KEGG ENZYME: 5.5.1.13

ENZYME: 5.5.1.13

Entry

EC 5.5.1.13                 Enzyme

Name

ent-copalyl diphosphate synthase;
ent-kaurene synthase A;
ent-kaurene synthetase A;
ent-CDP synthase;
ent-copalyl-diphosphate lyase (decyclizing)

Class

Isomerases;
Intramolecular lyases;
Intramolecular lyases (only sub-subclass identified to date)

Sysname

ent-copalyl-diphosphate lyase (ring-opening)

Reaction(IUBMB)

geranylgeranyl diphosphate = ent-copalyl diphosphate [RN:R02068]

Reaction(KEGG)

R02068

Substrate

geranylgeranyl diphosphate [CPD:C00353]

Product

ent-copalyl diphosphate [CPD:C06089]

Comment

Part of a bifunctional enzyme involved in the biosynthesis of kaurene. See also EC 4.2.3.19 (ent-kaurene synthase)

History

EC 5.5.1.13 created 2002

Pathway

ec00904

Diterpenoid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K04120

ent-copalyl diphosphate synthase

K20657

ent-copalyl diphosphate/ent-kaurene synthase

Genes

ATH:	AT4G02780(GA1)

ALY:

9310964

CRB:

17883900

CSAT:

104707805 104714573 104737446

EUS:	EUTSA_v10029352mg

BRP:	103836713 103860100

BNA:	106392263 106426697 106443785 125583685

BOE:

106313827

RSZ:	108826010 130501278

THJ:

104801236

CPAP:

110814715

CIT:	102620281 102623063

CIC:	CICLE_v10018875mg CICLE_v10023706mg

PVY:	116130601 116130606 116130619

MINC:

123211143 123223998

TCC:

18613340

GRA:	105790720 105803113

GHI:	107890714 107908163 107912438 107923002

GAB:	108468330 108472513

HSYR:

120143650 120167627

DZI:	111283716 111306089 111313423

EGR:	104456992 104456994 120286860

GMX:	100784193(TPS22) 100807519(TPS1) 100808585(TPS2)

GSJ:	114398379 114406861

PVU:	PHAVU_001G151900g PHAVU_001G152100g

VRA:	106757084 106759618

VAR:	108347906 108347925

VUN:	114172203 114190781

VUM:	124840706 124845683

CCAJ:

109797402 109797984

APRC:

113862114 113862726

MTR:	11422426 25497503

TPRA:

123888694 123892879

CAM:	101495301 101496179

PSAT:

127128589

VVO:	131608821 131611203

LJA:	Lj0g3v0251619.1(Lj0g3v0251619.1) Lj1g3v0682850.3(Lj1g3v0682850.3) Lj1g3v0682850.4(Lj1g3v0682850.4) Lj1g3v4434270.1(Lj1g3v4434270.1) Lj1g3v4434270.3(Lj1g3v4434270.3)

ADU:	107472867 107492993 107493023

AIP:	107645177 107645180 107645483

AHF:	112696212 112696215 112720124 112756347 112756444 112791136 112791164

LANG:

109333831 109334928

PCIN:

129287858 129296245 129306848 129314894 129314914 129315338 129315421 129316183 129316332 129316382 129316404 129316521 129316523

QSA:	O6P43_007022 O6P43_007023 O6P43_007026 O6P43_010625 O6P43_026688 O6P43_026689 O6P43_026693 O6P43_026700

FVE:	101304451 101311730 101312317 105350378

RCN:	112168942 112169264

PPER:

18767747

PMUM:

103334237

PAVI:

110749801

PDUL:

117637679

MDM:	103448353 103448995(CPS)

MSYL:

126590981 126591449 126615623

PXB:	103957280 103960128

ZJU:	107426403 107429475

MNT:

21407792

CSAV:

115703738

CSV:

101214392

CMO:

103491661

BHJ:

120077469

MCHA:

111025355

CMAX:

111466926 111491077

CMOS:

111437474 111464066

CPEP:

111776354 111795944

RCU:

8272032

JCU:

105649988

HBR:	110643292 110644361 110664957 110669087 110673038

MESC:

110614866 110622660

POP:	7464838 7480038

PEU:	105119675 105126847 105126849 105131430

PALZ:

118049193 118061693

JRE:	108981932 109018179 109018342 109018449 109020261

CILL:

122315038 122318277

CAVE:

132170193 132170321 132171730

QSU:

112000653

QLO:

115969891

TWL:	119981709 119981710 119982058 119989499 119990080 119994944 119995644

VVI:	100259570 100264747

VRI:	117903922 117904719 117917632 117918245 117934442

SLY:	544303(CPS)

SPEN:

107021672

SOT:	102585386 102596242

SSTN:

125869604 125873119

SDUL:

129900365

CANN:

107874554

LBB:	132608089 132610116

NTA:	107809496 107819352 107832422(NtCPS)

NSY:	104217342 104217889

NTO:	104117658 104121515

NAU:	109211878 109217158

INI:	109158259(CPS1)

ITR:

116015103

SIND:

105163273 105165295

OEU:	111384641 111411797

EGT:	105953222 105954081 105954116 105954117 105975169 105975177 105975321 105975322

SSPL:

121753378 121757143 121762885 121785344 121802217

SMIL:

131008214 131015105 131015110 131016246 131025133

SHIS:

125190513 125200575 125212702 125219486 125223885 125224056

APAN:

127248712 127255778 127258781 127259428

HAN:	110884117 110910286 110922746 110928585

ECAD:

122583086 122589008 122597253 122607123 122609194

LSV:

111886441

CCAV:

112504426 112504645 112518094 112518210

DCR:	108215027 108221652

CSIN:

114314501

RVL:	131314400 131314402 131314403 131323816

AEW:	130762562 130790514 130791486

BVG:

104898566

SOE:

110797142

CQI:	110703048 110704325

ATRI:

130813689

MOF:	131155345 131157652 131157684

NNU:	104596661 104611948

MING:

122071256 122085101 122092041

TSS:	122643769 122648925 122668330

PSOM:

113350098 113350926

OSA:	4329013 9266189

DOSA:

Os02t0278700-01(Os02g0278700) Os02t0571100-01(Os02g0571100)

OBR:

102712336

OGL:	127761380 127763326

BDI:

100825198

ATS:	109734065 109734071 109737607

TDC:	119273021 119303875 119331265 119333090 119338716 119340453 119365260

TAES:

100286847 123057210 123089307 123107348 123149797 123149798 123160464 123166683 123171483

TUA:	125520986 125524309 125547194 125551712

LPER:

127294967 127294979

LRD:	124646644 124649063 124650454 124650524

SBI:

8064919

ZMA:	542165(an2) 542253(an1)

SITA:

101783621 101784049

SVS:	117837121 117840955

PVIR:

120651058 120689545

PHAI:

112877796

PDA:	103697749 103698066 103698242 103699834 103699922 120105661

EGU:

105042778

MUS:	103995767 103996438

ZOF:	121972022 121977045 122051412 122055681

DCT:

110096881

PEQ:

110024537

AOF:

109832398

MSIN:

131222599

NCOL:

116255368

ATR:

18428889

MGR:

MGG_01949

PPEI:

PpBr36_10643

PGRI:

PgNI_12055 PgNI_12444

FMU:	J7337_005030

PCHM:

VFPPC_09474

BZE:	COCCADRAFT_113213

BSC:	COCSADRAFT_104215

BOR:	COCMIDRAFT_30863

» show all

Reference

1 [PMID:4331199]

Authors

Fall RR, West CA.

Title

Purification and properties of kaurene synthetase from Fusarium moniliforme.

Journal

J Biol Chem 246:6913-28 (1971)

Reference

2 [PMID:7994182]

Authors

Sun TP, Kamiya Y

Title

The Arabidopsis GA1 locus encodes the cyclase ent-kaurene synthetase A of gibberellin biosynthesis.

Journal

Plant Cell 6:1509-18 (1994)
DOI:10.1105/tpc.6.10.1509

Sequence

[ath:AT4G02780]

Reference

3 [PMID:9268298]

Authors

Kawaide H, Imai R, Sassa T, Kamiya Y

Title

Ent-kaurene synthase from the fungus Phaeosphaeria sp. L487. cDNA isolation, characterization, and bacterial expression of a bifunctional diterpene cyclase in fungal gibberellin biosynthesis.

Journal

J Biol Chem 272:21706-12 (1997)
DOI:10.1074/jbc.272.35.21706

Sequence

[ag:BAA22426]

Reference

4 [PMID:10803977]

Authors

Toyomasu T, Kawaide H, Ishizaki A, Shinoda S, Otsuka M, Mitsuhashi W, Sassa T

Title

Cloning of a full-length cDNA encoding ent-kaurene synthase from Gibberella fujikuroi: functional analysis of a bifunctional diterpene cyclase.

Journal

Biosci Biotechnol Biochem 64:660-4 (2000)
DOI:10.1271/bbb.64.660