Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy

precorrin-3A,NADH:oxygen oxidoreductase (20-hydroxylating)

precorrin-3A + NADH + H+ + O2 = precorrin-3B + NAD+ + H2O [RN:R05217]

R05217
Reaction

precorrin 3A [CPD:C05772];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

An iron-sulfur protein. An oxygen atom from dioxygen is incorporated into the macrocycle at C-20. In the aerobic cobalamin biosythesis pathway, four enzymes are involved in the conversion of precorrin-3A to precorrin-6A. The first of the four steps is carried out by EC 1.14.13.83, precorrin-3B synthase (CobG), yielding precorrin-3B as the product. This is followed by three methylation reactions, which introduce a methyl group at C-17 (CobJ; EC 2.1.1.131), C-11 (CobM; EC 2.1.1.133) and C-1 (CobF; EC 2.1.1.152) of the macrocycle, giving rise to precorrin-4, precorrin-5 and precorrin-6A, respectively.

EC 1.14.13.83 created 2004