KEGG   ENZYME: 4.1.99.2
Entry
EC 4.1.99.2                 Enzyme                                 
Name
tyrosine phenol-lyase;
beta-tyrosinase;
L-tyrosine phenol-lyase (deaminating)
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
Sysname
L-tyrosine phenol-lyase (deaminating; pyruvate-forming)
Reaction(IUBMB)
L-tyrosine + H2O = phenol + pyruvate + NH3 (overall reaction) [RN:R00728];
(1a) L-tyrosine = phenol + 2-aminoprop-2-enoate [RN:R12759];
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous) [RN:R11100];
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous) [RN:R11099]
Reaction(KEGG)
R00728 R11099 R11100 R12759
Substrate
L-tyrosine [CPD:C00082];
H2O [CPD:C00001];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Product
phenol [CPD:C00146];
pyruvate [CPD:C00022];
NH3 [CPD:C00014];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Comment
A pyridoxal-phosphate protein. The enzyme cleaves a carbon-carbon bond, releasing phenol and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. The enzyme also slowly catalyses similar reactions with D-tyrosine, S-methyl-L-cysteine, L-cysteine, L-serine and D-serine.
History
EC 4.1.99.2 created 1972
Pathway
ec00350  Tyrosine metabolism
Orthology
K01668  tyrosine phenol-lyase
Genes
KQV: B8P98_13910
KAR: LGL98_12615
CKO: CKO_03467
CFD: CFNIH1_09255
CBRA: A6J81_13920
CWE: CO701_18800
CYO: CD187_21020
CPOT: FOB25_08080
CFQ: C2U38_02820
CIF: AL515_04345
CIE: AN232_00695
CPAR: CUC49_22445
CITZ: E4Z61_13950
CARS: E1B03_04065
CIX: M4I31_20605
CIB: HF677_020385
CENS: P2W74_20015
ASUB: NLZ15_03930
KIN: AB182_14870
PDZ: HHA33_17405
METY: MRY16398_33370(tpl)
EBC: C2U52_25405
YMA: DA391_12585
PAM: PANA_3984(tpl)
PLF: PANA5342_p10091(tpl)
PAJ: PAJ_p0025(tpl)
PAQ: PAGR_p073
PMAG: JI723_05445
MMK: MU9_2951
PRAG: EKN56_08045 EKN56_12310
LRI: NCTC12151_02412(tpl)
HPR: PARA_06000
PMU: PM0811
PMV: PMCN06_0880(tpl)
PUL: NT08PM_0453
PMP: Pmu_08910(tpl)
PMUL: DR93_1650
PSKY: A6B44_09725
AAP: NT05HA_0124
AAZ: ADJ80_05155
AAT: D11S_1940
AAO: ANH9381_0123
AAN: D7S_01988
AAH: CF65_02970
AACN: AANUM_2335
AACT: ACT75_02960
RPNE: NCTC8284_00313(tpl)
RHEY: FEE42_07995
VMI: AL543_07035
EAZ: JHT90_07445
MVS: MVIS_2967(tnaA)
MYA: MORIYA_1533(tnaA)
MCEL: LPW13_08815
KIM: G3T16_05495
THIM: KFB96_22295
ENW: MJO57_30775
DNO: DNO_0442
CHJ: NCTC10426_00445(tpl)
AFF: H3L97_00400
BGE: BC1002_6700
PTX: ABW99_12265
CDN: BN940_06946
COF: FOZ74_12245
SIMP: C6571_06710
RGE: RGE_20590(tnaA)
RBN: RBXJA2T_17611(tnaA)
OFO: BRW83_0351(tpl)
