KEGG   ENZYME: 1.14.13.59
Entry
EC 1.14.13.59               Enzyme                                 
Name
L-lysine N6-monooxygenase (NADPH);
lysine N6-hydroxylase;
L-lysine 6-monooxygenase (NADPH) (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
L-lysine,NADPH:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
L-lysine + NADPH + H+ + O2 = N6-hydroxy-L-lysine + NADP+ + H2O [RN:R00448]
Reaction(KEGG)
R00448
Substrate
L-lysine [CPD:C00047];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
N6-hydroxy-L-lysine [CPD:C01028];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A flavoprotein (FAD). The enzyme from strain EN 222 of Escherichia coli is highly specific for L-lysine; L-ornithine and L-homolysine are, for example, not substrates.
History
EC 1.14.13.59 created 1999, modified 2001, modified 2012
Pathway
ec00997  Biosynthesis of various other secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01120  Microbial metabolism in diverse environments
Orthology
K03897  lysine N6-hydroxylase
Genes
EOI: ECO111_1296(iucD)
EOJ: ECO26_1348(iucD)
ECOO: ECRM13514_1293(iucD)
ECOH: ECRM13516_1249(iucD)
ESL: O3K_23070
ESO: O3O_02285
ESM: O3M_22985
ECK: EC55989_4642(iucD)
ENA: ECNA114_4698(iucD)
ECOS: EC958_3274(iucD)
ECV: APECO1_O1CoBM169(iucD)
ECM: EcSMS35_3179(iucD)
ECQ: ECED1_4902(iucD)
EUM: ECUMN_3372(iucD)
ECT: ECIAI39_3426(iucD)
EOC: CE10_3449(iucD)
ECZ: ECS88_p0010(iucD)
ECC: c3624(iucD)
ELN: NRG857_30237(iucD)
EAB: ECABU_c32820(iucD)
ELC: i14_3317(iucD)
ELD: i02_3317(iucD)
ECOJ: P423_16375
ERUY: OSH18_13360(iucD)
SEM: STMDT12_C38540
SES: SARI_02155
SFL: SF3718(iucD)
SFX: S4053(iucD)
SFV: SFV_3851(iucD)
SFE: SFxv_4051
SFN: SFy_5297
SFS: SFyv_5371
SFT: NCTC1_04015(iucD)
SSN: SSON_3604(iucD)
SBO: SBO_4340(iucD)
SBC: SbBS512_E4631(iucD)
ENC: ECL_03319(iucD)
ENL: A3UG_14870
ECLG: EC036_28790
ECLE: ECNIH2_14835
ECLN: ECNIH4_08320
ECLI: ECNIH5_13755
ECLX: LI66_14275
ECLZ: LI64_13685
EHM: AB284_11220
EXF: BFV63_14020
ECLA: ECNIH3_13810
ECLC: ECR091_13745
EAU: DI57_04915
EKB: BFV64_14425
ENO: ECENHK_14315
EEC: EcWSU1_02922(iucD)
ELG: BH714_10665
ERN: BFV67_13935
ECLS: LI67_011575
ECHG: FY206_15845
ESH: C1N69_14700
EPT: HWQ17_21545
ENX: NI40_014285
LNI: CWR52_20770
EBG: FAI37_14855
END: A4308_19000
ENZ: G0034_14905
ENS: HWQ15_22485
ENK: LOC22_02265
EMOR: L6Y89_13945
ENB: ELK40_14625
EQU: OM418_14010
EPU: QVH39_14955
ESA: ESA_pESA3p05550
CSZ: CSSP291_21033
CSJ: CSK29544_1p0026
CCON: AFK62_20065
CDM: AFK67_21005
CSI: P262_p1011
CMJ: AFK66_020210
CUI: AFK65_20165
CTU: Ctu_1p01070(iucD)
KPU: KP1_p315(iucD)
KPO: KPN2242_25266
KPR: KPR_4894(iucD)
KPNK: BN49_pII0065(iucD)
KMI: VW41_08305
CKO: CKO_00970
CNT: JT31_04210
CEM: LH23_13810
CEN: LH86_13120
CLAP: NCTC11466_02784(iucD)
YRE: HEC60_04360
TOE: QMG90_12575
