KEGG ENZYME: 2.3.1.186

ENZYME: 2.3.1.186

Entry

EC 2.3.1.186                Enzyme

Name

pseudotropine acyltransferase;
pseudotropine:acyl-CoA transferase;
tigloyl-CoA:pseudotropine acyltransferase;
acetyl-CoA:pseudotropine acyltransferase;
pseudotropine acetyltransferase;
pseudotropine tigloyltransferase;
PAT (ambiguous)

Class

Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups

Sysname

acyl-CoA:pseudotropine O-acyltransferase

Reaction(IUBMB)

an acyl-CoA + pseudotropine = CoA + an O-acylpseudotropine [RN:R08713]

Reaction(KEGG)

R08713 > R06736

Substrate

acyl-CoA [CPD:C00040];
pseudotropine [CPD:C02066]

Product

CoA [CPD:C00010];
O-acylpseudotropine [CPD:C17378]

Comment

This enzyme exhibits absolute specificity for the exo/3beta configuration found in pseudotropine as tropine (tropan-3alpha-ol; see EC 2.3.1.185, tropine acyltransferase) and nortropine are not substrates [1]. Acts on a wide range of aliphatic acyl-CoA derivatives, including acetyl-CoA, beta-methylcrotonyl-CoA and tigloyl-CoA [1].

History

EC 2.3.1.186 created 2008

Pathway

ec00960

Tropane, piperidine and pyridine alkaloid biosynthesis

Reference

Authors

Rabot, S., Peerless, A.C.J. and Robins, R.J.

Title

Tigloyl-CoA:pseudotropine acyltransferase - an enzyme of tropane alkaloid biosynthesis.

Journal

Phytochemistry 39:315-322 (1995)

Reference

2 [PMID:1959620]

Authors

Robins RJ, Bachmann P, Robinson T, Rhodes MJ, Yamada Y.

Title

The formation of 3 alpha- and 3 beta-acetoxytropanes by Datura stramonium transformed root cultures involves two acetyl-CoA-dependent acyltransferases.

Journal

FEBS Lett 292:293-7 (1991)
DOI:10.1016/0014-5793(91)80887-9

Reference

Authors

Robins, R.J., Bachmann,P., Peerless, A.C.J. and Rabot, S.

Title

Esterification reactions in the biosynthesis of tropane alkaloids in transformed root cultures.

Journal

Plant Cell, Tissue Organ Cult 38:241-247 (1994)

Reference

4 [PMID:10626376]

Authors

Boswell HD, Drager B, McLauchlan WR, Portsteffen A, Robins DJ, Robins RJ, Walton NJ.

Title

Specificities of the enzymes of N-alkyltropane biosynthesis in Brugmansia and Datura.

Journal

Phytochemistry 52:871-8 (1999)
DOI:10.1016/S0031-9422(99)00293-9

Other DBs

ExplorEnz - The Enzyme Database:

2.3.1.186

IUBMB Enzyme Nomenclature:

2.3.1.186

ExPASy - ENZYME nomenclature database:

2.3.1.186

BRENDA, the Enzyme Database:

2.3.1.186

CAS:	138440-78-5 162535-26-4

LinkDB

DBGET integrated database retrieval system