KEGG   ENZYME: 1.17.1.5
Entry
EC 1.17.1.5                 Enzyme                                 
Name
nicotinate dehydrogenase;
nicotinic acid hydroxylase;
nicotinate hydroxylase
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With NAD+ or NADP+ as acceptor
Sysname
nicotinate:NADP+ 6-oxidoreductase (hydroxylating)
Reaction(IUBMB)
nicotinate + H2O + NADP+ = 6-hydroxynicotinate + NADPH + H+ [RN:R01720]
Reaction(KEGG)
R01720
Substrate
nicotinate [CPD:C00253];
H2O [CPD:C00001];
NADP+ [CPD:C00006]
Product
6-hydroxynicotinate [CPD:C01020];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
A flavoprotein containing non-heme iron. The enzyme is capable of acting on a variety of nicotinate analogues to varying degrees, including pyrazine-2-carboxylate, pyrazine 2,3-dicarboxylate, trigonelline and 6-methylnicotinate. The enzyme from Clostridium barkeri also possesses a catalytically essential, labile selenium that can be removed by reaction with cyanide.
History
EC 1.17.1.5 created 1972 as EC 1.5.1.13, transferred 2004 to EC 1.17.1.5
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K20445  nicotinate dehydrogenase FAD-subunit
K20446  nicotinate dehydrogenase small FeS subunit
K20447  nicotinate dehydrogenase large molybdopterin subunit
K20448  nicotinate dehydrogenase medium molybdopterin subunit
Genes
SOFNCTC11214_04501(ndhM)
HAXIHAALTHF_35790n
UPIEJG51_001725
BVZBRAD3257_6051
MALUKU6B_09420 KU6B_24970
VSUP3M64_05615
DTIDesti_0416 Desti_0691
CPSSM5V91_09065
CPATCLPA_c02860(ndhF1) CLPA_c02870(ndhS1) CLPA_c02880(ndhL)
CPAECPAST_c02860(ndhF1) CPAST_c02870(ndhS1) CPAST_c02880(ndhL)
CINTHZF06_02100
CALDPWK10_12180 PWK10_12185
VFAMM35RIKEN_03290
ACOLK5I23_11315 K5I23_11320 K5I23_11325
PETRQKW49_07170
DORDesor_0445 Desor_0446 Desor_1381 Desor_1382
DAIDesaci_2061 Desaci_2062 Desaci_2063
DMIDesmer_1309 Desmer_1310
FWADCMF_22605 DCMF_25575
AACXDEACI_1213 DEACI_1473 DEACI_1474 DEACI_1596 DEACI_3801 DEACI_3802
ACAEHYG86_14455 HYG86_14460
PROMQO263_02190 QO263_18065 QO263_18070 QO263_18075 QO263_18080
BLIQINP51_10785
BLARLC508_08175
BCOCBLCOC_22520(ndhS_3)
ETMCE91St48_21740
AMTAmet_4569
AOEClos_0375 Clos_0377
TEBT8CH_1929(xdhC)
CHYCHY_0690(coxM)
MTAMoth_1225 Moth_1226
MTHOMOTHE_c12030(ndhM) MOTHE_c12040(ndhL)
MTHZMOTHA_c12900(ndhM) MOTHA_c12910(ndhL)
TEPTepRe1_0411 TepRe1_0412 TepRe1_0413
TAETepiRe1_0458 TepiRe1_0459 TepiRe1_0460(ndhM)
NTHNther_2150 Nther_2151 Nther_2152 Nther_2153
HHLHalha_1050
TISP3962_00500 P3962_08805 P3962_08810 P3962_08820
TIZRBU61_01420 RBU61_01430 RBU61_01435
ETTAZF37_06400
XCLG4Z02_01140
TBKHF295_00970
PFLAPflav_009980 Pflav_036150 Pflav_072710 Pflav_072730
IPOIlyop_0111 Ilyop_0112 Ilyop_0113 Ilyop_0114
TFEJYK00_05500
PMOPmob_1740 Pmob_1741
 » show all
Reference
1  [PMID:4388026]
  Authors
Holcenberg JS, Stadtman ER.
  Title
Nicotinic acid metabolism. 3. Purification and properties of a nicotinic acid hydroxylase.
  Journal
J Biol Chem 244:1194-203 (1969)
Reference
2  [PMID:8555176]
  Authors
Gladyshev VN, Khangulov SV, Stadtman TC
  Title
Properties of the selenium- and molybdenum-containing nicotinic acid hydroxylase from Clostridium barkeri.
  Journal
Biochemistry 35:212-23 (1996)
DOI:10.1021/bi951793i
  Sequence
Reference
3  [PMID:8278371]
  Authors
Gladyshev VN, Khangulov SV, Stadtman TC.
  Title
Nicotinic acid hydroxylase from Clostridium barkeri: electron paramagnetic resonance studies show that selenium is coordinated with molybdenum in the catalytically active selenium-dependent enzyme.
  Journal
Proc Natl Acad Sci U S A 91:232-6 (1994)
DOI:10.1073/pnas.91.1.232
Reference
4  [PMID:6838209]
  Authors
Dilworth GL.
  Title
Occurrence of molybdenum in the nicotinic acid hydroxylase from Clostridium barkeri.
  Journal
Arch Biochem Biophys 221:565-9 (1983)
DOI:10.1016/0003-9861(83)90176-5
Reference
5  [PMID:7181513]
  Authors
Dilworth GL.
  Title
Properties of the selenium-containing moiety of nicotinic acid hydroxylase from Clostridium barkeri.
  Journal
Arch Biochem Biophys 219:30-8 (1982)
DOI:10.1016/0003-9861(82)90130-8
Reference
6
  Authors
Nagel, M. and Andreesen, J.R.
  Title
Purification and characterization of the molybdoenzymes nicotinate dehydrogenase and 6-hydroxynicotinate dehydrogenase from Bacillus niacini.
  Journal
Arch Microbiol 154:605-613 (1990)
Other DBs
ExplorEnz - The Enzyme Database: 1.17.1.5
IUBMB Enzyme Nomenclature: 1.17.1.5
ExPASy - ENZYME nomenclature database: 1.17.1.5
BRENDA, the Enzyme Database: 1.17.1.5
CAS: 9059-03-4
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