YPE: YPO0993(iucD)
YPK: y3384(iucD)
YPH: YPC_0799(iucD)
YPA: YPA_0271
YPN: YPN_3191
YPM: YP_3447(iucD)
YPP: YPDSF_0525
YPZ: YPZ3_0745(iucD)
YPT: A1122_00010
YPD: YPD4_0700(iucD)
YPX: YPD8_0700(iucD)
YPW: CH59_870(iucD)
YPJ: CH55_3547(iucD)
YPV: BZ15_2580(iucD)
YPL: CH46_4152(iucD)
YPS: YPTB3266(iucD)
YPO: BZ17_3344(iucD)
YPI: YpsIP31758_0711(iucD)
YPY: YPK_0783
YPB: YPTS_3402
YPQ: DJ40_3171(iucD)
YPU: BZ21_2546(iucD)
YPR: BZ20_2848(iucD)
YPC: BZ23_2827(iucD)
YPF: BZ19_2672
YSI: BF17_03350
YIN: CH53_2434(iucD)
YAK: ACZ76_16795
YMA: DA391_18850
YMO: HRD69_17565
SMAR: SM39_3651 SM39_4454(iucD)
SMAC: SMDB11_3458 SMDB11_4148(iucD)
SMW: SMWW4_v1c41520
SPE: Spro_0025 Spro_0919 Spro_2520
SRR: SerAS9_0844 SerAS9_2495
SRL: SOD_c07750(iucD1) SOD_c23600(iucD2) SOD_c47380(iucD)
SRY: M621_04275 M621_12890 M621_25650
SPLY: Q5A_004125(iucD_1) Q5A_013040(iucD_2)
SRS: SerAS12_0844 SerAS12_2496
SRA: SerAS13_0844 SerAS13_2497
SMAF: D781_0884
SLQ: M495_03880 M495_12465 M495_24705
SERF: L085_07285
SERS: SERRSCBI_00130 SERRSCBI_20200
SFW: WN53_13455
SFG: AV650_09005
SRZ: AXX16_4217
SERM: CLM71_04285
SQU: E4343_03620 E4343_11905 E4343_16150
SFJ: SAMEA4384070_0951(iucD)
SOF: NCTC11214_00581(iucD)
SSUR: ATE40_017480
SURI: J0X03_03140
SRHZ: FO014_05495
SENP: KHA73_04105 KHA73_23070
SNEM: NLX84_21450
SNEV: OI978_10090
RAH: Rahaq_3478
RACE: JHW33_11610
RAQ: Rahaq2_3554
RAA: Q7S_17510
ROX: BV494_12445
RVC: J9880_03080
RBON: QNM34_17835
RIU: I2123_19285
FSM: CCS41_02975
EAME: GXP68_03935
RBAD: H2866_12030
RCB: O1V66_13555
PWA: Pecwa_0950
PPAR: A8F97_14180
PEC: W5S_0841
PCAC: OI450_13395(iucD)
DDA: Dd703_3091
BRB: EH207_14280
LBQ: CKQ53_00780
LPOP: I6N93_03520
ETO: RIN69_18765
EGE: EM595_p0529(iucD)
PAM: PANA_0353(iucD)
PLF: PANA5342_4061(iucD)
PAJ: PAJ_3508(iucD)
PAQ: PAGR_g3924
PSTW: DSJ_03460
KPIE: N5580_21625
TATU: EFZ10_15515
VCY: IX92_17060
VCT: JV59_21965
VSI: MTO69_17220
VMI: AL543_14495
VSH: BSZ05_19030
VKA: BTD91_15885
VOS: KNV97_12545
VTI: CEQ48_09235
VMT: QYQ96_04685
VFI: VF_A0164(iucD)
VFM: VFMJ11_A0200
VSA: VSAL_II0278(iucD)
GHO: AL542_16535
SALY: E8E00_15545
SCOT: HBA18_15435
SPRO: N7E60_14040
PST: PSPTO_0211(iucD)
PAVL: BKM03_01095
PVR: PverR02_14275
PSK: U771_15470
PXN: HU772_013785
PMUY: KSS95_16080
PAGR: E2H98_15805
KAK: Kalk_08725
SWD: Swoo_0586
PAEW: KIH87_11625
AGQ: LQZ07_20620
MMAA: FR932_14170
SPOI: IMCC21906_00377
MICT: FIU95_16360(iucD)
MICZ: GL2_22490(iucD)
MVB: MJO52_16665
ASIP: AQUSIP_25460(iucD)
LPO: LPO_2065(iucD)
LPF: lpl1946
LHA: LHA_1351(iucD)
LSH: CAB17_06275
LCJ: NCTC11976_01019(iucD)
MISZ: MishRS11D_43100(iucD)
NOC: Noc_1811
NWA: Nwat_1301
HTX: EKK97_08780
KUS: B9G99_09250
KMA: B9H00_05600
KUY: FY550_02650
PAUR: FGL86_03615
SAJS: QO259_04305
EEI: NX720_08185
ENG: O2T12_00705
KKO: Kkor_2040
