KEGG   ENZYME: 2.5.1.65
Entry
EC 2.5.1.65                 Enzyme                                 
Name
O-phosphoserine sulfhydrylase;
O-phosphoserine(thiol)-lyase
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
O-phospho-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase
Reaction(IUBMB)
O-phospho-L-serine + hydrogen sulfide = L-cysteine + phosphate [RN:R07274]
Reaction(KEGG)
R07274;
(other) R00897
Substrate
O-phospho-L-serine [CPD:C01005];
hydrogen sulfide [CPD:C00283]
Product
L-cysteine [CPD:C00097];
phosphate [CPD:C00009]
Comment
A pyridoxal-phosphate protein. The enzyme from Aeropyrum pernix acts on both O-phospho-L-serine and O3-acetyl-L-serine, in contrast with EC 2.5.1.47, cysteine synthase, which acts only on O3-acetyl-L-serine.
History
EC 2.5.1.65 created 2004
Pathway
ec00270  Cysteine and methionine metabolism
ec00920  Sulfur metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01120  Microbial metabolism in diverse environments
Orthology
K10150  cysteine synthase / O-phosphoserine sulfhydrylase / cystathionine beta-synthase
Genes
APEAPE_1586(cysK)
ACJACAM_0997(cysK)
DFDDesfe_0630
DMUDesmu_0644
TAGTagg_1314
IAGIgag_1783
THGTCELL_0171
HBUHbut_1585
PFMPyrfu_1108
PDLPyrde_0768
PABIPABY_05110
PISPisl_1761
TPETpen_1605
THBN186_00700
TCBTCARB_1222
ASCASAC_0793
ACIASE86_05800
CLGCalag_0796
CCAINAS2_0810
 » show all
Reference
1  [PMID:12965218]
  Authors
Mino K, Ishikawa K.
  Title
A novel O-phospho-L-serine sulfhydrylation reaction catalyzed by O-acetylserine sulfhydrylase from Aeropyrum pernix K1.
  Journal
FEBS Lett 551:133-8 (2003)
DOI:10.1016/S0014-5793(03)00913-X
Reference
2  [PMID:12644499]
  Authors
Mino K, Ishikawa K
  Title
Characterization of a novel thermostable O-acetylserine sulfhydrylase from Aeropyrum pernix K1.
  Journal
J Bacteriol 185:2277-84 (2003)
DOI:10.1128/JB.185.7.2277-2284.2003
  Sequence
[ape:APE_1586]
Reference
3  [PMID:12554945]
  Authors
Mino K, Oda Y, Ataka M, Ishikawa K.
  Title
Crystallization and preliminary X-ray diffraction analysis of O-acetylserine sulfhydrylase from Aeropyrum pernix K1.
  Journal
Acta Crystallogr D Biol Crystallogr 59:338-40 (2003)
DOI:10.1107/S0907444902017900
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.65
IUBMB Enzyme Nomenclature: 2.5.1.65
ExPASy - ENZYME nomenclature database: 2.5.1.65
BRENDA, the Enzyme Database: 2.5.1.65
CAS: 1071505-11-7
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