KEGG   ENZYME: 1.14.13.218
Entry
EC 1.14.13.218              Enzyme                                 
Name
5-methylphenazine-1-carboxylate 1-monooxygenase;
phzS (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
5-methylphenazine-1-carboxylate,NADH:oxygen oxidoreductase (1-hydroxylating, decarboxylating)
Reaction(IUBMB)
5-methylphenazine-1-carboxylate + NADH + O2 = pyocyanin + NAD+ + CO2 + H2O [RN:R11346]
Reaction(KEGG)
R11346;
(other) R11578
Substrate
5-methylphenazine-1-carboxylate [CPD:C21295];
NADH [CPD:C00004];
O2 [CPD:C00007]
Product
pyocyanin [CPD:C01748];
NAD+ [CPD:C00003];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Pseudomonas aeruginosa, is involved in the biosynthesis of pyocyanin, a toxin produced and secreted by the organism. It can also act on phenazine-1-carboxylate, converting it into phenazin-1-ol.
History
EC 1.14.13.218 created 2016
Pathway
ec00405  Phenazine biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20940  5-methylphenazine-1-carboxylate 1-monooxygenase
Genes
ROR: RORB6_06595
RPLN: B1209_13685
RAO: DSD31_13210
YRE: HEC60_05120
PSGC: G163CM_02640(phzS)
EBF: D782_1281
PAO: Pat9b_1855
PPHO: CTZ24_09760
LAB: LA76x_3342(phzS)
LAQ: GLA29479_2375(phzS)
PAE: PA4217(phzS)
PAEV: N297_4349
PAEI: N296_4349
PAU: PA14_09400(phzS)
PAP: PSPA7_0881
PAG: PLES_07101(phzS)
PAF: PAM18_0720(phzS)
PNC: NCGM2_5462(phzS)
PAEB: NCGM1900_0742(phzS)
PDK: PADK2_03585
PSG: G655_03620
PRP: M062_22220
PAEP: PA1S_03735
PAER: PA1R_gp2108
PAEM: U769_03765
PAEL: T223_03665
PAES: SCV20265_0744
PAEU: BN889_04688(mhbM)
PAEG: AI22_00175
PAEC: M802_4347
PAEO: M801_4215
PPAA: B7D75_03660
GAI: IMCC3135_15925(xlnD)
WOC: BA177_13885
MME: Marme_1535
MPC: Mar181_2201
MARL: HH196_03030
RNC: GO999_24170
RSC: RCFBP_20884
RSL: RPSI07_2763
RSN: RSPO_c02774
RSY: RSUY_26660
RSG: JK151_05465
RPI: Rpic_0514
RPF: Rpic12D_0501
RPJ: N234_02860
RMN: TK49_07090
RIN: ACS15_0633
RWE: KOL96_10650
REH: H16_A0578(h16_A0578)
CNC: CNE_1c06050
CUH: BJN34_03480
CTI: RALTA_A0534
CBW: RR42_m0670
CGD: CR3_2101(ubiH)
CCUP: BKK81_05525 BKK81_20090
CUP: BKK80_04830 BKK80_21715
CUU: BKK79_07450 BKK79_26880
CPAU: EHF44_22900
COX: E0W60_13510
CCAM: M5D45_26530
CCAX: KZ686_15255
CUK: KB879_03295
CNAN: A2G96_04050
BMA: BMAA0391
BMV: BMASAVP1_1585
BML: BMA10229_1769
BMN: BMA10247_A0439
BMAL: DM55_3356
BMAE: DM78_4291
BMAQ: DM76_4054
BMAI: DM57_4923
