Entry
Name
alpha,alpha-trehalose synthase;
trehalose synthase;
trehalose synthetase;
UDP-glucose:glucose 1-glucosyltransferase;
TreT;
PhGT;
ADP-glucose:D-glucose 1-alpha-D-glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
NDP-alpha-D-glucose:D-glucose 1-alpha-D-glucosyltransferase
Reaction(IUBMB)
NDP-alpha-D-glucose + D-glucose = alpha,alpha-trehalose + NDP [RN:
R10525 ]
Reaction(KEGG)
Substrate
Product
Comment
Requires Mg2+ for maximal activity [1]. The enzyme-catalysed reaction is reversible [1]. In the reverse direction to that shown above, the enzyme is specific for alpha,alpha-trehalose as substrate, as it cannot use alpha- or beta-paranitrophenyl glucosides, maltose, sucrose, lactose or cellobiose [1]. While the enzymes from the thermophilic bacterium Rubrobacter xylanophilus and the hyperthermophilic archaeon Pyrococcus horikoshii can use ADP-, UDP- and GDP-alpha-D-glucose to the same extent [2,3], that from the hyperthermophilic archaeon Thermococcus litoralis has a marked preference for ADP-alpha-D-glucose [1] and that from the hyperthermophilic archaeon Thermoproteus tenax has a marked preference for UDP-alpha-D-glucose [4].
History
EC 2.4.1.245 created 2008, modified 2013
Pathway
ec00500 Starch and sucrose metabolism
Orthology
Genes
SAME : SAMCFNEI73_pB0408(treT)
DAO : Desac_0491 Desac_2074
DTI : Desti_0427 Desti_1352
CCX : COCOR_06731(treT) COCOR_07114(treT1)
MFU : LILAB_00130 LILAB_04900
MMAS : MYMAC_000794 MYMAC_001828
AVM : JQX13_08250 JQX13_21010
VIN : AKJ08_0183 AKJ08_2261
LLU : AKJ09_03277 AKJ09_03923
AHW : NCTC11636_01420(treT)
AIR : NCTC12972_01655(treT)
ASLA : NCTC11923_00301(treT)
AVC : NCTC10951_01814(treT)
RUB : GBA63_16795 GBA63_18845
BSOL : FSW04_00020 FSW04_25355
BALA : DSM104299_05800(treT)
SBAE : DSM104329_02677(treT_1) DSM104329_05713(treT_2)
KBB : ccbrp13_09000 ccbrp13_34900
JET : L3J17_03195 L3J17_15505
NKF : Nkreftii_002979 Nkreftii_003019
BIH : BIP78_0183 BIP78_0300
MEM : Memar_1192 Memar_1763 Memar_2023
MBG : BN140_0244 BN140_0628 BN140_1261
MEMA : MMAB1_0304(treT) MMAB1_0814(treT) MMAB1_1593(treT)
MCHK : MchiMG62_13560 MchiMG62_20220 MchiMG62_23340
MSUM : OH143_00180 OH143_03030 OH143_04385
HRU : Halru_0712 Halru_2965
» show all
Taxonomy
Reference
Authors
Qu Q, Lee SJ, Boos W.
Title
TreT, a novel trehalose glycosyltransferring synthase of the hyperthermophilic archaeon Thermococcus litoralis.
Journal
Sequence
Reference
Authors
Ryu SI, Park CS, Cha J, Woo EJ, Lee SB
Title
A novel trehalose-synthesizing glycosyltransferase from Pyrococcus horikoshii: molecular cloning and characterization.
Journal
Sequence
Reference
Authors
Nobre A, Alarico S, Fernandes C, Empadinhas N, da Costa MS
Title
A unique combination of genetic systems for the synthesis of trehalose in Rubrobacter xylanophilus: properties of a rare actinobacterial TreT.
Journal
Sequence
Reference
Authors
Kouril T, Zaparty M, Marrero J, Brinkmann H, Siebers B
Title
A novel trehalose synthesizing pathway in the hyperthermophilic Crenarchaeon Thermoproteus tenax: the unidirectional TreT pathway.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 2.4.1.245
LinkDB
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