD:C00080];
(1S,4R)-1-hydroxymenth-8-en-2-one [CPD:C11937];
(1R,4S)-1-hydroxymenth-8-en-2-one

Comment

While the enzyme from the Gram-positive bacterium Rhodococcus erythropolis DCL14 can use both (1S,2S,4R)- and (1R,2R,4S)-menth-8-ene-1,2-diol as substrate, activity is higher with (1S,2S,4R)-menth-8-ene-1,2-diol as substrate.

History

EC 1.1.1.297 created 2008

Pathway

ec00903

Limonene degradation

ec01120

Microbial metabolism in diverse environments

Reference

1 [PMID:10224006]

Authors

van der Werf MJ, Swarts HJ, de Bont JA

Title