KEGG ENZYME: 4.3.1.9

ENZYME: 4.3.1.9

Entry

EC 4.3.1.9                  Enzyme

Name

glucosaminate ammonia-lyase;
glucosaminic dehydrase;
D-glucosaminate dehydratase;
D-glucosaminic acid dehydrase;
aminodeoxygluconate dehydratase;
2-amino-2-deoxy-D-gluconate hydro-lyase (deaminating);
aminodeoxygluconate ammonia-lyase;
2-amino-2-deoxy-D-gluconate ammonia-lyase;
D-glucosaminate ammonia-lyase;
D-glucosaminate ammonia-lyase (isomerizing;
2-dehydro-3-deoxy-D-gluconate-forming)

Class

Lyases;
Carbon-nitrogen lyases;
Ammonia-lyases

Sysname

2-amino-2-deoxy-D-gluconate ammonia-lyase (isomerizing; 2-dehydro-3-deoxy-D-gluconate-forming)

Reaction(IUBMB)

2-amino-2-deoxy-D-gluconate = 2-dehydro-3-deoxy-D-gluconate + NH3 (overall reaction) [RN:R01544];
(1a) 2-amino-2-deoxy-D-gluconate = (2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate + H2O [RN:R10773];
(1b) (2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate = (4S,5R)-4,5,6-trihydroxy-2-iminohexanoate (spontaneous) [RN:R10774];
(1c) (4S,5R)-4,5,6-trihydroxy-2-iminohexanoate + H2O = 2-dehydro-3-deoxy-D-gluconate + NH3 (spontaneous) [RN:R10775]

Reaction(KEGG)

R01544 R10773 R10774 R10775;
(other) R06132

Substrate

2-amino-2-deoxy-D-gluconate [CPD:C03752];
(2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate [CPD:C20844];
(4S,5R)-4,5,6-trihydroxy-2-iminohexanoate [CPD:C20845];
H2O [CPD:C00001]

Product

2-dehydro-3-deoxy-D-gluconate [CPD:C00204];
NH3 [CPD:C00014];
(2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate [CPD:C20844];
H2O [CPD:C00001];
(4S,5R)-4,5,6-trihydroxy-2-iminohexanoate [CPD:C20845]

Comment

Contains pyridoxal phosphate. The enzyme releases an unstable enamine product that tautomerizes to an imine form, which undergoes spontaneous hydrolytic deamination to form the final product.

History

EC 4.3.1.9 created 1972, (EC 4.3.1.21 created 1965 as EC 4.2.1.26, transferred 2002 to EC 4.3.1.21, incorporated 2004) modified 2004

Pathway

ec00030

Pentose phosphate pathway

ec01100

Metabolic pathways

Orthology

K22345

glucosaminate ammonia-lyase

Genes

PAE:	PA0849(trxB2)

PAEV:

N297_876(trxB)

PAEI:

N296_876(trxB)

PAU:	PA14_53290(trxB2)

PAP:	PSPA7_4671(trxB1)

PAG:	PLES_44691(trxB2)

PAF:	PAM18_4191(trxB2)

PNC:	NCGM2_1623(trxB2)

PAEB:

NCGM1900_1877(trxB2)

PDK:	PADK2_21460

PSG:	G655_21110

PRP:	M062_04130

PAEP:

PA1S_22200

PAER:

PA1R_gp4371

PAEM:

U769_21570

PAEL:

T223_22835

PAES:

SCV20265_4679

PAEU:

BN889_00876(trxB2)

PAEG:

AI22_11740

PAEC:

M802_872(trxB)

PAEO:

M801_876(trxB)

PMY:

Pmen_0978

PMK:

MDS_1066

PRE:	PCA10_47670(trxB)

PPSE:

BN5_3403

PFUW:

KF707C_47340

PALC:

A0T30_07600

PCQ:	PcP3B5_51170

PMUI:

G4G71_08585(trxB)

PNT:	G5B91_26485(trxB)

PPAA:

B7D75_21050(trxB)

PPU:	PP_0786(trxB)

PPF:

Pput_0809

PPG:	PputGB1_0820

PPW:	PputW619_4408

PPT:

PPS_0839

PPB:	PPUBIRD1_0830(trxB)

PPI:

YSA_06637

PPX:	T1E_2100(trxB)

PPUH:

B479_04405

PPUT:

L483_03945

PPUN:

PP4_44970(trxB)

PPUD:

DW66_0801

PFV:	Psefu_3370

PMON:

X969_02440

PMOT:

X970_02420

PPJ:	RK21_04166

POR:	APT59_03560

PMOL:

CLJ08_17245(trxB)

PALD:

LU682_004240(trxB)

PIX:	RIN61_15885(trxB)

PMOS:

O165_028095

PST:	PSPTO_1178(trxB)

PSB:

Psyr_1016

PSYR:

N018_20605

PSP:	PSPPH_1067(trxB)

PSAV:

PSA3335_08840

PAMG:

BKM19_007795(trxB)

