KEGG ENZYME: 1.14.15.4

ENZYME: 1.14.15.4

Entry

EC 1.14.15.4                Enzyme

Name

steroid 11beta-monooxygenase;
steroid 11beta-hydroxylase;
steroid 11beta/18-hydroxylase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

steroid,reduced-adrenodoxin:oxygen oxidoreductase (11beta-hydroxylating)

Reaction(IUBMB)

a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O [RN:R02726]

Reaction(KEGG)

R02726 > R02725;
(other) R02218 R02843 R03262 R03263 R03329 R03851 R04850 R08949

Substrate

steroid [CPD:C00377];
reduced adrenodoxin [CPD:C00662];
O2 [CPD:C00007];
H+ [CPD:C00080]

Product

11beta-hydroxysteroid [CPD:C01058];
oxidized adrenodoxin [CPD:C00667];
H2O [CPD:C00001]

Comment

A heme-thiolate protein (P-450). Also hydroxylates steroids at the 18-position, and converts 18-hydroxycorticosterone into aldosterone.

History

EC 1.14.15.4 created 1961 as EC 1.99.1.7, transferred 1965 to EC 1.14.1.6, transferred 1972 to EC 1.14.15.4, modified 1989, modified 2014

Pathway

ec00140

Steroid hormone biosynthesis

ec01100

Metabolic pathways

Orthology

K00497

steroid 11beta-monooxygenase

K07433

steroid 11beta-monooxygenase / corticosterone 18-monooxygenase

Genes

HSA:	1584(CYP11B1) 1585(CYP11B2)

PTR:	464437(CYP11B1) 745937(CYP11B2)

PPS:	100971238(CYP11B2) 100971586(CYP11B1)

GGO:	101142418 115930159

PON:	100459910 100461139

NLE:	100605657 115830633

HMH:	116465197 116465198

MCC:	695793(CYP11B1) 695914(CYP11B2)

MCF:	102136659(CYP11B1) 102137301(CYP11B2)

MTHB:

126961020 126961026 126961618

MNI:	105483796 105488428 105488429

CSAB:

103237546(CYP11B1) 103237547(CYP11B2)

CATY:

105581188 105581294

PANU:

101002490 101002842 116276421

TGE:	112630213 112630247

MLEU:

105536287 105536288

RRO:	104679773 104679775

RBB:	108536535 108536550

TFN:	117078861 117079172 117079174

PTEH:

111535654

CANG:

105509012 105509013

CJC:	100415304(CYP11B2) 100415667(CYP11B1)

SBQ:

101048076

CIMI:

108291253(CYP11B1) 108291254(CYP11B2)

CSYR:

103276447 103276450

MMUR:

105867373 105867374

LCAT:

123644516 123644520

PCOQ:

105813307 105813360

OGA:	100948075 100948396 100958146

MMU:	110115(Cyp11b1) 13072(Cyp11b2)

MCAL:

110310257 110310258

MPAH:

110334511 110335023

RNO:	120093683(Cyp11b2l1) 24294(Cyp11b2) 353498(Cyp11b3) 500892(Cyp11b1)

MCOC:

116073894 116082436

ANU:	117719036 117719092

MUN:	110544545 110562757

CGE:	100771648 107977023

MAUA:

101823339 101823606

PROB:

127225603 127225649 127225650

PLEU:

114684742 114684743

MORG:

121461414 121461416

MFOT:

126503589 126503590

AAMP:

119823440 119823447

NGI:	103748431 103748432

HGL:	101700701 101701311

CPOC:

100379213(Cyp11b2) 100727186 111755182

CCAN:

109681807 109681816

DORD:

105992320 106002066

DSP:	122125332 122125344

PLOP:

125360501 125361022

NCAR:

124991075 124991346

MMMA:

107142805 107143071

OCU:	100328543(CYP11B2) 100342863

OPI:

101518686

TUP:	102471986 102492986

GVR:	103599088 103604864 103604866

CFA:	482071(CYP11B2)

CLUD:

112662131

VVP:

112911200

VLG:

121499022

NPO:

129502591

AML:

100478197

UMR:

103680551

UAR:	123779294 123796461

ELK:

111146729

LLV:	125098158 125099244

MNP:

132013308

MLK:	131827767 131827768

NVS:	122905498 122905499

ORO:

101364874

EJU:	114204069 114204070

ZCA:	113911085 118356903

MLX:

118015252

NSU:

110578976

LWW:

102732818

FCA:	101097790(CYP11B1)

PYU:	121024468 121043245

PCOO:

112859405

PBG:	122496110 122496111

PVIV:

125156496

LRUF:

124507499 124507515

PTG:

102972244

PPAD:

109258417

PUC:

125924912

AJU:	106989683 128312793

HHV:

120227687

BTA:	282422(CYP11B1) 787628

BOM:	102272939 102280195

BIU:	109568177 109568372 109568377

BBUB:

112577757 112579063

BBIS:

104997957

CHX:	102188238 102188517

OAS:	101102377 767576(CYP11B1)

BTAX:

128059464 128059476

ODA:

120859420

CCAD:

122451091

MBEZ:

129560226

SSC:

110260194

CFR:

102517728

CBAI:

105077899

CDK:

105106427

VPC:

102532708

BACU:

103009357

BMUS:

118883237

LVE:

103089121

OOR:

101286768

DLE:

111165107

PCAD:

102988484

PSIU:

116742421

NASI:

112397123

ECB:	100063612 100063645

EPZ:

103543718

EAI:	106827552 123275604

MYD:

102752229

MMYO:

118670722

PKL:

118718613

EFUS:

103302673

MNA:

107542744

DRO:

112315587

SHON:

118988945

AJM:

119064760

PDIC:

114514446

PHAS:

123833011

MMF:

118643316

PPAM:

129084099

HAI:

109390820

RFQ:

117033759

PALE:

102881458

PGIG:

120588772

PVP:	105302023 105310134

RAY:

107507877

MJV:

108400067

TOD:

119232024

SARA:

101544036

LAV:

100654178

TMU:

101354125

ETF:

101664498

DNM:

101424711

MDO:

100032885

GAS:

123255806

SHR:

100930767

AFZ:

127544520

PCW:

110214194

OAA:

100082065

TGU:

116807520

LSR:

110479458

PHI:

102113159

FPG:

101918505

FCH:

102046275

AROW:

112976664

ASN:

102375758

AMJ:	102575737(CYP11B1)

CPOO:

109310774

CMY:

102933677

CCAY:

125630881

DCC:

119851557

CPIC:

101950678

TST:

117873891

CABI:

116833743

MRV:

120396417

ACS:

100560890

ASAO:

132773556

PVT:

110084842

SUND:

121929727

PBI:

103058342

PMUR:

107300041

CTIG:

120298053

TSR:

106545562

PGUT:

117666942

APRI:

131196363

VKO:

123035256

PMUA:

114600140

PRAF:

128416955

ZVI:

118089700

HCG:

128322106

GJA:

107116366

STOW:

125425154

EMC:

129333751

XLA:

108718624

XTR:

101731070

RTEM:

120921857

BBUF:

121001585

BGAR:

122938238

MUO:

115481511

GSH:

117353600

DRE:	791124(cyp11c1)

SRX:

107745157

SANH:

107698185

SGH:

107570796

CCAR:

109110857

CAUA:

113059430

CGIB:

128030261

PTET:

122357920

LROH:

127178203

OMC:

131521331

PPRM:

120491104

RKG:

130103912

MAMB:

125243223

CIDE:

127497506

MASI:

127453357

TDW:

130432709

MANU:

129448943

IPU:

108263488

PHYP:

113525473

SMEO:

124384380

TFD:

113647799

TVC:

132846065

AMEX:

103026652

CMAO:

118813895

EEE:

113574779

CHAR:

105912980

TRU:

101075863

TFS:

130532956

TNG:	GSTEN00015374G001

LCO:

104920541

NCC:

104944920

TBEN:

117480465

CGOB:

115021232

PGEO:

117460921

GACU:

117541005

EMAC:

134861593

ELY:

117265363

EFO:

125893014

PLEP:

121945728

SLUC:

116049596

ECRA:

117946724

ESP:

116691100

PFLV:

114556971

GAT:

120810783

PPUG:

119198098

AFB:

129110285

CLUM:

117745803

PSWI:

130213196

MSAM:

119897544

SCHU:

122881607

CUD:

121514148

ALAT:

119006071

MZE:

101463871

ONL:	100533461(cyp11b)

OAU:

116320943

OLA:	100125822(cyp11b)

OML:

112143274

CSAI:

133432964

XMA:

102236274

XCO:

114139300

XHE:

116715552

PRET:

103478110

PFOR:

103136813

PLAI:

106957061

PMEI:

106922010

GAF:

122830567

PPRL:

129371107

CVG:

107088793

CTUL:

119783082

GMU:

124865602

NFU:

107379700

KMR:

108235361

ALIM:

106515094

NWH:

119410002

AOCE:

111567446 111575932

MCEP:

125015727

CSEM:

103388284

POV:

109633048

SSEN:

122774868

HHIP:

117777191

HSP:

118113534

SMAU:

118299793

LCF:

108885901

SDU:

111230014

SLAL:

111652162

XGL:

120784322

HCQ:

109522775

SSCV:

125975714

SBIA:

133500663

PEE:

133401343

PTAO:

133479217

BPEC:

110163163

MALB:

109970182

BSPL:

114842551

SJO:

128366469

SASA:

100136945

OTW:

121840636

OMY:	100135891(cyp11b) 110534298

OGO:	123998350 124004644

ONE:

115122985

OKI:

109881262

OKE:	118373241 127915287

SALP:

112074060

SNH:

120047273

CCLU:

121543144 123481512

ELS:

105018961

SFM:

108925533

PKI:

111853322

AANG:

118210794

LOC:

102683152

PSPA:

121306922

ARUT:

117395226 117969833

PSEX:

120518870

LCM:

102345423

» show all

Reference

1 [PMID:13276417]

Authors

GRANT JK, BROWNIE AC.

Title

The role of fumarate and TPN in steroid enzymic 11beta-hydroxylation.

Journal

Biochim Biophys Acta 18:433-4 (1955)
DOI:10.1016/0006-3002(55)90111-6

Reference

2 [PMID:13211659]

Authors

HAYANO M, DORFMAN RI.

Title

On the mechanism of the C11 beta-hydroxylation of steroids.

Journal

J Biol Chem 211:227-35 (1954)

Reference

3 [PMID:13426185]

Authors

TOMKINS GM, MICHAEL PJ, CURRAN JF.

Title

Studies on the nature of steroid 11-beta hydroxylation.

Journal

Biochim Biophys Acta 23:655-6 (1957)
DOI:10.1016/0006-3002(57)90396-7

Reference

4 [PMID:3485096]

Authors

Yanagibashi K, Haniu M, Shively JE, Shen WH, Hall P

Title

The synthesis of aldosterone by the adrenal cortex. Two zones (fasciculata and glomerulosa) possess one enzyme for 11 beta-, 18-hydroxylation, and aldehyde synthesis.

Journal

J Biol Chem 261:3556-62 (1986)

Reference

5 [PMID:4967077]

Authors

Zuidweg MH.

Title

Hydroxylation of Reichstein's compound S with cell-free preparations from Curvularia lunata.

Journal

Biochim Biophys Acta 152:144-58 (1968)
DOI:10.1016/0005-2760(68)90016-7

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