OVB: NB640_06150
OAL: NB647_01400
EBA: ebA605(tnaA)
APET: ToN1_35920
AZI: AzCIB_0931
AZD: CDA09_05540
TMZ: Tmz1t_1789
TCL: Tchl_2494
TAK: Tharo_1668
RPB: RPB_1966
RPC: RPC_2787
RPD: RPD_3423
RPE: RPE_2915
RPT: Rpal_4083
RPX: Rpdx1_1790
RVA: Rvan_2623
RLAC: QMO75_10805
BVR: BVIR_1448
BLAG: BLTE_20100(tpl)
MSC: BN69_2860
MBRY: B1812_17480
MROS: EHO51_02135
MTW: CQW49_09160
MECQ: MSC49_14560(tnaA)
PSF: PSE_0962(tnaA)
RSQ: Rsph17025_3640
CAZT: LV780_17545
RCP: RCAP_rcc00516(tpl) RCAP_rcc02309(tnaA)
RSU: NHU_02389(tnaA)
RHM: B5V46_07690
HNE: HNE_0810
RAP: RHOA_5189(tpl)
RPM: RSPPHO_03014
MAGX: XM1_0733(tpl)
MAGN: WV31_12030
CBLA: CBLAS_0788
ATP: ATR_1949(tnaA)
ATHR: ATH_1913(tnaA)
APOC: APORC_2028(tnaA)
SAT: SYN_01455
NANN: O0S08_25630
BWD: CT694_33960
BSHI: LGQ02_13810
SPAE: E2C16_02905
SPOO: J3U78_00095
SURL: BI350_06705
UTH: DKZ56_00685
CTC: CTC_00818
CTET: BN906_00858
CCOH: SAMEA4530647_0763(tpl_2)
CCAA: KQH81_12795
PROQ: P6M73_13460
CCL: Clocl_1892
HSC: HVS_04190(tpl)
RHER: EHE19_004455
RPAY: P0092_05030
STH: STH2842
DSY: DSY3653
DHD: Dhaf_1723
DDL: Desdi_0330
DOR: Desor_4416
DFG: B0537_00095 B0537_11110
ACAE: HYG86_05650
PROM: QO263_16355
CSH: Closa_0252
LBW: C3V36_04545
TEP: TepRe1_1876
TAE: TepiRe1_2021(tpl)
NTH: Nther_1802
NCD: ACONDI_01191(tpl_1) ACONDI_01327(tpl_2)
HPK: Hprae_1906
MHAP: JFY71_03580 JFY71_06480
TIS: P3962_14550
TIZ: RBU61_00645
PUF: UFO1_1418
PFT: JBW_03623
LPIL: LIP_0995
TBK: HF295_07920
ERZ: ER308_16190
CMD: B841_11865(tnaA)
CHM: B842_02140(tnaA)
CSPH: CSPHI_06785
CCYS: SAMEA4530656_1651(tnaA)
CFY: I6L56_04290
CGF: CGUA_12210(tpl)
CBRE: QP027_11135
CBQ: AL705_05405
SALU: DC74_716
SALL: SAZ_04135(tnaA)
SKA: CP970_07480
ICA: Intca_0142
ARS: ADJ73_00465
AUS: IPK37_00915 IPK37_04765
BRT: J4N02_01260 J4N02_04045
NCA: Noca_1624
AOI: AORI_5366
AJA: AJAP_22805(tpl)
AMYC: CU254_28905
AMYY: YIM_16040(tpl)
AORI: SD37_23450
AROO: NQK81_11530
RUB: GBA63_15970
CMP: Cha6605_2878
KOV: K9N68_37855
LSK: J5X98_22205
NPU: Npun_R5724
NOP: Nos7524_2080
NON: NOS3756_28400
NOE: CLI64_22335
TOQ: HCG51_14585
ALAX: NIES50_24750
RRS: RoseRS_0320
RCA: Rcas_0866
CAU: Caur_0573
CHL: Chy400_0619
CAG: Cagg_3438
PSUB: Pelsub_P0466
PBF: CFX0092_A2268(tnaA)
THUG: KNN16_10440
DFC: DFI_10615
OPR: Ocepr_1553 Ocepr_1720
MHD: Marky_1642
NPY: NPRO_01750 NPRO_16190
OTE: Oter_0376
ELUT: CKA38_03105
OBT: OPIT5_12280
AMU: Amuc_1995
ABIW: Abiwalacus_23920
AMAK: J5W79_10920
SMAM: Mal15_56580(tnaA2)
SACI: Sinac_1688
TDE: TDE_1118(tpl)
TPED: TPE_1499(tpl)
