KEGG ENZYME: 1.14.13.20

ENZYME: 1.14.13.20

Entry

EC 1.14.13.20               Enzyme

Name

2,4-dichlorophenol 6-monooxygenase;
2,4-dichlorophenol hydroxylase;
2,4-dichlorophenol monooxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

2,4-dichlorophenol,NADPH:oxygen oxidoreductase (6-hydroxylating)

Reaction(IUBMB)

2,4-dichlorophenol + NADPH + H+ + O2 = 3,5-dichlorocatechol + NADP+ + H2O [RN:R03997]

Reaction(KEGG)

R03997;
(other) R05441

Substrate

2,4-dichlorophenol [CPD:C02625];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

3,5-dichlorocatechol [CPD:C02933];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

A flavoprotein (FAD). Also acts, more slowly, on 4-chlorophenol and 4-chloro-2-methylphenol; NADH can act instead of NADPH, but more slowly.

History

EC 1.14.13.20 created 1983

Pathway

ec00361

Chlorocyclohexane and chlorobenzene degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K10676

2,4-dichlorophenol 6-monooxygenase

Genes

PAKH:

B0X70_03075

PLUI:

CE143_03060

PCQ:	PcP3B5_21640(tfdB)

PNT:	G5B91_07365(hbpA)

PPUH:

B479_25850

PDR:	H681_09635

PSV:	PVLB_26087

MSHE:

MAALD49_03580

MANP:

EHN06_06635 EHN06_06685

ACB:

A1S_1079

ACAL:

BUM88_05190

ASEI:

I8T81_13610

ACD:	AOLE_13995

AEI:	AOY20_13805

ACUM:

C9E88_009150

ALW:	FOB21_04930

ACHI:

CDG60_06750

ASHA:

G8E00_06060

ABOU:

ACBO_09460

ACIU:

A3K93_08085

APOT:

MZO21_08345

ACIE:

KIP84_13070

OSG:	BST96_10705

MGEO:

CFI10_06255

CKS:	H9L41_22945

CNC:	CNE_BB2p00380(tfdB)

REU:	Reut_B4679 Reut_D6462 Reut_D6470

COX:	E0W60_36470 E0W60_36560 E0W60_36900

BCEN:

DM39_5592(tfdB)

BMJ:	BMULJ_00773

BMU:

Bmul_2460

BCT:

GEM_2096

BPSL:

WS57_14635

BANN:

JFN94_06885

BYI:	BYI23_F000530(mhqA) BYI23_F000610(mhqA)

BUZ:	AYM40_28715

PHS:	C2L64_52635

PCAF:

DSC91_003624

PACP:

FAZ97_19160

PPAI:

E1956_31265 E1956_41035 E1956_42495 E1956_45915

PPUL:

RO07_24965

TVL:	FAZ95_22945

BPT:

Bpet1331

AXY:	AXYL_03121

AIS:	BUW96_12410

APES:

FOC84_29900

AALN:

Q3V95_01245

PIG:	EGT29_10725

ADN:	Alide_3930

ADK:	Alide2_4275

DRG:	H9K76_13735

PAQA:

K9V56_003680

DAR:

Daro_0899

THK:	CCZ27_15525

MLN:	A9174_15455

MCI:	Mesci_5342

MCIC:

A4R28_00400

MAMO:

A6B35_10195

MESW:

A9K65_029190

MJR:	EB229_15655

MESO:

BSQ44_19310

RPOD:

E0E05_06010

NIY:	FQ775_05325

PLA:

Plav_0131

PECT:

BN1012_Phect175

RBS:	RHODOSMS8_01308(tfdB) RHODOSMS8_02317(tfdB) RHODOSMS8_03465(tfdB)

SIX:	BSY16_5886(tfdB)

EAK:	EKH55_4641

STEG:

QA637_22765

AGC:	BSY240_3583(tfdB)

AVV:	RvVAT039_pl00630

AVF:	RvVAR031_pl02700

AVI:

Avi_7439

RLE:	pRL120094(tfdB)

RLG:

Rleg_5534

RHL:	LPU83_3293(tfdB)

RGR:	FZ934_22095

RLN:	J0663_26265

RRG:	J3P73_27975

RROS:

D4A92_03065

RAW:	NE851_01970

RBW:	RLCC275e_25950

SHZ:

shn_17085

SOJ:	K6301_14535

SSUM:

Q9314_11240

ABAW:

D5400_18555

BRA:	BRADO2271(tfdB)

BRO:	BRAD285_6478(tfdB)

BRK:	CWS35_17625

BVZ:	BRAD3257_2215(tfdB)

BSEP:

HAP48_0030295

BQB:	J4P68_0031560

BBAN:

J4G43_002830

BCAN:

