Entry
Name
trans-o-hydroxybenzylidenepyruvate hydratase-aldolase;
2'-hydroxybenzalpyruvate aldolase;
NsaE;
tHBPA hydratase-aldolase
Class
Lyases;
Carbon-carbon lyases;
Aldehyde-lyases
BRITE hierarchy
Sysname
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate hydro-lyase
Reaction(IUBMB)
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O = salicylaldehyde + pyruvate [RN:
R05136 ]
Reaction(KEGG)
Substrate
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate [CPD:
C06203 ];
H2O [CPD:
C00001 ]
Product
Comment
This enzyme is involved in naphthalene degradation. The enzyme catalyses a retro-aldol reaction in vitro, and it accepts a broad range of aldehydes and 4-substituted 2-oxobut-3-enoates as substrates [4].
History
EC 4.1.2.45 created 2010, modified 2011
Pathway
ec01120 Microbial metabolism in diverse environments
Orthology
K14585 trans-o-hydroxybenzylidenepyruvate hydratase-aldolase
Genes
GBI : PG2T_00740 PG2T_05760 PG2T_12860
BUQ : AC233_10870 AC233_11110
BFN : OI25_3035 OI25_3053(phdJ)
RBU : PG1C_04265 PG1C_08125
PGV : SL003B_4082 SL003B_4105
NPP : PP1Y_AT15847 PP1Y_AT31250
NPN : JI59_00960 JI59_04200 JI59_21720 JI59_21740 JI59_21760 JI59_21850 JI59_21910 JI59_24690
CNA : AB433_00715 AB433_10235 AB433_10410 AB433_18070
» show all
Taxonomy
Reference
Authors
Kuhm AE, Knackmuss HJ, Stolz A
Title
Purification and properties of 2'-hydroxybenzalpyruvate aldolase from a bacterium that degrades naphthalenesulfonates.
Journal
J Biol Chem 268:9484-9 (1993)
Reference
Authors
Keck A, Conradt D, Mahler A, Stolz A, Mattes R, Klein J
Title
Identification and functional analysis of the genes for naphthalenesulfonate catabolism by Sphingomonas xenophaga BN6.
Journal
Sequence
Reference
Authors
Eaton RW
Title
Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the NAH7 plasmid.
Journal
Sequence
Reference
Authors
Eaton RW
Title
trans-o-Hydroxybenzylidenepyruvate hydratase-aldolase as a biocatalyst.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 4.1.2.45
ExPASy - ENZYME nomenclature database: 4.1.2.45
LinkDB
All DBs