TPK: JO40_08670
TPHG: FUT81_00030
ABA: Acid345_3931
ORP: MOP44_23725
SUS: Acid_6369
PFER: IRI77_11825
ABAC: LuPra_04134(tpl)
THYD: TTHT_1544(tnaA)
SCOR: J3U87_27205
GEP: Q9293_13855
EMI: Emin_0932
FNU: FN1988
FNC: HMPREF0946_02086
FNT: HMPREF0405_01819
FUS: HMPREF0409_01828
FNE: FSDG_02356
FHW: RN87_01430 RN87_05525
FPD: CTM68_07230
FVA: FV113G1_10500 FV113G1_23210
FUL: C4N20_00420 C4N20_11335
FNF: BSQ88_03445
FPEI: C4N17_00230
FCI: I6I83_00260
FPOL: ERS445057_00135(tpl_1) ERS445057_01797(tpl_3)
CSOM: MKD34_06135
TAI: Taci_0642
ARAM: KAR29_11770
PCRE: NCTC12858_00848(tpl_1)
PCAG: NCTC12856_00106(tpl_1)
PEND: LA319_03700
PSOE: CE91St14_06700(tpl)
PBT: ING2E5B_1329
PMUC: ING2E5A_1495(tpl)
PET: PEIBARAKI_5473 PEIBARAKI_6464
PSAC: PSM36_1292 PSM36_2654(tpl)
PROJ: KDN43_00735 KDN43_09795
PPN: Palpr_1828
ALQ: C7Y71_008285
ALD: GFH31_00760
AIT: AI2BBH_02780(tpl)
RBC: BN938_2021
DORI: FH5T_08715
MCOS: GM418_17880
ANF: AQPE_1860
ASX: CDL62_16620
ALKQ: M9189_07190
COY: HF329_00540 HF329_11305
NYN: U0035_07050
HHY: Halhy_0884
SMIZ: 4412673_01210(tpl_2)
SPHZ: E3D81_15330
LUT: Lupro_11550
AQD: D1816_15070
WVI: Weevi_1094
ELT: FGE20_04745
CHZ: CHSO_0681(tnaA)
CGN: OK18_07025
CHH: A0O34_09960
CIO: CEQ15_17460
CPIP: CJF12_15000
CARH: EGY05_20955
CSHA: EG350_19400
CNK: EG343_13340
CGLE: NCTC11432_03144(tpl_1)
CTAK: 4412677_01735(tpl_2)
CSAL: NBC122_01726(tpl_2)
CSUP: MTP09_03975
CCAS: EIB73_09735
KDA: EIB71_04020
KFA: Q73A0000_00315
EVA: EIB75_11400
CNR: EB819_10635
EBV: F0358_05840
RMR: Rmar_1948
RMG: Rhom172_0915
RBAR: AWN76_012460
SRU: SRU_1708
SRM: SRM_01915(tnaA)
IAL: IALB_2618(tnaA)
MRO: MROS_0698 MROS_1546
DTN: DTL3_0081(tpl)
CABY: Cabys_2412
NMV: NITMOv2_0067(tnaA)
NJA: NSJP_0536(tnaA)
NIF: W02_04720(tnaA)
NALL: PP769_10995
NNEO: PQG83_01705
BANA: BARAN1_0718(tpl)
BIH: BIP78_0966
THG: TCELL_0288
PSYT: DSAG12_02524(tpl)
 » show all
Reference
1  [PMID:4908868]
  Authors
Kumagai H, Yamada H, Matsui H, Ohkishi H, Ogata K.
  Title
Tyrosine phenol lyase. I. Purification, crystallization, and properties.
  Journal
J Biol Chem 245:1767-72 (1970)
Reference
2  [PMID:4908869]
  Authors
Kumagai H, Yamada H, Matsui H, Ohkishi H, Ogata K.
  Title
Tyrosine phenol lyase. II. Cofactor requrements.
  Journal
J Biol Chem 245:1773-7 (1970)
Other DBs
ExplorEnz - The Enzyme Database: 4.1.99.2
IUBMB Enzyme Nomenclature: 4.1.99.2
ExPASy - ENZYME nomenclature database: 4.1.99.2
BRENDA, the Enzyme Database: 4.1.99.2
CAS: 9059-31-8
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