KEGG   ENZYME: 1.14.13.38
Entry
EC 1.14.13.38               Enzyme                                 
Name
anhydrotetracycline 6-monooxygenase;
ATC oxygenase;
anhydrotetracycline oxygenase;
oxyS (gene name);
anhydrotetracycline monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
anhydrotetracycline,NADPH:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
anhydrotetracycline + NADPH + H+ + O2 = 12-dehydrotetracycline + NADP+ + H2O [RN:R04060]
Reaction(KEGG)
R04060
Substrate
anhydrotetracycline [CPD:C02811];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
12-dehydrotetracycline [CPD:C03206];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Streptomyces rimosus, participates in the biosynthesis of tetracycline antibiotics. It can also catalyse EC 1.14.13.234, 12-dehydrotetracycline 5-monooxygenase.
History
EC 1.14.13.38 created 1990, modified 2016
Pathway
ec00253  Tetracycline biosynthesis
ec01057  Biosynthesis of type II polyketide products
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K14256  anhydrotetracycline 6-monooxygenase / 5a,11a-dehydrotetracycline 5-monooxygenase
Genes
SVR: CP971_23960
SRIM: CP984_02460
SPLA: CP981_34445
SDRZ: NEH16_31700
SHAU: K9S39_12950
KAU: B6264_18535
NEX: NE857_24350
AGRA: AGRA3207_005105 AGRA3207_005106
AORI: SD37_34210
KUT: JJ691_15570
CROS: N8J89_05295
ACTL: L3i22_066710
DAUR: Daura_37815 Daura_37840
CCAZ: COUCH_15960
 » show all
Reference
1
  Authors
Behal, V., Hostalek, Z. and Vanek, Z.
  Title
Anhydrotetracycline oxygenase activity and biosynthesis of tetracyclines in streptomyces aureofaciens.
  Journal
Biotechnol Lett 1:177-182 (1979)
Reference
2  [PMID:2914874]
  Authors
Binnie C, Warren M, Butler MJ
  Title
Cloning and heterologous expression in Streptomyces lividans of Streptomyces rimosus genes involved in oxytetracycline biosynthesis.
  Journal
J Bacteriol 171:887-95 (1989)
DOI:10.1128/JB.171.2.887-895.1989
Reference
3  [PMID:3138982]
  Authors
Vancurova I, Volc J, Flieger M, Neuzil J, Novotna J, Vlach J, Behal V
  Title
Isolation of pure anhydrotetracycline oxygenase from Streptomyces aureofaciens.
  Journal
Biochem J 253:263-7 (1988)
DOI:10.1042/bj2530263
Reference
4  [PMID:23621493]
  Authors
Wang P, Bashiri G, Gao X, Sawaya MR, Tang Y
  Title
Uncovering the enzymes that catalyze the final steps in oxytetracycline biosynthesis.
  Journal
J Am Chem Soc 135:7138-41 (2013)
DOI:10.1021/ja403516u
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.38
IUBMB Enzyme Nomenclature: 1.14.13.38
ExPASy - ENZYME nomenclature database: 1.14.13.38
BRENDA, the Enzyme Database: 1.14.13.38
CAS: 70766-62-0
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