KEGG   ENZYME: 1.14.13.82
Entry
EC 1.14.13.82               Enzyme                                 
Name
vanillate monooxygenase;
4-hydroxy-3-methoxybenzoate demethylase;
vanillate demethylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
vanillate:oxygen oxidoreductase (demethylating)
Reaction(IUBMB)
vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde [RN:R05274]
Reaction(KEGG)
R05274
Substrate
vanillate [CPD:C06672];
O2 [CPD:C00007];
NADH [CPD:C00004];
H+ [CPD:C00080]
Product
3,4-dihydroxybenzoate [CPD:C00230];
NAD+ [CPD:C00003];
H2O [CPD:C00001];
formaldehyde [CPD:C00067]
Comment
Forms part of the vanillin degradation pathway in Arthrobacter sp.
History
EC 1.14.13.82 created 2000 as EC 1.2.3.12, transferred 2003 to EC 1.14.13.82
Pathway
ec00627  Aminobenzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K03862  vanillate monooxygenase
Genes
XCC: XCC0363(vanA)
XCB: XC_0375
XCA: xcc-b100_0389
XCP: XCR_4152
XCV: XCV0377
XAX: XACM_0351
XAC: XAC0363(vanA)
XCI: XCAW_00770(vanA)
XCT: J151_00485
XCJ: J158_00482
XCU: J159_00482
XCN: J169_00481
XCW: J162_00481
XCR: J163_00481
XCM: J164_00481
XCF: J172_00476
XFU: XFF4834R_chr03470
XAO: XAC29_01875
XOR: XOC_4549
XOZ: BE73_00785
XAL: XALC_3034(vanA)
XSA: SB85_14095
XVE: BJD12_14550
XPE: BJD13_15130
XHR: XJ27_17630
XGA: BI317_22060
XPH: XppCFBP6546_10005(XppCFBP6546P_10005)
XAN: AC801_01705
XAR: XB05_18445
XCZ: EBN15_01815
XTH: G4Q83_18280
XEU: XSP_000373
XHD: LMG31886_04100(tsaM1)
XPR: MUG10_08460
XDY: NYR95_02090
VOS: KNV97_08815
PAE: PA4904(vanA)
PAEV: N297_5074
PAEI: N296_5074
PAU: PA14_64800(vanA)
PAP: PSPA7_5628(vanA)
PAG: PLES_52901(vanA)
PAF: PAM18_5015(vanA)
PNC: NCGM2_0620(vanA)
PAEB: NCGM1900_5646(vanA)
PDK: PADK2_26010
PSG: G655_25860
PRP: M062_25870
PAEP: PA1S_26620
PAER: PA1R_gp2826
PAEM: U769_26885
PAEL: T223_27080
PAES: SCV20265_5564
PAEU: BN889_03196(vanA)
PAEG: AI22_07375
PAEC: M802_5072
PAEO: M801_4939
PRE: PCA10_51390(vanA)
PCQ: PcP3B5_56530(tsaM1_3)
PPAA: B7D75_25510
PPU: PP_3736(vanA)
PPF: Pput_2027
PPG: PputGB1_2170
PPW: PputW619_2045
PPB: PPUBIRD1_2026
PPI: YSA_09409
PPX: T1E_1717(vanA)
PPUT: L483_19445
PPUN: PP4_20850(vanA)
PFV: Psefu_4050
POR: APT59_07750
PALD: LU682_011510
PST: PSPTO_2904(vanA)
PSB: Psyr_2708
PSYR: N018_13725
PSP: PSPPH_2475(vanA)
PSAV: PSA3335_15475
PAMG: BKM19_016235
PAVL: BKM03_14475
PVD: CFBP1590__2812(vanA)
PCAB: JGS08_12860
PCOF: POR16_12565
PTRE: I9H09_12500
PSYI: MME58_14090
PAST: N015_13745
PFL: PFL_3471(vanA)
PPRC: PFLCHA0_c35050(vanA)
PPRO: PPC_3614
PFE: PSF113_2802
PFS: PFLU_3293
PPZ: H045_05725
PTV: AA957_00695
PCG: AXG94_01515
PVR: PverR02_12230
PMED: E3Z27_15085
PMUD: NCTC8068_02156(kshA_1)
