Entry
Name
tRNAPhe (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase;
TYW5;
tRNA yW-synthesizing enzyme 5
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
tRNAPhe 7-(3-amino-3-carboxypropyl)wyosine37,2-oxoglutarate:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
7-(3-amino-3-carboxypropyl)wyosine37 in tRNAPhe + 2-oxoglutarate + O2 = 7-(2-hydroxy-3-amino-3-carboxypropyl)wyosine37 in tRNAPhe + succinate + CO2 [RN:
R10522 ]
Reaction(KEGG)
Substrate
7-(3-amino-3-carboxypropyl)wyosine37 in tRNAPhe;
2-oxoglutarate [CPD:
C00026 ];
O2 [CPD:
C00007 ]
Product
7-(2-hydroxy-3-amino-3-carboxypropyl)wyosine37 in tRNAPhe;
succinate [CPD:
C00042 ];
CO2 [CPD:
C00011 ]
Comment
Requires Fe2+. The enzyme is not active with wybutosine.
History
EC 1.14.11.42 created 2013
Orthology
K18066 tRNA wybutosine-synthesizing protein 5
Genes
PLEU : 114688994 114704683(Tyw5)
MORG : 121444845 121458923(Tyw5)
DNM : 101430963 101446969(TYW5)
CCAR : 109050426 109095722 109095952
CAUA : 113053273 113098509 113108616
CGIB : 127964522 128019798
PPRL : 129357075 129374582(tyw5)
HVI : 124355467 124356036 124356037
PMEO : 129597602 129601728
DPOL : 127846998 127848146
AMIL : 114947417 114949337
OLU : OSTLU_34749 OSTLU_42544
MPP : MICPUCDRAFT_58151(JGI:MicpuC2_58151)
GTT : GUITHDRAFT_143741 GUITHDRAFT_158760
» show all
Taxonomy
Reference
Authors
Noma A, Ishitani R, Kato M, Nagao A, Nureki O, Suzuki T
Title
Expanding role of the jumonji C domain as an RNA hydroxylase.
Journal
Sequence
Reference
Authors
Kato M, Araiso Y, Noma A, Nagao A, Suzuki T, Ishitani R, Nureki O
Title
Crystal structure of a novel JmjC-domain-containing protein, TYW5, involved in tRNA modification.
Journal
Sequence
Other DBs
LinkDB
All DBs