BMAF: DM51_4113
BMAZ: BM44_3316
BMAB: BM45_4154
BPS: BPSS2111
BPM: BURPS1710b_A1214
BPL: BURPS1106A_A2849
BPD: BURPS668_A2974
BPSE: BDL_5573
BPSM: BBQ_4004(phzS)
BPSU: BBN_5593(phzS)
BPSD: BBX_4740(phzS)
BPZ: BP1026B_II2272
BPQ: BPC006_II2811
BPK: BBK_4877(phzS)
BPSH: DR55_5621
BPSA: BBU_3886(phzS)
BPSO: X996_5401
BUT: X994_4948
BSAV: WS86_29635
BCEN: DM39_5111
BUZ: AYM40_21510
PBON: QS306_14280
PPAI: E1956_36370
PPUL: RO07_14100
PSPU: NA29_23010
PAPI: SG18_13925
PVE: UC34_05365
POX: MB84_09755
PFG: AB870_16335
PNR: AT302_01670
PFIB: PI93_023035
PCOM: NTU39_06225
BPT: Bpet0515 Bpet4332
BBRO: BAU06_23000
BFZ: BAU07_23145
BOH: AKI39_21985
BGM: CAL15_01535
BGV: CAL12_25200
ASW: CVS48_28980
ACHB: DVB37_05300
ACHO: H4P35_05310
AMUI: PE062_02330
PUT: PT7_0833
PUD: G9Q38_01140 G9Q38_02970 G9Q38_10125
PUS: CKA81_04285
POLL: OEG81_08045
PIG: EGT29_07110 EGT29_22735 EGT29_28210
PARK: LSG25_16400
RHF: EUB48_12265
RHOB: HTY51_04350 HTY51_09025
POL: Bpro_2199
ACK: C380_22685
ACRA: BSY15_1202
ACIO: EAG14_01270
ARAD: KI609_21425
AAV: Aave_0794
AAA: Acav_0721
DAC: Daci_3698 Daci_4386
DEL: DelCs14_2432
DTS: BI380_04640 BI380_07740
DHK: BO996_16805 BO996_19770
DLA: I6G47_02380 I6G47_05635
VAP: Vapar_5387
VPE: Varpa_0285
VPD: VAPA_1c03380
VBO: CKY39_01595
VAM: C4F17_17395
RTA: Rta_23930
LIM: L103DPR2_01284(xlnD_2)
HYR: BSY239_2040 BSY239_2625
HYB: Q5W_04140
HCZ: G9Q37_10945
STHM: IS481_16100
RDP: RD2015_403
PKT: AT984_17755
MIU: ABE85_21150
RGU: A4W93_14405
UND: UNDKW_5855
THK: CCZ27_01430
MLN: A9174_07680
MCI: Mesci_2836
MCIC: A4R28_13475
MOP: Mesop_0529
MESW: A9K65_015455
MESM: EJ066_25025
MESP: C1M53_21380
MESO: BSQ44_20480
MES: Meso_2927
AAK: AA2016_0349
AMIS: Amn_03980
NIY: FQ775_05345 FQ775_14565
KMN: HW532_11120
ACUC: KZ699_24685
NGL: RG1141_PA12940
NGG: RG540_PA13930
NEO: CYG48_01500
NPM: QEO92_28650
PDES: FE840_017780
HOE: IMCC20628_02267
OAN: Oant_3996
OAH: DR92_4259
OCR: HGK82_22735
OCL: GTN27_15175
BJA: bll2502(bll2502)
BJU: BJ6T_36290 BJ6T_73250
BJP: RN69_17595 RN69_35515
BRA: BRADO0592
BBT: BBta_2684 BBta_7587
BRS: S23_21960 S23_54840
AOL: S58_52740 S58_70480
BRC: BCCGELA001_25810 BCCGELA001_27265
BRAD: BF49_2290 BF49_5554
BIC: LMTR13_07380 LMTR13_29995
BRO: BRAD285_0059 BRAD285_5019
BRK: CWS35_14465 CWS35_19400
BOT: CIT37_00105 CIT37_23885
BRQ: CIT40_06980 CIT40_22375
BGQ: X265_12385 X265_28550
BGZ: XH91_11155 XH91_26510
