KEGG ENZYME: 2.3.1.175

ENZYME: 2.3.1.175

Entry

EC 2.3.1.175                Enzyme

Name

deacetylcephalosporin-C acetyltransferase;
acetyl-CoA:deacetylcephalosporin-C acetyltransferase;
DAC acetyltransferase;
cefG;
deacetylcephalosporin C acetyltransferase;
acetyl coenzyme A:DAC acetyltransferase;
acetyl-CoA:DAC acetyltransferase;
CPC acetylhydrolase;
acetyl-CoA:DAC O-acetyltransferase;
DAC-AT

Class

Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups

Sysname

acetyl-CoA:deacetylcephalosporin-C O-acetyltransferase

Reaction(IUBMB)

acetyl-CoA + deacetylcephalosporin C = CoA + cephalosporin C [RN:R03064]

Reaction(KEGG)

R03064

Substrate

acetyl-CoA [CPD:C00024];
deacetylcephalosporin C [CPD:C03112]

Product

CoA [CPD:C00010];
cephalosporin C [CPD:C00916]

Comment

This enzyme catalyses the final step in the biosynthesis of cephalosporin C.

History

EC 2.3.1.175 created 2005

Pathway

ec00311

Penicillin and cephalosporin biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K12747

deacetylcephalosporin-C acetyltransferase

Reference

1 [PMID:1368946]

Authors

Matsuyama K, Matsumoto H, Matsuda A, Sugiura H, Komatsu K, Ichikawa S.

Title

Purification of acetyl coenzyme A: deacetylacephalosporin C O-acetyltransferase from Acremonium chrysogenum.

Journal

Biosci Biotechnol Biochem 56:1410-2 (1992)
DOI:10.1271/bbb.56.1410

Reference

2 [PMID:1569032]

Authors

Gutierrez S, Velasco J, Fernandez FJ, Martin JF

Title

The cefG gene of Cephalosporium acremonium is linked to the cefEF gene and encodes a deacetylcephalosporin C acetyltransferase closely related to homoserine O-acetyltransferase.

Journal

J Bacteriol 174:3056-64 (1992)
DOI:10.1128/JB.174.9.3056-3064.1992

Sequence

[ag:AAA32673]

Reference

3 [PMID:1632779]

Authors

Matsuda A, Sugiura H, Matsuyama K, Matsumoto H, Ichikawa S, Komatsu K

Title

Cloning and disruption of the cefG gene encoding acetyl coenzyme A: deacetylcephalosporin C o-acetyltransferase from Acremonium chrysogenum.

Journal

Biochem Biophys Res Commun 186:40-6 (1992)
DOI:10.1016/S0006-291X(05)80772-7

Sequence

[ag:AAA32673]

Reference

4 [PMID:9421924]

Authors

Gutierrez S, Velasco J, Marcos AT, Fernandez FJ, Fierro F, Barredo JL, Diez B, Martin JF.

Title

Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum.

Journal

Appl Microbiol Biotechnol 48:606-14 (1997)
DOI:10.1007/s002530051103

Reference

5 [PMID:9895280]

Authors

Velasco J, Gutierrez S, Campoy S, Martin JF.

Title

Molecular characterization of the Acremonium chrysogenum cefG gene product: the native deacetylcephalosporin C acetyltransferase is not processed into subunits.

Journal

Biochem J 337 ( Pt 3):379-85 (1999)

Reference

6 [PMID:7847890]

Authors

Martin JF, Gutierrez S, Fernandez FJ, Velasco J, Fierro F, Marcos AT, Kosalkova K.

Title

Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins.

Journal

Antonie Van Leeuwenhoek 65:227-43 (1994)
DOI:10.1007/bf00871951

Other DBs

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