PLUL: FOB45_22220
PBA: PSEBR_a2987
PBC: CD58_14310
PDR: H681_21940
PSV: PVLB_10605
PSK: U771_11805
PCH: EY04_12300
PCZ: PCL1606_35110
PCP: JM49_17570
PCHP: C4K32_2627
PFZ: AV641_10035
PSES: PSCI_1617
PSEM: TO66_13045
PPSY: AOC04_06905
PSOS: POS17_3599
PPSL: BJP27_22580
PUM: HGP31_09950
POJ: PtoMrB4_29730(vanA)
PPSH: G5J76_09050 G5J76_17005
PVK: EPZ47_14290
PEZ: HWQ56_13535
PBZ: GN234_02485
PMAO: PMYSY11_2124(vanA)
PASG: KSS96_11865
PALV: KSS97_15845
PZA: HU749_016095
PJU: L1P09_14540
PCAS: LOY40_15195
PKM: PZ739_11030
PTAI: ICN73_22530
PKK: QQ992_10500
PCUC: PSH97_15370
PTRL: OU419_25130
PPAE: LDL65_24295
PRHZ: CRX69_17870
PSJY: AA098_17195
PSKU: KUIN1_26820
PSTT: CH92_04375
ACB: A1S_1116
ABY: ABAYE2623(vanA)
ABN: AB57_1200
ABB: ABBFA_02396(tsaM1_1)
ABAB: BJAB0715_01306
ABAZ: P795_11745
ABAU: IX87_05265
ABAA: IX88_07830
ABAL: ABLAC_19570
ACC: BDGL_000396(vanA)
ANO: RR32_12590
ALC: OTEC02_13335
ACAL: BUM88_05435
ASEI: I8T81_13365
ACD: AOLE_13720
ACI: ACIAD0980(vanA)
AEI: AOY20_07280
ACW: A0J50_11960
AHL: AHTJS_03680
ASOL: BEN76_08720
AGU: AS4_34520(vanA)
AUG: URS_1853
ATN: FM020_02805
ATZ: M5E07_08245
AVIV: LF296_12220
ACIE: KIP84_12870
AAW: AVL56_08685
ALR: DS731_00575
PAT: Patl_0844
ALII: QR722_00590
CSA: Csal_0344
HEL: HELO_3940(vanA)
HAM: HALO0093
HMD: CTT34_17620
HSV: HNO53_19215
HPRO: LMS44_23210
HBH: E4T21_20970
HASO: B2G49_18375
HNP: SR894_21770
HTT: HZS52_05285
HCAM: I4484_18900
HSX: HNO51_18955
HTX: EKK97_21215
SAJS: QO259_02910
MMW: Mmwyl1_1296
MME: Marme_1172
MPC: Mar181_2609
MPRI: MP3633_2749
MARD: IBG28_05285
MFOI: JSY38_04510
MPRF: J8N69_01790
MRHI: KDW99_05575
MPON: MACH16_08910(vanA)
MARL: HH196_04930
MRZ: KDW95_00635
GSN: YC6258_03914
SALN: SALB1_1089
NBL: GJV52_04080
VIT: ADP71_28920
VST: LVJ81_00220
RSO: RSp0222(vanA)
RSE: F504_3661
RPU: CDC45_18725
RNC: GO999_23120
RSL: RPSI07_mp0173(vanA)
RSN: RSPO_m00210(vanA)
RSM: CMR15_mp10192(vanA)
RSY: RSUY_35550(tsaM1)
CBW: RR42_s0708 RR42_s2469
CCUP: BKK81_20160
CUP: BKK80_21645
CUU: BKK79_26810
BVI: Bcep1808_3498
BVE: AK36_3260
BUR: Bcep18194_B0420
BCM: Bcenmc03_5029
BCH: Bcen2424_5243
BCJ: BCAM2436(vanA)
BCEN: DM39_5402
BCEW: DM40_3099
BCEO: I35_6336(vanA)
BAM: Bamb_4591
BAC: BamMC406_5124
BMJ: BMULJ_05311(vanA)
BMU: Bmul_3214
BMK: DM80_4594
BMUL: NP80_3339
BCT: GEM_3253
BCED: DM42_5545
BCEP: APZ15_22685
BDL: AK34_5017
BPYR: ABD05_21690
BCON: NL30_16005
BDF: WI26_25000
BLAT: WK25_28075
BTEI: WS51_09170
BSEM: WJ12_32220
BPSL: WS57_01935
BMEC: WJ16_30710
BSTL: BBJ41_26115
BANN: JFN94_16415
BARI: NLX30_24955
BAEN: L3V59_27000
BGL: bglu_2g14550
BGU: KS03_4484
BUK: MYA_3124
BURK: DM992_23760
BGE: BC1002_3291
PSPW: BJG93_19170
PCAF: DSC91_002445