BcanWSM471_04095 BcanWSM471_18225

RBK:	E0H22_14530

LNE:	FZC33_07665

MSL:

Msil_1463

PHL:

KKY_3150

DEA:	FPZ08_02915

PLEO:

OHA_1_04031(tfdB)

MAAD:

AZF01_21740

MMED:

Mame_00353(tfdB_1)

MLUT:

JET14_22390

BRN:	D1F64_20720

RPON:

G3256_03730

PPIC:

PhaeoP14_02543

OAT:	OAN307_c31200

LEJ:	ETW24_15985

CEH:	CEW89_05510

SULZ:

C1J03_12300

PZH:	CX676_20335

PPAN:

ESD82_09880

PSTL:

JHW45_15635

PFEO:

E3U26_16715

RBG:	BG454_13025

TAW:	EI545_03210 EI545_15125

CACT:

HZ995_11470

NSM:	JO391_15080

AMYL:

QBD29_07650

NRE:	BES08_18240

SINA:

KNJ79_21045

SPHU:

SPPYR_1422

SWI:	Swit_2298 Swit_3045

SPHD:

HY78_10325 HY78_14035

RDI:	CMV14_05145

SPHF:

DM480_04910 DM480_13535

SJP:	SJA_C1-21170(tfdB)

SINB:

SIDU_04775

SPPH:

KFK14_07125 KFK14_10980 KFK14_23090

SCL:	sce6695(cadA)

EUZ:	DVS28_a4350

MMI:

MMAR_3384

MMAE:

MMARE11_32850

MDX:	BTO20_24030

MSIM:

MSIM_49230

MSAK:

MSAS_18810 MSAS_43450

MKU:	I2456_26965

MLJ:	MLAC_15310

MSPG:

F6B93_10655

MPAA:

MKK62_25015

MVA:

Mvan_4406

MYN:	MyAD_07550

MTHN:

4412656_01070(tfdB) 4412656_02644(tcmG)

MCHT:

MCHIJ_49160

MMAG:

MMAD_32310

MIJ:	MINS_17270

MARZ:

MARA_34500

MHEV:

MHEL_50940

MMUC:

C1S78_006365 C1S78_014655

MSEI:

MSEDJ_41060

MGRO:

FZ046_12670

CEF:

CE2219

CHN:	A605_00660 A605_12305

CMD:	B841_02870

CHM:	B842_00260 B842_11315

CDX:	CDES_03630(tfdB)

CCOE:

CETAM_00270(tfdB)

COK:	COCCU_00115(tfdB1) COCCU_00185(tfdB2)

CCYC:

SCMU_34840

CGF:	CGUA_01850(tfdB)

CSUE:

QP029_02345 QP029_06365

CBRE:

QP027_01750 QP027_10725

NSL:	BOX37_13460

NTP:	CRH09_29765

NWL:	NWFMUON74_39440 NWFMUON74_45630

NTC:	KHQ06_23985

RHA:	RHA1_ro00520(ohpB) RHA1_ro00538 RHA1_ro10313

REB:	XU06_29335

RQI:	C1M55_30810

ROP:

ROP_46190

ROA:	Pd630_LPD12046

RPY:	Y013_12685

RHB:

NY08_3120

RAV:	AAT18_09060 AAT18_09315

RFA:	A3L23_01332(tfdB)

RHS:	A3Q41_02037(tfdB)

RRZ:	CS378_24130

RHU:	A3Q40_02898(tfdB)

RHOD:

AOT96_34420

RKO:	JWS14_14265 JWS14_20530 JWS14_46965 JWS14_47000 JWS14_47920

ROZ:	CBI38_01165 CBI38_32790

RPSK:

JWS13_03800 JWS13_05395 JWS13_45120

GOC:	CXX93_14715

GAM:	GII34_02375

GOI:	LK459_00990

GSI:	P5P27_07205 P5P27_08345

TSD:	MTP03_45030

SRT:

Srot_2422

DIZ:	CT688_00820

SGRF:

SGFS_022110 SGFS_022650 SGFS_026090 SGFS_100090

SCB:	SCAB_12351 SCAB_14111 SCAB_23631

SVL:	Strvi_3710 Strvi_4586

SHY:

SHJG_8539

SHO:	SHJGH_8299

SDV:	BN159_1741

SCW:	TU94_32215

SXI:	SXIM_06260 SXIM_35670

STRC:

AA958_24210

SCX:	AS200_01245 AS200_38910

SLE:	sle_04010(sle_04010)

SGS:	AVL59_28985

STSI:

A4E84_33880

SGM:	GCM10017557_34850

SCAD:

DN051_01855 DN051_35330 DN051_39765 DN051_43520

SLS:	SLINC_1036

SNW:	BBN63_05670

SGE:	DWG14_00198(tfdB_1) DWG14_01104(tfdB_2)

