KEGG ENZYME: 2.1.1.150

ENZYME: 2.1.1.150

Entry

EC 2.1.1.150                Enzyme

Name

isoflavone 7-O-methyltransferase

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:hydroxyisoflavone 7-O-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + a 7-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 7-methoxyisoflavone [RN:R07375]

Reaction(KEGG)

R07375 > R06794 R07724

Substrate

S-adenosyl-L-methionine [CPD:C00019];
7-hydroxyisoflavone [CPD:C15615]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
7-methoxyisoflavone [CPD:C15616]

Comment

The enzyme from alfalfa can methylate daidzein, genistein and 6,7,4'-trihydroxyisoflavone but not flavones or flavanones.

History

EC 2.1.1.150 created 2003

Pathway

ec00943

Isoflavonoid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K13262

isoflavone-7-O-methyltransferase

Genes

GMX:	100776336 100779638 100791825 100800021 100800912 100811609 100812688 100813225 100818724 100818947 100819257 100819796 100819853 100819957

GSJ:	114366990 114366991 114369283 114370383 114370796 114395435 114395780 114395852 114395926 114402360 114402638 114402878 114422730

PVU:	PHAVU_007G114000g PHAVU_008G032600g PHAVU_008G032700g

VRA:	106758582 106759051 106759056 106771418 111241498

VAR:	108323040 108323056

VUN:	114182971 114183378 114186064 114186125 114186248

VUM:	124830651 124830652

CCAJ:

109795035 109802220 109802855 109802960 109818703

APRC:

113863219 113872610 113872612

MTR:	112416506 11419743 11430715 25484505 25484506 25493088 25493089

TPRA:

123889968 123893209 123910408 123914018 123914031 123916699

CAM:

101505606

PSAT:

127128641 127128642 127128643 127128644 127128645

VVO:	131608885 131608886 131608887 131611220

LJA:	Lj1g3v3023790.1(Lj1g3v3023790.1)

ADU:	107463257 107464082 107486238 107486358 107486360 107486373 107486439 107486555 107486622 107486623 107486645 107489657 127739569 127739570

AIP:	107612696 107635412 107635414 107635489 107635490 107635608 107635609 107638164 107638166 107638167 107638188 107644738

AHF:

112707909 112709830 112740687 112740691 112740702 112740703 112740704 112740705 112744553 112744558 112744580 112744581 112744582 112744659 112744806 112744812 112744813 112744814 112751677 112772937 112794481 112794482 112794487 112794489 112794490 112794491 112794492 112794493 112797857 112797874 112797876 112798095 112798101 112798102 112798104 112801706 114927210 114927646

LANG:

109329173 109333424 109350006 109353780

PCIN:

129284810 129284881 129284916 129286551 129286552 129286555 129286556 129286568 129287311 129298903 129317389 129318020 129318021 129318022 129318025

QSA:	O6P43_020298 O6P43_031889 O6P43_031890 O6P43_031892 O6P43_031894

» show all

Reference

Authors

Edwards, R. and Dixon, R.A.

Title

Isoflavone O-methyltransferase activities in elicitor-treated cell suspension cultures of Medicago sativa.

Journal

Phytochemistry 30:2597-2606 (1991)

Reference

2 [PMID:11006341]

Authors

He XZ, Dixon RA.

Title

Genetic manipulation of isoflavone 7-O-methyltransferase enhances biosynthesis of 4'-O-methylated isoflavonoid phytoalexins and disease resistance in alfalfa.

Journal

Plant Cell 12:1689-702 (2000)
DOI:10.1105/tpc.12.9.1689

Reference

3 [PMID:8951042]

Authors

He XZ, Dixon RA

Title

Affinity chromatography, substrate/product specificity, and amino acid sequence analysis of an isoflavone O-methyltransferase from alfalfa (Medicago sativa L.).

Journal

Arch Biochem Biophys 336:121-9 (1996)
DOI:10.1006/abbi.1996.0539

Sequence

[ag:AAC49928]

Reference

4 [PMID:9484461]

Authors

He XZ, Reddy JT, Dixon RA

Title

Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase.

Journal

Plant Mol Biol 36:43-54 (1998)
DOI:10.1023/A:1005938121453

Sequence

[ag:AAC49926 AAC49927 AAC49928]

Reference

5 [PMID:11752378]

Authors

Liu CJ, Dixon RA.

Title

Elicitor-induced association of isoflavone O-methyltransferase with endomembranes prevents the formation and 7-O-methylation of daidzein during isoflavonoid phytoalexin biosynthesis.

Journal

Plant Cell 13:2643-58 (2001)
DOI:10.1105/tpc.010382

Reference

6 [PMID:11224575]

Authors

Zubieta C, He XZ, Dixon RA, Noel JP.

Title

Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases.

Journal

Nat Struct Biol 8:271-9 (2001)
DOI:10.1038/85029

Sequence

[ag:AAC49928]