BSYM: CIT39_05770 CIT39_21570
BBET: F8237_07380 F8237_26055
BARH: WN72_12725 WN72_33960
BVZ: BRAD3257_1807 BRAD3257_3050 BRAD3257_6551
BEL: BE61_30130
BDG: LPJ38_15290 LPJ38_32215
BSEP: HAP48_0006725
BQB: J4P68_0029205
BSEI: KMZ68_07695 KMZ68_19895
BBAN: J4G43_013775 J4G43_036435
BCAN: BcanWSM471_11595 BcanWSM471_26895
BAUT: QA635_10550
BBRA: QA636_09635
BGK: IC762_08755
BCOU: IC761_11345 IC761_26760
BPAH: QA639_11310
BXN: I3J27_08715 I3J27_26380
BROS: QUH67_08775 QUH67_27280
BRAZ: LRP30_10440 LRP30_27950
RPA: TX73_018595
RPB: RPB_1945
RPT: Rpal_4108
RPX: Rpdx1_1762
RBK: E0H22_08280 E0H22_20405
VGO: GJW-30_1_00854(xlnD_1)
TRB: HB776_01665 HB776_28510
TALZ: RPMA_05725 RPMA_08725
BVV: BHK69_12440 BHK69_29720
BVES: QO058_29305
BOP: AXW83_01690
BOS: BSY19_1787
BOI: BLM15_12140 BLM15_12830
BOF: FQV39_18055
LNE: FZC33_04360
MET: M446_3637
MNO: Mnod_1206
MAQU: Maq22A_c15030(ubiH)
MEE: DA075_24315
MTEA: DK419_08600
MIND: mvi_06480
CHEL: AL346_06160
RHZ: RHPLAN_29510 RHPLAN_38520 RHPLAN_41260 RHPLAN_49870
PEM: OF122_14585
DEI: C4375_06665
BRN: D1F64_21235
PSIN: CAK95_08390 CAK95_09835
NOH: G5V57_23915
RBM: TEF_16205
PPHR: APZ00_18290
SIW: GH266_11530 GH266_16295
PHR: C6569_06010 C6569_12535
PSTG: E8M01_22865 E8M01_33480
PAQT: E8L99_12810 E8L99_15705
SIL: SPO0105
SIT: TM1040_3441
PGA: PGA1_c32020
PGL: PGA2_c00680
PGD: Gal_00046
PHP: PhaeoP97_00080
PPIC: PhaeoP14_00046
PHQ: D1820_13510
LMD: METH_18965
LEJ: ETW24_18490
LAQU: R2C4_19230
LEV: ETW23_21320
LCAE: K3721_16725
PABY: Ga0080574_TMP326
SUAM: BOO69_14545
SULI: C1J05_00215 C1J05_15695
SPOT: G6548_14995
SMED: JNX03_12645
SUAL: KDD17_04110
SPSE: SULPSESMR1_00290(hpxO)
RID: RIdsm_02011(xlnD)
AHT: ANTHELSMS3_04104
SAGU: CDO87_15335
THAA: CFI11_23685
OCD: FHY55_04475
ROT: FIV09_19090(xlnD2)
RMH: LVO79_13065
MALU: KU6B_16810
TGL: HFZ77_15830
PALX: GQA70_14325
LALG: LentiSH36_01379
TRY: QF118_09155
RMAI: MACH21_01800
PARS: DRW48_00150
PSAP: JHX88_11230
PTP: RCA23_c26230
RMB: K529_017940
TSCO: R1T40_17255
SALO: EF888_02425
SEDI: EBB79_00110
PPSO: QPJ95_07060
BOO: E2K80_06690
PSEB: EOK75_00130
PPRU: FDP22_18945
PAMO: BAR1_08670
POZ: I0K15_14380
PGV: SL003B_3095
THAS: C6Y53_14570
HDH: G5B40_10800
NSM: JO391_00355 JO391_17975
AMYL: QBD29_01885
NAR: Saro_2238
NOT: C7W88_14160
NDR: HT578_01140
NHUM: PQ457_21550
SWI: Swit_3061
SPHD: HY78_10270
SECH: B18_04230
PMEA: KTC28_18680
ACR: Acry_0321
