KEGG ENZYME: 1.14.11.57

ENZYME: 1.14.11.57

Entry

EC 1.14.11.57               Enzyme

Name

L-proline trans-4-hydroxylase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

Sysname

L-proline,2-oxoglutarate:oxygen oxidoreductase (trans-4-hydroxylating)

Reaction(IUBMB)

L-proline + 2-oxoglutarate + O2 = trans-4-hydroxy-L-proline + succinate + CO2 [RN:R01252]

Reaction(KEGG)

R01252

Substrate

L-proline [CPD:C00148];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]

Product

trans-4-hydroxy-L-proline [CPD:C01157];
succinate [CPD:C00042];
CO2 [CPD:C00011]

Comment

Requires Fe2+ and ascorbate. The enzyme, isolated from multiple bacterial species, only produces trans-4-hydroxy-L-proline (cf. EC 1.14.11.56, L-proline cis-4-hydroxylase).

History

EC 1.14.11.57 created 2017

Orthology

K21615

L-proline 4-hydroxylase

Genes

PAO:	Pat9b_4264

TVD:	SG34_031150

PLZ:	S4054249_22495

PSEN:

PNC201_00730

AMAA:

amad1_21538

AMAL:

I607_20252

AMAE:

I876_01340

AMAO:

I634_01440

AMAI:

I635_21544

MICZ:

GL2_08620

PLEI:

Q9312_19085

BSTG:

WT74_26330

BANN:

JFN94_20280

PKF:	RW095_03875 RW095_04240 RW095_08395 RW095_22025

BGM:	CAL15_21815

PUS:	CKA81_15295

AMIM:

MIM_c00990(ectD1)

ODI:

ODI_R4011

PACR:

FXN63_04590 FXN63_24425

POL:

Bpro_1714

POS:	DT070_00515

POO:	F7R28_08935

PVAC:

HC248_03375(ectD)

VAP:	Vapar_3472

VPE:	Varpa_3986

VPD:	VAPA_1c35800

VBO:	CKY39_22560

VAM:	C4F17_08670

RTA:

Rta_12710

LIH:	L63ED372_01407

ATER:

MW290_03440

HHT:	F506_06170

HFR:	G5S34_08675

MNR:	ACZ75_23950

MASY:

DPH57_25030

EHB:	E7V67_021955

GLC:	JQN73_19600

ETB:	N7L95_29615

URU:	DSM104443_02581

UPL:	DSM104440_02316

BEBA:

BWI17_13935

BJA:	blr7814(blr7814)

BJU:	BJ6T_15260

BJP:	RN69_07540

BRA:

BRADO6432

BBT:

BBta_1202

BRS:

S23_63740

AOL:

S58_65510

BRC:	BCCGELA001_05070

BRAD:

BF49_3241

BIC:	LMTR13_34430

BRO:	BRAD285_6340

BRK:	CWS35_11670

BOT:	CIT37_16680

BRQ:	CIT40_02930

BGQ:	X265_32840

BGZ:	XH91_31265

BSYM:

CIT39_01345

BBET:

F8237_11225

BARH:

WN72_43850

BVZ:	BRAD3257_0945

BEL:	BE61_18570

BDG:	LPJ38_09370

BSEP:

HAP48_0009920

BQB:	J4P68_0014530

BSEI:

KMZ68_04785

BBAN:

J4G43_007145

BCAN:

BcanWSM471_33930

BAUT:

QA635_06080

BBRA:

QA636_05960

BGK:	IC762_31440

BCOU:

IC761_32845

BPAH:

QA639_05640

BXN:	I3J27_04825

BROS:

QUH67_31195

BRAZ:

LRP30_04145

VGO:	GJW-30_1_01504

PSIN:

CAK95_08080

RBM:

TEF_17475

SIW:	GH266_15005

SMAG:

AN936_03180

SMAZ:

LH19_18060

BLAS:

BSY18_3438

BFW:	B5J99_07980

ACR:

Acry_1558

AMV:	ACMV_16040

SHUM:

STHU_48060

SVC:	STVA_48240

STEL:

STAQ_43390

SKT:	IGS68_12620

SMUC:

JL100_012130

SROE:

JL101_012435

TXI:

TH3_14860

TLU:	R1T41_02375

THAR:

T8K17_09780

SNEA:

NBZ79_09035

SCU:	SCE1572_09855

KPUL:

GXN76_09075

SFA:

Sfla_0103

SBH:

SBI_06435

SDV:	BN159_8034

STRP:

F750_6922

SVT:	SVTN_03385

SLD:

T261_7676

SLC:	SL103_22590

SCZ:	ABE83_21650

SGS:	AVL59_00145

SKY:	D0C37_30525

STRO:

STRMOE7_33575

SFK:

KY5_3455

SGD:	ELQ87_34790 ELQ87_34805

SVN:	CP980_27835

SHAW:

CEB94_33310

SRK:	FGW37_01365

SVR:	CP971_11460

SRIM:

CP984_39860

SSPB:

CP982_01020 CP982_32360

SPLA:

CP981_13870

SCYG:

S1361_20280

SCIR:

STRCI_000636

SDRZ:

NEH16_31810

SARG:

HKX69_08235

SHAU:

K9S39_20800

SCHG:

NRO40_26865

SINN:

ABB07_24050

KAU:	B6264_06540

KAL:	KALB_2186 KALB_2595 KALB_4743

SAQ:

Sare_4561

MCRA:

ID554_12490

AIH:	Aiant_17850

ACTR:

Asp14428_49150

CEP:	Cri9333_0762 Cri9333_1598

NED:	HUN01_07850

AVA:

Ava_C0003

AWA:	AA650_17245

ANN:	EH233_17360

CALN:

NIES2098_11580

DOU:	BMF77_01760(ectD)

STOQ:

K2F26_16905

VAB:

WPS_16830

MTAR:

DF168_01191(ectD_6)

CPI:

Cpin_5186

FLW:	LVD16_11040

ALUT:

O5O44_10400

» show all

Reference

1 [PMID:8546682]

Authors

Lawrence CC, Sobey WJ, Field RA, Baldwin JE, Schofield CJ

Title

Purification and initial characterization of proline 4-hydroxylase from Streptomyces griseoviridus P8648: a 2-oxoacid, ferrous-dependent dioxygenase involved in etamycin biosynthesis.

Journal

Biochem J 313 ( Pt 1):185-91 (1996)
DOI:10.1042/bj3130185

Reference

2 [PMID:10473412]

Authors

Shibasaki T, Mori H, Chiba S, Ozaki A

Title

Microbial proline 4-hydroxylase screening and gene cloning.

Journal

Appl Environ Microbiol 65:4028-31 (1999)
DOI:10.1128/AEM.65.9.4028-4031.1999

Sequence

[ag:BAA20094]