SDX:	C4B68_17110

SCYA:

EJ357_04120 EJ357_37240

SFY:	GFH48_10085

SAQU:

EJC51_07500 EJC51_44160

SBY:	H7H31_31070

SPHV:

F9278_41170

SPAC:

B1H29_00665 B1H29_36395

SCHA:

CP983_03980

SCOE:

CP976_36295

STUI:

GCM10017668_59540

SCHF:

IPT68_02060

SHAR:

HUT13_00410

SFEU:

IM697_19750

SMOB:

J7W19_03210 J7W19_27645

SROI:

IAG44_02080 IAG44_37690

SMAO:

CAG99_11020 CAG99_26330

SINE:

KI385_36970

SLF:	JEQ17_02155 JEQ17_08035

SDEC:

L3078_39450

SAKB:

K1J60_04890

SCAE:

IHE65_05265

SANT:

QR300_06020

SARG:

HKX69_01025

SJN:	RI060_06610

SCYN:

N8I84_06530 N8I84_40540

SKG:	KJK29_04965

SCOA:

QU709_40790

STRZ:

OYE22_30335

KIS:	HUT16_21995

STRH:

GXP74_28295

ABRY:

NYE86_28185

AGLA:

OIE69_08690

MCAW:

F6J84_00360

MAZA:

NFX31_14545

MELY:

L2X98_27600 L2X98_30500

MVD:	AWU67_12435 AWU67_12440

FRP:	AX769_00705

AARC:

G127AT_14245

MDB:	OVN18_09695

SALD:

FVA74_01625

LRP:	MUN76_11660

SUBT:

KPL76_07445

ART:

Arth_1929

ACRY:

AC20117_12520

ARTP:

E5206_04650 E5206_13920

AJG:	KKR91_07500

ASUN:

KG104_03755

ASUF:

MNQ99_07130

AGEG:

MUG94_07235

ACH:

Achl_4564

PSEY:

GU243_16740

KPL:	KPaMU14_06545

NAE:	BHE16_06590

CIG:	E7744_15345

PAEY:

KUF55_08570

BCAU:

I6G59_06830 I6G59_09640 I6G59_09900

NANO:

G5V58_19740

NARO:

CFH99_24180

NAQU:

ENKNEFLB_03878(tfdB)

NRO:	K8W59_17650 K8W59_18520

NMAR:

HPC71_03210 HPC71_13685

PSIM:

KR76_25500

AER:	AERYTH_12990

AEF:	GEV26_15515

AWN:	NQV15_13620

KSL:	OG809_09400

NGV:	CDO52_16685

FRE:	Franean1_2986

NML:

Namu_0227

NAKA:

H7F38_04265

AMD:

AMED_5991

AMN:

RAM_30725

AMM:

AMES_5907

AMZ:

B737_5907

AMQ:	AMETH_1296

AMYC:

CU254_22230 CU254_25580

AACD:

LWP59_06385

APRT:

MUY14_05945

ARHD:

VSH64_17320 VSH64_18835

PSEA:

WY02_07855

PSEH:

XF36_06625

PHH:	AFB00_24145

PAUT:

Pdca_45420

PBRO:

HOP40_17930

PPEL:

H6H00_04965 H6H00_25965

KUT:	JJ691_01250 JJ691_13930

KBU:	Q4V64_48910

KPHY:

AOZ06_37215

AHM:	TL08_18505

UME:	RM788_03215

STP:	Strop_2185

MCRA:

ID554_06970

AFS:

AFR_25335

ACTL:

L3i22_033060

ASIC:

Q0Z83_037310

DVC:	Dvina_24335 Dvina_29130

DMAT:

Dmats_19960 Dmats_24370

EKE:	EK0264_03995 EK0264_08060 EK0264_11535

RMAR:

GBA65_09710

SBAE:

DSM104329_02808(tfdB)

ABAS:

ACPOL_6348 ACPOL_6587

» show all

Reference

1 [PMID:7075592]

Authors

Beadle CA, Smith AR.

Title

The purification and properties of 2,4-dichlorophenol hydroxylase from a strain of Acinetobacter species.

Journal

Eur J Biochem 123:323-32 (1982)
DOI:10.1111/j.1432-1033.1982.tb19771.x

Other DBs

ExplorEnz - The Enzyme Database:

1.14.13.20

IUBMB Enzyme Nomenclature:

1.14.13.20

ExPASy - ENZYME nomenclature database:

1.14.13.20

UM-BBD (Biocatalysis/Biodegradation Database):

1.14.13.20

BRENDA, the Enzyme Database:

1.14.13.20

CAS:	82047-82-3

LinkDB

DBGET integrated database retrieval system