KEGG ENZYME: 3.5.3.21

ENZYME: 3.5.3.21

Entry

EC 3.5.3.21                 Enzyme

Name

methylenediurea deaminase;
methylenediurease

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amidines

Sysname

methylenediurea aminohydrolase

Reaction(IUBMB)

methylenediurea + 2 H2O = N-(hydroxymethyl)urea + 2 NH3 + CO2 (overall reaction) [RN:R05169];
(1a) methylenediurea + H2O = N-(carboxyaminomethyl)urea + NH3 [RN:R05170];
(1b) N-(carboxyaminomethyl)urea = N-(aminomethyl)urea + CO2 (spontaneous) [RN:R05171];
(1c) N-(aminomethyl)urea + H2O = N-(hydroxymethyl)urea + NH3 (spontaneous) [RN:R05172]

Reaction(KEGG)

R05169 R05170 R05171 R05172

Substrate

methylenediurea [CPD:C06381];
H2O [CPD:C00001];
N-(carboxyaminomethyl)urea [CPD:C06382];
N-(aminomethyl)urea [CPD:C06383]

Product

N-(hydroxymethyl)urea [CPD:C06384];
NH3 [CPD:C00014];
CO2 [CPD:C00011];
N-(carboxyaminomethyl)urea [CPD:C06382];
N-(aminomethyl)urea [CPD:C06383]

Comment

Methylenediurea is hydrolysed and decarboxylated to give an aminated methylurea, which then spontaneously hydrolyses to hydroxymethylurea. The enzyme from Ochrobactrum anthropi also hydrolyses dimethylenetriurea and trimethylenetetraurea as well as ureidoglycolate, which is hydrolysed to urea and glyoxylate, and allantoate, which is hydrolysed to ureidoglycolate, ammonia and carbon dioxide.

History

EC 3.5.3.21 created 1999

Reference

Authors

Jahns, T., Schepp, R., Kaltwasser, H.

Title

Purification and characterisation of an enzyme from a strain of Ochrobactrum anthropi that degrades condensation products of urea and formaldehyde (ureaform).

Journal

Can J Microbiol 43:1111-1117 (1997)

Other DBs

ExplorEnz - The Enzyme Database:

3.5.3.21

IUBMB Enzyme Nomenclature:

3.5.3.21

ExPASy - ENZYME nomenclature database:

3.5.3.21

BRENDA, the Enzyme Database:

3.5.3.21

CAS:	205830-62-2

LinkDB

DBGET integrated database retrieval system