AMV: ACMV_03520
ROS: CTJ15_00570
RMUC: FOB66_02020
RFL: Rmf_32600
SRF: LHU95_07625
FER: FNB15_15520
THAR: T8K17_06075
TMO: TMO_3126(mhbM)
APB: SAR116_0061
SNEA: NBZ79_10020
SCL: sce4807 sce4897 sce7525
SCU: SCE1572_07320 SCE1572_19460 SCE1572_24720 SCE1572_28650
BKW: BkAM31D_08555(xlnD_1)
VIK: KFZ58_14395
LAO: AOX59_16860
NDT: L1999_16820
NCM: QNK12_17745
BCHS: JNE38_15130
MSA: Mycsm_03029
MYV: G155_17420
MDX: BTO20_14290
MSM: MSMEG_3841
MSG: MSMEI_3751
MSB: LJ00_19080
MSN: LI99_19085
MSH: LI98_19090
MNE: D174_17475
MYN: MyAD_17130
MFT: XA26_35530
MAUU: NCTC10437_03211(xlnD_1)
MHEV: MHEL_55810
MAUB: MAUB_10010
MPHU: MPHO_03200
MMUC: C1S78_016830
MMAT: MMAGJ_70400
MBOK: MBOE_38750
MFG: K6L26_14510
MSEI: MSEDJ_44470
MGO: AFA91_20850
MSEN: K3U95_14820
MAB: MAB_2237c
MMV: MYCMA_1195
MABB: MASS_2167
MABL: MMASJCM_2192
MCHE: BB28_11255
MIZ: BAB75_11975
MSAO: MYCSP_09625
MSAL: DSM43276_01950(xlnD_2)
MVM: MJO54_10270
CBOV: CBOVI_07110(hpxO)
NCY: NOCYR_0786
NNO: NONO_c08530
NAH: F5544_05600 F5544_12955
NWL: NWFMUON74_45100
RANT: RHODO2019_18205
SVL: Strvi_5052
SHY: SHJG_0206 SHJG_0216 SHJG_0800
SHO: SHJGH_0633
SRC: M271_47475
SMAL: SMALA_0139
SSOI: I1A49_04125
SBH: SBI_01059
SVE: SVEN_5103
SGU: SGLAU_04905
SVT: SVTN_26265
SLD: T261_1426 T261_7434
SLC: SL103_08090
SXI: SXIM_24700 SXIM_24770
STRC: AA958_29950 AA958_30740
SPAV: Spa2297_23920 Spa2297_23950
SCLF: BB341_29775
SALW: CP975_12045
STRD: NI25_24410
SNW: BBN63_05605 BBN63_09595
SAUO: BV401_04430
SALF: SMD44_02958
SLX: SLAV_02135
STRO: STRMOE7_02870 STRMOE7_28970 STRMOE7_29000
SFK: KY5_2781c
SLK: SLUN_27535
SQZ: FQU76_24165 FQU76_30760
SKA: CP970_28680
SGZ: C0216_14055
SVN: CP980_31315 CP980_32485
SFIC: EIZ62_09255
SPHV: F9278_42105
STSU: B7R87_23900 B7R87_26355
SGX: H4W23_27835
SSPB: CP982_16040 CP982_37385
SHUN: DWB77_06919(xlnD)
SAUH: SU9_000240
SMOB: J7W19_26555 J7W19_31275
SHK: J2N69_12195
SLF: JEQ17_47520
SAOV: G3H79_24680
SDUR: M4V62_27495
STAA: LDH80_13045
STUD: STRTU_006378
SANT: QR300_40355
SOV: QZH56_32905
SFP: QUY26_26010
SCHG: NRO40_20990 NRO40_23305
SCT: SCAT_p0890
SCY: SCATT_p08430
KAB: B7C62_28850 B7C62_28880
YIA: LO772_03595
MICR: BMW26_14970
MFOL: DXT68_14210
MPRT: ET475_07395
MAZA: NFX31_00765
MELY: L2X98_30450
MSUW: GCM10025863_27010 GCM10025863_27020
MVD: AWU67_12410 AWU67_14070
AGY: ATC03_03885
AARC: G127AT_14310
AMAU: DSM26151_05520(phzS)