PCI:	PCH70_09420

PAVL:

BKM03_06735(trxB)

PVD:	CFBP1590__4340

PCAB:

JGS08_05510(trxB)

PCOF:

POR16_19185(trxB)

PTRE:

I9H09_04835(trxB)

PSYI:

MME58_05020(trxB)

PAST:

N015_05715(trxB)

PLIJ:

KQP88_04535(trxB)

PFL:	PFL_0945(trxB_1)

PPRC:

PFLCHA0_c09600(trxB1)

PPRO:

PPC_0979

PFE:	PSF113_2176(trxB1) PSF113_4883(trxB2)

PFN:	HZ99_22980

PFO:	Pfl01_0887

PFS:

PFLU_5162

PFC:	PflA506_4454(trxB)

PPZ:	H045_16490

PFB:

VO64_2002

PMAN:

OU5_2518(trxB)

PTV:	AA957_19195

PCG:	AXG94_05355 AXG94_19945

PVR:	PverR02_26300

PAZO:

AYR47_01680

POI:	BOP93_21720

PRX:	HRH33_22510(trxB)

PMED:

E3Z27_04380(trxB)

PKR:	AYO71_07225

PMUD:

NCTC8068_00949(trxB)

PTOL:

I7845_24265(trxB)

PFW:	PF1751_v1c46110(trxB)

PFF:	PFLUOLIPICF712960

PFX:	A7318_21530

PEN:	PSEEN0924(trxB)

PLUL:

FOB45_09070(trxB)

PBA:	PSEBR_a3564 PSEBR_a4717

PBC:	CD58_04760

PPUU:

PputUW4_00805(trxB1)

PDR:	H681_19980

PSV:	PVLB_21190

PSK:	U771_26675

PKC:	PKB_4627(trxb3)

PCH:	EY04_04125

PCZ:	PCL1606_51480

PCP:	JM49_25560

PCHP:

C4K32_1027

PFZ:	AV641_18765

PLQ:	AA042_14850

PALK:

PSAKL28_45190

PRH:	LT40_20370

PSW:	LK03_09545

PPV:	NJ69_00675

PSES:

PSCI_0771

PSEM:

TO66_04840

PSEC:

CCOS191_4496

PPSY:

AOC04_15985

PSOS:

POS17_0950

PFK:	PFAS1_06720

PANR:

A7J50_4906

PPSL:

BJP27_17955

PSET:

THL1_4770

PSIL:

PMA3_24915

PYM:	AK972_4997

PADE:

C3B55_00980

PKE:	DLD99_04830(trxB)

PALL:

UYA_04805

PUM:	HGP31_04615(trxB)

POJ:	PtoMrB4_46880(trxB2)

PGG:	FX982_00732

PPSH:

G5J76_22520(trxB)

PGY:	AWU82_19430(trxB)

PLAL:

FXN65_22930(trxB)

PSEJ:

HNQ25_19360(trxB)

PVK:	EPZ47_04655(trxB)

PEZ:	HWQ56_04785(trxB)

PBZ:	GN234_23410(trxB)

PKH:	JLK41_05140(trxB)

PTY:	JWV26_15885(trxB)

PMAO:

PMYSY11_0858(trxB)

PATA:

JWU58_23070(trxB)

PDW:	BV82_0171(trxB)

PZE:	HU754_005895(trxB)

PMOE:

HV782_005500(trxB)

PCAM:

HNE05_11920(trxB)

PSEP:

C4K39_0964

PPII:

QL104_04775(trxB)

PVW:	HU752_027775(trxB)

PTRT:

HU722_0024570(trxB)

PXA:	KSS93_25260(trxB)

PSAM:

HU731_010990(trxB)

PTZ:	HU718_005555(trxB)

PQI:	KH389_04205(trxB)

PSHH:

HU773_005415(trxB)

PXN:	HU772_020750(trxB)

PHV:	HU739_013055(trxB)

PPRG:

HU725_019070(trxB)

PANH:

HU763_004140(trxB)

PASG:

KSS96_23885(trxB)

PFAK:

KSS94_22760(trxB)

PMUY:

KSS95_23555(trxB)

PMAM:

KSS90_21625(trxB)

PAZE:

KSS91_04870(trxB)

PALV:

KSS97_05625(trxB)

PORY:

EJA05_23790(trxB)

PWZ:	J7655_04615(trxB)

PTK:	EXN22_05660(trxB)

PTW:	TUM18999_50800(trxB2)

PZA:	HU749_024815(trxB)

PIE:	HU724_005340(trxB)

PWY:	HU734_021970(trxB)

PTAE:

NCTC10697_03611(trxB)

PGF:	J0G10_05685(trxB)

PCAV:

D3880_05200(trxB)

PJU:	L1P09_03745(trxB)

PHF:	NLY38_04950(trxB)

PIZ:	LAB08_R08100(trxB)

PPEG:

KUA23_14485(trxB) KUA23_25185(trxB)

PSII:

NF676_05020(trxB)

PSIH:

LOY51_22630(trxB)

PCAS:

LOY40_04560(trxB)

PFIT:

KJY40_05585(trxB)

PASI:

LG197_09400(trxB)

PKM:	PZ739_03970(trxB)

PNB:	NK667_22710(trxB)

PSOA:

PSm6_10660(trxB2)

PTAI:

ICN73_02520(trxB)

PKK:	QQ992_04320(trxB)

PKJ:	Q1W70_05800(trxB)

PHYG:

JTY93_04535(trxB)

PHOM:

KJF94_01255(trxB)

PCHE:

QYM18_12725(trxB)

PCUC:

PSH97_04330(trxB)

PTRL:

OU419_22880(trxB)

PPAE:

LDL65_06335(trxB)

PPAO:

K3169_23920(trxB)

PRHZ:

CRX69_08585(trxB)

PSJY:

AA098_23525

PSKU:

KUIN1_10540

PBB:	AKN87_04860

HPEG:

EAO82_18575(trxB)

HAES:

LO767_02085(trxB)

PDEN:

F1C79_18335(trxB)

HARR:

HV822_05170(trxB)

PNN:	KEM63_02915(trxB)

PPHA:

BVH74_07995

DCE:	O6P33_11215(trxB)

HCS:	FF32_03085

HAK:	KO116_00917

HAM:

HALO0949

HHH:	CLM76_03120(trxB)

HAG:	BB497_02120

HMD:	CTT34_15625(trxB)

HAXI:

HAALTHF_17720n

HPRO:

LMS44_18630(trxB)

HQD:	K1Y77_02345(trxB)

HBP:	HPTD01_1757

HALF:

QEN58_16105(trxB)

HASO:

B2G49_21095

HASH:

HXW73_14950(trxB)

HPIS:

P1P91_02680(trxB)

HALK:

CUU95_09030(trxB)

HVN:	EI420_17130(trxB)

HOL:	HORIV_57640(trxB2)

HSR:	HSBAA_55260(trxB2)

HPIZ:

GYM47_14845(trxB)

HNP:	SR894_17230(trxB)

HTT:	HZS52_09595(trxB)

DNO:	DNO_1099(trxB)

CHJ:	NCTC10426_00934(trxB)

NWE:	SAMEA3174300_0977(trxB)

NSI:	A6J88_08345(trxB)

NMJ:	NM96_03025(trxB)

NEI:	BG910_11830(trxB)

NSF:	FAH66_02675(trxB)

NZL:	D0T92_06925(trxB)

KKI:	KKKWG1_1505(trxB)

SMUR:

BWP33_08650

PLG:	NCTC10937_04342(trxB)

AXY:	AXYL_00105(trxB1)

SUTT:

SUTMEG_20070(trxB)

SUTK:

FG381_06245(trxB)

SWS:	I6J16_12290(trxB)

SECH:

B18_15890

GLO:

Glov_3350

PARH:

I5S86_24710(trxB)

HSU:	HLASF_1657(trxB2)

HSF:	HLASA_1644(trxB2)

HJE:	HacjB3_09255

HHI:	HAH_1736(trxB2)

HHN:	HISP_08860

HAB:	SG26_16590

HTA:	BVU17_08370

HWA:	HQ_1652A(trxB2)

HWC:	Hqrw_1767(trxB2)

HME:	HFX_1131(trxB)

HGI:	ABY42_05470

HALE:

G3A49_08835

HAER:

DU502_08980

HRA:	EI982_04615

HLM:	DV707_08530

» show all

Reference

Authors

Imanaga, Y.

Title

Metabolism of D-glucosamine. III. Enzymic degradation of D-glucosaminic acid.

Journal

J Biochem (Tokyo) 45:647-650 (1958)

Reference

Authors

Merrick, J.M. and Roseman, S.

Title

D-Glucosaminic acid dehydrase.

Journal

Methods Enzymol 9:657-660 (1966)

Reference

3 [PMID:7068563]

Authors

Iwamoto R, Imanaga Y, Soda K.

Title

D-glucosaminate dehydratase from Agrobacterium radiobacter. Physicochemical and enzymological properties.

Journal

J Biochem (Tokyo) 91:283-9 (1982)
DOI:10.1093/oxfordjournals.jbchem.a133686

Reference

4 [PMID:7766176]

Authors

Iwamoto R, Taniki H, Koishi J, Nakura S.

Title

D-glucosaminate aldolase activity of D-glucosaminate dehydratase from Pseudomonas fluorescens and its requirement for Mn2+ ion.

Journal

Biosci Biotechnol Biochem 59:408-11 (1995)
DOI:10.1271/bbb.59.408

Other DBs

ExplorEnz - The Enzyme Database:

4.3.1.9

IUBMB Enzyme Nomenclature:

4.3.1.9

ExPASy - ENZYME nomenclature database:

4.3.1.9

BRENDA, the Enzyme Database:

4.3.1.9

CAS:	37290-91-8

LinkDB

DBGET integrated database retrieval system