KEGG ENZYME: 1.1.3.23

ENZYME: 1.1.3.23

Entry

EC 1.1.3.23                 Enzyme

Name

thiamine oxidase;
thiamin dehydrogenase;
thiamine dehydrogenase;
thiamin:oxygen 5-oxidoreductase

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor

Sysname

thiamine:oxygen 5-oxidoreductase

Reaction(IUBMB)

thiamine + 2 O2 + H2O = thiamine acetic acid + 2 H2O2 [RN:R02132]

Reaction(KEGG)

R02132;
(other) R00033 R00072

Substrate

thiamine [CPD:C00378];
O2 [CPD:C00007];
H2O [CPD:C00001]

Product

thiamine acetic acid [CPD:C02892];
H2O2 [CPD:C00027]

Comment

A flavoprotein (FAD). The product differs from thiamine in replacement of -CH2.CH2.OH by -CH2.COOH; the two-step oxidation proceeds without the release of the intermediate aldehyde from the enzyme.

History

EC 1.1.3.23 created 1984

Pathway

ec00730

Thiamine metabolism

ec01100

Metabolic pathways

Reference

1 [PMID:8076]

Authors

Edmondson DE, Kenney WC, Singer TP.

Title

Structural elucidation and properties of 8alpha-(N1-histidyl)riboflavin: the flavin component of thiamine dehydrogenase and beta-cyclopiazonate oxidocyclase.

Journal

Biochemistry 15:2937-45 (1976)
DOI:10.1021/bi00659a001

Reference

2 [PMID:2988447]

Authors

Gomez-Moreno C, Edmondson DE.

Title

Evidence for an aldehyde intermediate in the catalytic mechanism of thiamine oxidase.

Journal

Arch Biochem Biophys 239:46-52 (1985)
DOI:10.1016/0003-9861(85)90810-0

Reference

3 [PMID:4987737]

Authors

Neal RA.

Title

Bacterial metabolism of thiamine. 3. Metabolism of thiamine to 3-(2'-methyl-4'-amino-5'-pyrimidylmethyl)-4-methyl-thiazole-5-acetic acid (thiamine acetic acid) by a flavoprotein isolated from a soil microorganism.

Journal

J Biol Chem 245:2599-604 (1970)

Other DBs

ExplorEnz - The Enzyme Database:

1.1.3.23

IUBMB Enzyme Nomenclature:

1.1.3.23

ExPASy - ENZYME nomenclature database:

1.1.3.23

BRENDA, the Enzyme Database:

1.1.3.23

CAS:	96779-44-1

LinkDB

DBGET integrated database retrieval system