ASOI: MTP13_07185
ALAV: MTO99_14110
LEU: Leucomu_09990
LTR: EVS81_13845
LLUT: K1X41_09870
LALL: MUN78_04895
LRP: MUN76_11620
LTN: KVY00_14280
AWW: G8758_05090
RTER: IDM49_02300
DAY: FV141_06960
DERM: H7F30_10455
LMOI: VV02_01275
IDO: I598_2461(hpxO_3)
CFL: Cfla_0298
CEZ: CBP52_12505
CPAL: F1D97_01910
CDON: KKR89_01650
CWAN: KG103_01690
CWN: NP075_01760
CXIE: NP048_01755
GYU: FE374_11800
NDA: Ndas_5023
NAKE: KGD83_26685
AMAZ: LUW76_05595
ACIR: OG979_31730
SPIQ: OHA34_10475 OHA34_10485
ACTQ: OG417_33960 OG417_37755
SRO: Sros_6172
NOA: BKM31_03465 BKM31_19720 BKM31_22685 BKM31_35635 BKM31_48365
NOW: GBF35_47245
NCX: Nocox_08730(xlnD1)
NGN: LCN96_09765 LCN96_24140 LCN96_38550
NFS: OIE67_14140
MHAI: OHB01_22695 OHB01_30820
FRE: Franean1_5376
AMYC: CU254_21595
AMYY: YIM_22825(xlnD1)
AORI: SD37_26575
PDX: Psed_3194
PSEE: FRP1_21735
PSEQ: AD006_00910
PECQ: AD017_08735
PAUT: Pdca_23520
KBU: Q4V64_08325
PMAD: BAY61_11550
MICB: MicB006_2976
MTUA: CSH63_12410
MCHL: PVK74_09905
ACTR: Asp14428_17800
ASIC: Q0Z83_058760
ATL: Athai_51270
ASER: Asera_23600
CAI: Caci_4355
SNA: Snas_3295
AYM: YM304_31330
DMR: Deima_0219
PGIN: FRZ67_05915
HHY: Halhy_0467
SLI: Slin_0336
SMON: AWR27_00625
STAE: HNV11_08740
AANH: G9X63_04575
ALEN: G9X62_06085
EOL: Emtol_1587
RSI: Runsl_0519
RUN: DR864_05530
RUP: DTQ70_10575
CLIT: OQ292_22630
 » show all
Reference
1  [PMID:11591691]
  Authors
Mavrodi DV, Bonsall RF, Delaney SM, Soule MJ, Phillips G, Thomashow LS
  Title
Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1.
  Journal
J Bacteriol 183:6454-65 (2001)
DOI:10.1128/JB.183.21.6454-6465.2001
  Sequence
[pae:PA4217]
Reference
2  [PMID:17253782]
  Authors
Parsons JF, Greenhagen BT, Shi K, Calabrese K, Robinson H, Ladner JE
  Title
Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa.
  Journal
Biochemistry 46:1821-8 (2007)
DOI:10.1021/bi6024403
Reference
3  [PMID:18416536]
  Authors
Greenhagen BT, Shi K, Robinson H, Gamage S, Bera AK, Ladner JE, Parsons JF
  Title
Crystal structure of the pyocyanin biosynthetic protein PhzS.
  Journal
Biochemistry 47:5281-9 (2008)
DOI:10.1021/bi702480t
  Sequence
[pae:PA4217]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.218
IUBMB Enzyme Nomenclature: 1.14.13.218
ExPASy - ENZYME nomenclature database: 1.14.13.218
BRENDA, the Enzyme Database: 1.14.13.218
LinkDB

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