Entry
Name
feruloyl-CoA 6-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
feruloyl-CoA,2-oxoglutarate:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
trans-feruloyl-CoA + 2-oxoglutarate + O2 = trans-6-hydroxyferuloyl-CoA + succinate + CO2 [RN:
R11549 ]
Reaction(KEGG)
Substrate
Product
Comment
Requires iron(II) and ascorbate. The product spontaneously undergoes trans-cis isomerization and lactonization to form scopoletin, liberating CoA in the process. The enzymes from the plants Ruta graveolens and Ipomoea batatas also act on trans-4-coumaroyl-CoA. cf. EC
1.14.11.62 , trans-4-coumaroyl-CoA 2-hydroxylase.
History
EC 1.14.11.61 created 2019
Pathway
ec00999 Biosynthesis of various plant secondary metabolites
ec01110 Biosynthesis of secondary metabolites
Orthology
K06892 feruloyl-CoA 6-hydroxylase
K23378 feruloyl-CoA 6-hydroxylase / trans-4-coumaroyl-CoA 2-hydroxylase
Genes
CSAT : 104742969 104745611 104765146 104778096
BRP : 103842722 103859525 103870109
BNA : 106345852 106351698 106387021 106395905 106440607 106452295
BOE : 106326584 106334207 106343388
RSZ : 108805589 108807602 108856971 108860815 130512442
THJ : 104810332 104811207 104822670
CIT : 102611531 102611821 102612308 102613413
CIC : CICLE_v10015700mg CICLE_v10015715mg CICLE_v10017524mg CICLE_v10017914mg CICLE_v10018321mg CICLE_v10018343mg CICLE_v10029948mg
MINC : 123208605 123208607
GRA : 105781174 105781207 105781216 105782099 105792686 105793736 105799341
GHI : 107891457 107909062 107911341 107911342 107912965 107919558 107919564 107943772 107945535 121204738 121204739 121204740
GAB : 108453468 108457145 108463405 108463460 108463463 108469299 108469339 108469473
HSYR : 120121434 120123198 120123217 120129179 120142046 120144265 120144266
DZI : 111277730 111277731 111293995 111294252 111294332 111294334 111294335 111303902 111304032
GMX : 100527581 100777556 100820326(F6'H1)
GSJ : 114397605 114406404 114418863
PVU : PHAVU_006G023200g PHAVU_006G023300g PHAVU_010G036100g
CCAJ : 109807179 109818965
APRC : 113854989 113855375 113856319
TPRA : 123906175 123910291
LJA : Lj3g3v0652730.1(Lj3g3v0652730.1) Lj6g3v1007880.2(Lj6g3v1007880.2)
AHF : 112717830 112775183 112777938
PCIN : 129290037 129303332
QSA : O6P43_003828 O6P43_020930 O6P43_020932
PMUM : 103318655 103331402
ZJU : 107410478 107415011 107415012 107415053 107415055 125421938 132803448
MNT : 112090580 112091386 112091846 112092533 112092542 112093530 112095114 21412228 21412376
CSAV : 115710001 115719386
RCU : 8282975 8282976 8282977 8282978
JCU : 105631594 105631595 105631596 105631723 105637198
HBR : 110642752 110642753 110642754 110642760 110646506 110649460 110652136 110653324 110653328
MESC : 110618463 110619142 110619678 110625121 110625127 110625140 110625159
PEU : 105115498 105142340 105142343
PALZ : 118027905 118027908 118055560
CILL : 122292889 122294945
CAVE : 132164422 132164540
QSU : 111987312 111991061 111991086 112010742 112032284 112032285 112032288
QLO : 115975208 115975298 115975380 115975550 115975625 115975752 115981013 115981027 115982079 115994543
TWL : 119980108 119983035 120014269 120014278
SLY : 101261369 101262487 101265061 101266300 101266599 101266903
SPEN : 107004343 107004359 107004745 107004747 107029794
SOT : 102586708 102588955 102589603 102589939 102590254 102590589 102590939 102596149 102602524 107058105 107058705 107058802
SSTN : 125845134 125845135 125845136 125845137 125845138 125845701 125846948 125850401 125868939 125869050 125869070 125873869 125873870
SDUL : 129884319 129885041 129904128
CANN : 107848279 107848763
LBB : 132600751 132600753 132604627 132604630 132617624 132623405 132628413 132629292 132629402
NTA : 107763182 107770966 107784295 107785648 107787977 107788458 107796566 107803881 107810699
NSY : 104211447 104215338 104215878 104215879 104222156 104245046
NTO : 104085679 104085681 104086193 104106751 104106843 104106844 104111996
NAU : 109226105 109228288 109232742 109232743 109235784 109239160 109239161
INI : 109152302 109152304 109152305 109152306 109152436 109152437 109159769 109160283 109160284 109160285 109160335 109160336 109192759 109192940
ITR : 116003962 116004157 116004252 116005043 116014693 116014694 116014695 116024801 116024932 116025013 116025703 116025820 116026105 116026336
OEU : 111401066 111405833 111407362 111412305
HAN : 110887178 110890716 110898740 110901341 110903006 110903519 110927168
ECAD : 122582825 122585276 122585606 122598353
LSV : 111882077 111882078 111890219 111919843 111919866 111919868 111919869
CCAV : 112520303 112522132
DCR : 108198298 108198303 108199747 108226415
SOE : 110782747 110790461 110792289 110804615
CQI : 110715086 110715111 110715271 110716896 110724886 110730548 110730632
ATRI : 130815127 130823945
» show all
Taxonomy
Reference
Authors
Kai K, Mizutani M, Kawamura N, Yamamoto R, Tamai M, Yamaguchi H, Sakata K, Shimizu B
Title
Scopoletin is biosynthesized via ortho-hydroxylation of feruloyl CoA by a 2-oxoglutarate-dependent dioxygenase in Arabidopsis thaliana.
Journal
Sequence
Reference
Authors
Bayoumi SA, Rowan MG, Blagbrough IS, Beeching JR
Title
Biosynthesis of scopoletin and scopolin in cassava roots during post-harvest physiological deterioration: the E-Z-isomerisation stage.
Journal
Reference
Authors
Vialart G, Hehn A, Olry A, Ito K, Krieger C, Larbat R, Paris C, Shimizu B, Sugimoto Y, Mizutani M, Bourgaud F
Title
A 2-oxoglutarate-dependent dioxygenase from Ruta graveolens L. exhibits p-coumaroyl CoA 2'-hydroxylase activity (C2'H): a missing step in the synthesis of umbelliferone in plants.
Journal
Reference
Authors
Matsumoto S, Mizutani M, Sakata K, Shimizu B
Title
Molecular cloning and functional analysis of the ortho-hydroxylases of p-coumaroyl coenzyme A/feruloyl coenzyme A involved in formation of umbelliferone and scopoletin in sweet potato, Ipomoea batatas (L.) Lam.
Journal
Sequence
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