KEGG ENZYME: 2.1.1.127

ENZYME: 2.1.1.127

Entry

EC 2.1.1.127                Enzyme

Name

[ribulose-bisphosphate carboxylase]-lysine N-methyltransferase;
rubisco methyltransferase;
ribulose-bisphosphate-carboxylase/oxygenase N-methyltransferase;
ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilonN-methyltransferase;
S-adenosyl-L-methionine:[3-phospho-D-glycerate-carboxy-lyase (dimerizing)]-lysine 6-N-methyltransferase;
RuBisCO methyltransferase;
RuBisCO LSMT

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:[3-phospho-D-glycerate-carboxy-lyase (dimerizing)]-lysine N6-methyltransferase

Reaction(IUBMB)

3 S-adenosyl-L-methionine + [ribulose-1,5-bisphosphate carboxylase]-L-lysine = 3 S-adenosyl-L-homocysteine + [ribulose-1,5-bisphosphate carboxylase]-N6,N6,N6-trimethyl-L-lysine [RN:R05179]

Reaction(KEGG)

R05179

Substrate

S-adenosyl-L-methionine [CPD:C00019];
[ribulose-1,5-bisphosphate carboxylase]-L-lysine [CPD:C06403]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
[ribulose-1,5-bisphosphate carboxylase]-N6,N6,N6-trimethyl-L-lysine [CPD:C06404]

Comment

The enzyme catalyses three successive methylations of Lys-14 in the large subunits of hexadecameric higher plant ribulose-bisphosphate-carboxylase (EC 4.1.1.39). Only the three methylated form is observed [3]. The enzyme from pea (Pisum sativum) also three-methylates a specific lysine in the chloroplastic isoforms of fructose-bisphosphate aldolase (EC 4.1.2.13) [5].

History

EC 2.1.1.127 created 1999, modified 2012

Orthology

K00592

[ribulose-bisphosphate carboxylase]/[fructose-bisphosphate aldolase]-lysine N-methyltransferase

Genes

ATEN:

116294947

PDAM:

113668217

ATH:	AT1G14030(LSMT-L)

ALY:

9326099

CRB:

17896815

CSAT:

104740061 104755697 104772851

EUS:	EUTSA_v10007614mg

BRP:

103843008

BNA:	106369122 106415812

BOE:

106307554

RSZ:

108806159

THJ:

104801808

CPAP:

110814551

CIT:

102629962

CIC:	CICLE_v10031325mg

PVY:

116120564

MINC:

123213207

TCC:

18610345

GRA:

105794210

GHI:	107887371 107910114

GAB:

108483107

HSYR:

120169338 120210702

DZI:

111318315

EGR:	104422322 104434604 104453926 104453927 120293321

GMX:	100801721 100818113

GSJ:	114403778 114419860

PVU:	PHAVU_002G169400g PHAVU_005G037700g

VRA:

106766684

VAR:

108345304

VUN:

114177883

VUM:	124826011 124828742

CCAJ:

109790121

APRC:

113858026

MTR:

25492966

TPRA:

123907359

CAM:

101490590

PSAT:

127108354

VVO:

131602457

LJA:	Lj4g3v0684250.2(Lj4g3v0684250.2)

ADU:

107481199

AIP:

107631855

AHF:	112734120 112791958

LANG:

109339197 109349547

PCIN:

129322346

QSA:	O6P43_025500

FVE:

101298121

RCN:

112197725

PPER:

18793936

PMUM:

103321538

PAVI:

110751405

PDUL:

117633227

MDM:

103425473

MSYL:

126607823

PXB:

103947201

ZJU:

107428930

MNT:

21398778

CSAV:

115700794

CSV:

101205332

CMO:

103485765

BHJ:

120080317

MCHA:

111023237

CMAX:

111471356 111471861

CMOS:

111450646 111454704

CPEP:

111799237 111805617 111805618

RCU:

8281847

JCU:

105630482

HBR:	110651139 110652291

MESC:

110625317

POP:

7498187

PEU:

105121014

PALZ:

118062350

JRE:

108992916

CILL:

122311345

CAVE:

132179908

QSU:

112002850

QLO:

115978779

TWL:

120001376

VVI:

100243771

VRI:

117912770

SLY:

101259632

SPEN:

107020229 107020231

SOT:	102587278 102587919

SSTN:

125866777 125867676

SDUL:

129880149

CANN:

107847854 107848269

LBB:	132642039 132642040

NTA:	107778201(RBCMT) 107809813

NSY:

104226426

NTO:

104089653

NAU:

109214572

INI:

109161120

ITR:

116004215

SIND:

105159997

OEU:

111398907

EGT:

105967526

SSPL:

121798072

SMIL:

130995799

SHIS:

125205214

APAN:

127261740

HAN:

110911425

ECAD:

122578954

LSV:

111879053

CCAV:

112527798

DCR:

108202653

CSIN:

114264208 114306448

RVL:

131309937

AEW:

130779423

BVG:

104897864

SOE:

110785063

CQI:	110706360 110712993

ATRI:

130809122 130820119

MOF:	131143755 131156313

NNU:

104598072

MING:

122076876

TSS:

122654711

PSOM:

113299904 113300303

OSA:

9266797

DOSA:

Os09t0411650-01(Os09g0411650)

OBR:

102703061

OGL:

127784497

BDI:

100823096

ATS:

109773851

TDC:	119301750 119309640

TAES:

123103906 123114815 123121680

TUA:

125509474

LPER:

127301336

LRD:	124654941 124655076

SBI:

8063304

ZMA:

100382373

SITA:

101771156

SVS:

117845324

PVIR:

120660633

PHAI:

112883419

PDA:

103714847

EGU:

105058571

MUS:	103992431 103992436

ZOF:	121969188 121974301

DCT:

110107875

PEQ:

110025947

AOF:

109826056

MSIN:

131230941

NCOL:

116248103

ATR:

18422052

SMO:	SELMODRAFT_119151 SELMODRAFT_449995

PPP:

112279922

CRE:	CHLRE_16g661350v5

VCN:	VOLCADRAFT_109735(VRMT1)

MNG:

MNEG_7683

CSL:	COCSUDRAFT_19420

CVR:	CHLNCDRAFT_8187

OLU:	OSTLU_43434

OTA:	OT_ostta14g00200

BPG:	Bathy04g04850

MIS:	MICPUN_63742

MPP:	MICPUCDRAFT_36635(JGI:MicpuC2_36635)

CME:	CYME_CMJ178C

GSL:	Gasu_15050

CCP:	CHC_T00002187001

NGD:	NGA_2061300

PIF:	PITG_00531

PSOJ:

PHYSODRAFT_561468

SPAR:

SPRG_03390

EHX:	EMIHUDRAFT_448733

» show all

Reference

1 [PMID:8527940]

Authors

Wang P, Royer M, Houtz RL.

Title

Affinity purification of ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase.

Journal

Protein Expr Purif 6:528-36 (1995)
DOI:10.1006/prep.1995.1070

Reference

2 [PMID:8980518]

Authors

Ying Z, Janney N, Houtz RL

Title

Organization and characterization of the ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase gene in tobacco.

Journal

Plant Mol Biol 32:663-71 (1996)
DOI:10.1007/BF00020207

Sequence

[ag:AAC49566]

Reference

3 [PMID:17338551]

Authors

Dirk LM, Flynn EM, Dietzel K, Couture JF, Trievel RC, Houtz RL

Title

Kinetic manifestation of processivity during multiple methylations catalyzed by SET domain protein methyltransferases.

Journal

Biochemistry 46:3905-15 (2007)
DOI:10.1021/bi6023644

Reference

4 [PMID:17635932]

Authors

Magnani R, Nayak NR, Mazarei M, Dirk LM, Houtz RL

Title

Polypeptide substrate specificity of PsLSMT. A set domain protein methyltransferase.

Journal

J Biol Chem 282:27857-64 (2007)
DOI:10.1074/jbc.M702069200

Reference

5 [PMID:22547063]

Authors

Mininno M, Brugiere S, Pautre V, Gilgen A, Ma S, Ferro M, Tardif M, Alban C, Ravanel S

Title

Characterization of chloroplastic fructose 1,6-bisphosphate aldolases as lysine-methylated proteins in plants.

Journal

J Biol Chem 287:21034-44 (2012)
DOI:10.1074/jbc.M112.359976

Sequence

[ag:AAA69903]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.127

IUBMB Enzyme Nomenclature:

2.1.1.127

ExPASy - ENZYME nomenclature database:

2.1.1.127

BRENDA, the Enzyme Database:

2.1.1.127

CAS:	139171-98-5

LinkDB

DBGET integrated database retrieval system