KEGG   ENZYME: 1.14.13.63
Entry
EC 1.14.13.63               Enzyme                                 
Name
3-hydroxyphenylacetate 6-hydroxylase;
3-hydroxyphenylacetate 6-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
3-hydroxyphenylacetate,NAD(P)H:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
3-hydroxyphenylacetate + NAD(P)H + H+ + O2 = 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O [RN:R02515 R02517]
Reaction(KEGG)
R02515 R02517
Substrate
3-hydroxyphenylacetate [CPD:C05593];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,5-dihydroxyphenylacetate [CPD:C00544];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
3-hydroxyphenylacetate 6-hydroxylase from Flavobacterium sp. is highly specific for 3-hydroxyphenylacetate and uses NADH and NADPH as electron donors with similar efficiency.
History
EC 1.14.13.63 created 1999
Pathway
ec00643  Styrene degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K10438  3-hydroxyphenylacetate 6-hydroxylase
Genes
SLB: AWJ20_2761 AWJ20_5196(ERG5)
MGR: MGG_03352
PPEI: PpBr36_06495
PGRI: PgNI_00700
FGR: FGSG_06447
FPU: FPSE_10099
FVN: FVRRES_11114
FVR: FVEG_04417
FOX: FOXG_07489 FOXG_10783
NHE: NECHADRAFT_53600 NECHADRAFT_87111
FFC: NCS54_01382600 NCS54_01416300
FKR: NCS57_01363400 NCS57_01398000
FMU: J7337_006456
MAW: MAC_02148
MAJ: MAA_00010 MAA_04437 MAA_06936
PCHM: VFPPC_09674
CMT: CCM_02612 CCM_07933
AMUS: LMH87_002934 LMH87_004841 LMH87_008787
PLJ: VFPFJ_04777
PTKZ: JDV02_000876 JDV02_002798
VAL: VDBG_09163
VDA: VDAG_07313
CFJ: CFIO01_02908 CFIO01_07804 CFIO01_13056
CLUP: CLUP02_09160 CLUP02_18154
CHIG: CH63R_07166 CH63R_10851 CH63R_13633 CH63R_13746
SAPO: SAPIO_CDS1218
ELA: UCREL1_2048 UCREL1_4076 UCREL1_7322
PFY: PFICI_01247 PFICI_01707
PSCO: LY89DRAFT_302569 LY89DRAFT_594882
ANI: ANIA_01397
AFM: AFUA_1G08800
ACT: ACLA_026760
NFI: NFIA_016920
AOR: AO090005001647
ANG: An08g00180
AFV: AFLA_004481
ALUC: AKAW2_30954S
ACHE: ACHE_31006S
APUU: APUU_10469A APUU_50302A APUU_80788S
PCS: N7525_011503
PDP: PDIP_41800
POU: POX_f08321
TRG: TRUGW13939_07309 TRUGW13939_08977
CIM: CIMG_13120(CIMG07204)
CPW: CPC735_035300
URE: UREG_05702
PBL: PAAG_11576(PAAG_02928)
ABE: ARB_02605
PNO: SNOG_13913
PTE: PTT_06974
BZE: COCCADRAFT_111657
BSC: COCSADRAFT_214700
BOR: COCMIDRAFT_41589
AALT: CC77DRAFT_1022627
ARAB: EKO05_0001002 EKO05_0009352
PFJ: MYCFIDRAFT_83850
CBET: CB0940_05131 CB0940_07072
NPA: UCRNP2_1523
 » show all
Reference
1  [PMID:1935954]
  Authors
Van Berkel WJ, Van Den Tweel WJ.
  Title
Purification and characterisation of 3-hydroxyphenylacetate 6-hydroxylase: a novel FAD-dependent monooxygenase from a Flavobacterium species.
  Journal
Eur J Biochem 201:585-92 (1991)
DOI:10.1111/j.1432-1033.1991.tb16318.x
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.63
IUBMB Enzyme Nomenclature: 1.14.13.63
ExPASy - ENZYME nomenclature database: 1.14.13.63
BRENDA, the Enzyme Database: 1.14.13.63
CAS: 114705-01-0
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