KEGG   ENZYME: 2.7.7.46
Entry
EC 2.7.7.46                 Enzyme                                 
Name
gentamicin 2''-nucleotidyltransferase;
gentamicin 2''-adenylyltransferase;
aminoglycoside adenylyltransferase;
gentamycin 2''-nucleotidyltransferase
Class
Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases
Sysname
NTP:gentamicin 2''-nucleotidyltransferase
Reaction(IUBMB)
nucleoside triphosphate + gentamicin = diphosphate + 2''-nucleotidylgentamicin [RN:R02432]
Reaction(KEGG)
R02432 > R09047
Substrate
nucleoside triphosphate [CPD:C00201];
gentamicin [CPD:C00505]
Product
diphosphate [CPD:C00013];
2''-nucleotidylgentamicin [CPD:C03565]
Comment
ATP, dATP, CTP, ITP and GTP can act as donors; kanamycin, tobramycin and sisomicin can also act as acceptors. The nucleotidyl residue is transferred to the 2-hydroxy of the 3-amino-3-deoxy-D-glucose moiety in the antibiotic.
History
EC 2.7.7.46 created 1976
Orthology
K17881  aminoglycoside 2''-adenylyltransferase
Genes
ECLE: ECNIH2_24600 ECNIH2_25855
ECLN: ECNIH4_23065
ECLI: ECNIH5_22890
KPN: KPN_pKPN5p08204
KPZ: KPNIH27_26410 KPNIH27_27275
KOK: KONIH1_31180
KOC: AB185_01215
KQU: AVR78_27585
KQV: B8P98_29915
CFD: CFNIH1_06100 CFNIH1_25705
CIR: C2U53_00275(aadB)
KIN: AB182_00470
EBC: C2U52_00390(aadB) C2U52_01405(aadB)
PTHA: OI982_21545(aadB)
PHAG: PZ638_20805(aadB)
PMP: Pmu_03410(aadB)
PMEX: H4W19_04355(aadB)
PPUH: B479_27254
PALD: LU682_024155(aadB)
DCE: O6P33_04425(aadB)
ABY: ABAYE3622(aadB)
ABAL: ABLAC_p200010
AVZ: HWI77_18550(aadB)
WCN: PE074_09975(aadB)
NBL: GJV52_06000(aadB)
 » show all
Reference
1  [PMID:6281224]
  Authors
Angelatou F, Litsas SB, Kontomichalou P.
  Title
Purification and properties of two gentamicin-modifying enzymes, coded by a single plasmid pPK237 originating from Pseudomonas aeruginosa.
  Journal
J Antibiot (Tokyo) 35:235-44 (1982)
DOI:10.7164/antibiotics.35.235
Reference
2  [PMID:4946513]
  Authors
Naganawa H, Yagisawa M, Kondo S, Takeuchi T, Umezawa H.
  Title
The structure determination of an enzymatic inactivation product of 3',4'-dideoxykanamycin B.
  Journal
J Antibiot (Tokyo) 24:913-4 (1971)
DOI:10.7164/antibiotics.24.913
Reference
3  [PMID:4946512]
  Authors
Yagisawa M, Naganawa H, Kondo S, Hamada M, Takeuchi T.
  Title
Adenylyldideoxykanamycin B, a product of the inactivation of dideoxykanamycin B by Escherichia coli carrying R factor.
  Journal
J Antibiot (Tokyo) 24:911-2 (1971)
DOI:10.7164/antibiotics.24.911
Other DBs
ExplorEnz - The Enzyme Database: 2.7.7.46
IUBMB Enzyme Nomenclature: 2.7.7.46
ExPASy - ENZYME nomenclature database: 2.7.7.46
BRENDA, the Enzyme Database: 2.7.7.46
CAS: 62213-33-6
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