PACP: FAZ97_28965
PPK: U875_22470
PPNO: DA70_15150
PPNM: LV28_15530
PRB: X636_11695
PPUL: RO07_09575
PSPU: NA29_05490
PAPI: SG18_10075
PVE: UC34_01360
POX: MB84_05695
PFG: AB870_12615
PNR: AT302_09260
PAND: DRB87_01225
PFIB: PI93_001180
PCOM: NTU39_01585
TVL: FAZ95_34465
TCAR: U0034_04805
BOJ: CBF45_16195
PUD: G9Q38_04025 G9Q38_10650 G9Q38_11445 G9Q38_13355
CDN: BN940_08501
PHN: PAEH1_08445
PIG: EGT29_00385
KGY: EHF36_16230
NARM: N7E01_02480
RFR: Rfer_0280
RSB: RS694_12765
RHY: RD110_11775
RHF: EUB48_13000
RHOA: HZ993_17560
RHOB: HTY51_17265
POL: Bpro_3387
PNA: Pnap_2725
ACK: C380_22780
ACRA: BSY15_1230
ACIO: EAG14_01170
ARAD: KI609_21515
AMON: H9L24_11780
VEI: Veis_1368 Veis_1485
DAC: Daci_1352
DEL: DelCs14_5161
DTS: BI380_17925
DLA: I6G47_17780
VAP: Vapar_5693
VPE: Varpa_1917
VPD: VAPA_2c01950(vanA)
VAA: AX767_16425
VBO: CKY39_11895
VAM: C4F17_00155
CTT: CtCNB1_4192
CTES: O987_24985
CRJ: QMY55_20615
LIM: L103DPR2_01485(tsaM1)
LIH: L63ED372_00786(tsaM1_1)
HYR: BSY239_1252 BSY239_2692
HYB: Q5W_09005
HYL: LPB072_17960
HPSE: HPF_20485(tsaM2)
HYN: F9K07_04905
HTN: KI616_20740
HCZ: G9Q37_03425 G9Q37_13935
CBAA: SRAA_2261
CBAB: SMCB_0812
HGR: DW355_04310
STHM: IS481_07625
SNN: EWH46_19805
ATER: MW290_13485
LCH: Lcho_1335
HSE: Hsero_3992(vanA)
HSZ: ACP92_19930
HRB: Hrubri_3944(vanA)
HEE: hmeg3_01210
HHF: E2K99_20795
HFR: G5S34_21680
MASW: AM586_11615
MLIR: LPB04_14690
MALI: EYF70_16035
MUM: FCL38_02380
MFLA: GO485_27725
DZO: SR858_08485
TMJ: P0M04_23915
EHB: E7V67_023955
GLC: JQN73_01740
AZR: CJ010_15660
TCL: Tchl_3188
TAK: Tharo_2222
AGC: BSY240_3610
RROS: D4A92_02925
PDES: FE840_020065
BJA: blr2390(vanA)
BJU: BJ6T_74460
BJP: RN69_36110
BRA: BRADO1867
BBT: BBta_2184
BRS: S23_56180(vanA)
AOL: S58_56900
BRC: BCCGELA001_27935 BCCGELA001_31305
BRAD: BF49_2414
BIC: LMTR13_06900
BRO: BRAD285_5428(vanA)
BRK: CWS35_13720
BOT: CIT37_00690
BRQ: CIT40_06450
BGQ: X265_29230
BGZ: XH91_27175
BSYM: CIT39_05220
BBET: F8237_07970
BARH: WN72_12040
BVZ: BRAD3257_6671(vanA)
BEL: BE61_29140(vanA) BE61_81100(vanA)
BDG: LPJ38_33955
BSEP: HAP48_0007260
BQB: J4P68_0028645
BSEI: KMZ68_01225
BBAN: J4G43_013150
BCAN: BcanWSM471_10990
BAUT: QA635_10045 QA635_34875
BBRA: QA636_09125 QA636_36405
BGK: IC762_08185
BCOU: IC761_27405
BPAH: QA639_35115
BXN: I3J27_08065
BROS: QUH67_08280
BRAZ: LRP30_09785
RPA: TX73_018755
RPB: RPB_1908
RPT: Rpal_4139
RPX: Rpdx1_1730
RBK: E0H22_08395
TRB: HB776_01805
BOS: BSY19_2758
XDI: EZH22_08910
MNO: Mnod_7105
META: Y590_19195
MAQU: Maq22A_c10720(hcaE)
MEE: DA075_24625
MTEA: DK419_21100
MIND: mvi_02480(vanA)
MOC: BB934_00220
MTEZ: HPT29_013210
PEM: OF122_05180
DEI: C4375_09775
DSAL: K1X15_17485
DEVO: H4N61_07385
DOY: JI749_05685
MHAR: L1P08_06815
BRN: D1F64_22325
CCR: CC_2393
CCS: CCNA_02476(vanA)
CAK: Caul_1137
CSE: Cseg_2340
BRF: E4M01_02225
CON: TQ29_16445
PPRU: FDP22_15025
PGV: SL003B_2863
NPP: PP1Y_Mpl10434
NPN: JI59_20890
SINA: KNJ79_13900
SSAN: NX02_17555
SPAN: AWL63_04695
SKR: BRX40_12235
SECH: B18_11340
SPII: G7077_01585
SSUA: FPZ54_06190
SBIN: SBA_ch2_5410(vanA_3) SBA_ch2_5500(vanA_4)
SHAN: PPZ50_15715
SPHB: EP837_03492(vanA)
SPHY: CHN51_00900
BLAS: BSY18_3501
BFW: B5J99_08300
SPHS: ETR14_08005
RGI: RGI145_21085
ROS: CTJ15_00925
RMUC: FOB66_01760
ACIM: GT370_03550
AZL: AZL_b03640(vanA)
AZZ: DEW08_26970
AZS: E6C72_25000
NAO: Y958_28550
NCB: C0V82_19015
FER: FNB15_12425
THAI: IT893_15360
TTB: MACH01_30180(vanA)
GSO: PH603_12895
AVP: AVENP_2155(ligM)
PARH: I5S86_11620
MSEI: MSEDJ_44440
CGL: Cgl2383(Cgl2383)
CGB: cg2616(vanA)
CGU: WA5_2300(VanA)
CGT: cgR_2265
CGS: C624_11570
CGG: C629_11580
CGM: cgp_2616(vanA)
CGJ: AR0_11410
CGQ: CGLAR1_11260
CGX: SB89_11065
CEF: CE0634
CVA: CVAR_2696(vanA)
CHN: A605_00720
CCJZ: ccrud_10600
CSUR: N24_2454(vanA)
CCOE: CETAM_07705(tsaM1)
CCYC: SCMU_37970
CPOY: GP475_09050
BFV: C628_11540
RPY: Y013_13470
RRZ: CS378_17620
RHQ: IM25_04815
RRT: 4535765_04621
ROZ: CBI38_25030
GPO: GPOL_c13810
GOI: LK459_09205
SHY: SHJG_0203
MTS: MTES_2133
MIH: BJP65_08820
MOO: BWL13_01875(tsaM1)
AUM: AURMO_01281
SALC: C2138_12115
SALD: FVA74_12750
LRP: MUN76_08415
ARR: ARUE_c24110(vanA)
ACRY: AC20117_04925
ARTP: E5206_02400
ASUF: MNQ99_02465
ACAO: NF551_01670
AKO: N9A08_14415
GLU: F0M17_01970
GNC: QQS42_02090
KPL: KPaMU14_06260
KRS: EQG70_02565
NAE: BHE16_09630
PAEY: KUF55_17160
NAKA: H7F38_22045
GOB: Gobs_2826
SVI: Svir_26080
PBRO: HOP40_32900
ACTY: OG774_04295
JTL: M6D93_11230
MARM: YQ22_04915
MARB: CJ263_16380
 » show all
Reference
1  [PMID:3170489]
  Authors
Brunel F, Davison J
  Title
Cloning and sequencing of Pseudomonas genes encoding vanillate demethylase.
  Journal
J Bacteriol 170:4924-30 (1988)
DOI:10.1128/JB.170.10.4924-4930.1988
  Sequence
[ag:AAA26019]
Reference
2  [PMID:9098058]
  Authors
Priefert H, Rabenhorst J, Steinbuchel A
  Title
Molecular characterization of genes of Pseudomonas sp. strain HR199 involved in bioconversion of vanillin to protocatechuate.
  Journal
J Bacteriol 179:2595-607 (1997)
DOI:10.1128/JB.179.8.2595-2607.1997
  Sequence
[ag:CAA72287]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.82
IUBMB Enzyme Nomenclature: 1.14.13.82
ExPASy - ENZYME nomenclature database: 1.14.13.82
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.13.82
BRENDA, the Enzyme Database: 1.14.13.82
CAS: 39307-11-4
LinkDB

DBGET integrated database retrieval system