KGE: TQ33_0707
CHRO: CXB49_00465
CRZ: D1345_06150
CHAE: CH06BL_13350
MAER: DAI18_18595
BCJ: BCAS0043
BCEO: I35_7041
BCED: DM42_6412
BSTL: BBJ41_34310
BARI: NLX30_34745
BURK: DM992_33275
BPY: Bphyt_0280
BCAI: K788_0006333
PSPW: BJG93_16680
PBRY: NDK50_34985
OUR: CEQ07_01965
CPRA: CPter91_0284
MANC: IV454_24120
MLO: mlr5923
MLN: A9174_32405
MCI: Mesci_5807
MCIC: A4R28_21890
MOP: Mesop_6383
MAM: Mesau_05856
MAMO: A6B35_31120
MESW: A9K65_031345
MESM: EJ066_12270
MESP: C1M53_02775
MJR: EB229_33125
MERD: EB233_30775
MOH: IHQ72_34065
MMEI: LRP31_09865
AMIS: Amn_11140
PHT: BLM14_29930
PHYL: HB779_01090
KMN: HW532_05380
AVV: RvVAT039_40620
AVF: RvVAR031_33170
AVI: Avi_5731
RET: RHE_PF00456(vbsO)
REC: RHECIAT_PC0000681(vbsO)
REL: REMIM1_PF00420(iucD)
REP: IE4803_PC00508(iucD)
REI: IE4771_PD00518(iucD)
RLE: pRL120314(vbsO)
RLT: Rleg2_4809
RLB: RLEG3_07065
RLU: RLEG12_10340
RTR: RTCIAT899_PC05660
RHN: AMJ98_PC00365 AMJ98_PE00534(iucD)
RPHA: AMC79_PC00336 AMC79_PD00672(iucD)
RHX: AMK02_PC00354 AMK02_PD00403(iucD)
RHK: Kim5_PA00462 Kim5_PC00465(iucD)
REZ: AMJ99_PB00359 AMJ99_PD00534(iucD)
RHR: CKA34_19735
RAD: CO657_27950
RII: FFM53_025065
RHID: FFM81_025100
RBQ: J2J99_23410
RLN: J0663_24075
RBAN: J2J98_22760
RAW: NE851_32990
RLW: RlegWSM1455_25315
ARA: Arad_8229(vbsO)
RBW: RLCC275e_24815
OAN: Oant_3254
OAH: DR92_3235(iucD)
OCR: HGK82_18830
OCL: GTN27_19035
BJA: blr2131(blr2131)
BJU: BJ6T_77190
BJP: RN69_37485
BRC: BCCGELA001_30200
BGZ: XH91_07535
BEL: BE61_77460(iucD)
BSEP: HAP48_0005940
BBAN: J4G43_043230
BAUT: QA635_32100
BBRA: QA636_33715
BXN: I3J27_33210
BVV: BHK69_19665
AZC: AZC_3247
LNE: FZC33_08125
MET: M446_5059
METS: DK389_06835
MSL: Msil_3318
HDI: HDIA_3128(iucD)
PPHR: APZ00_04765
CON: TQ29_06785
THAA: CFI11_19950
PFIS: JHX87_10370
POZ: I0K15_07675
GBE: GbCGDNIH1_2375
GBH: GbCGDNIH2_2375
GBC: GbCGDNIH3_2375
GBS: GbCGDNIH4_2375
TXI: TH3_13065
THAI: IT893_15040
CCRO: CMC5_023430
NANN: O0S08_38350
BBA: Bd1573
BBAT: Bdt_1566
BBAC: EP01_04085
BDZ: DOM22_13135
BDK: HW988_12135
BMX: BMS_0525(iucD)
HAX: BALOs_0964(iucD)
BSTO: C0V70_12470
PSTI: SOO65_15530
NSL: BOX37_20165
NTP: CRH09_08345
NOZ: DMB37_12225
NAH: F5544_07265
RHA: RHA1_ro08654
ROA: Pd630_LPD03701
SANL: KZO11_35730
SGRF: SGFS_066720
SCI: B446_31510
STRE: GZL_09053
SLD: T261_7621
SLC: SL103_05005
STSI: A4E84_35470
SNR: SNOUR_40995
SPUN: BFF78_29745
SGV: B1H19_09125
SLX: SLAV_39955(iucD3)
STRO: STRMOE7_14925
SGE: DWG14_06029(iucD_2)
SQZ: FQU76_11675
SVR: CP971_29835
STSU: B7R87_11320 B7R87_30270
SMOB: J7W19_03315
SGJ: IAG43_25475
SINE: KI385_07805
SYUN: MOV08_42040
SJN: RI060_04845
SHAU: K9S39_10135
RIA: C7V51_07215
KFL: Kfla_2932
KQI: F1D05_35110
FRA: Francci3_3275
FSY: FsymDg_2883
SESP: BN6_51340(iucD)
KBU: Q4V64_41730
SAQ: Sare_0571 Sare_1046
MAU: Micau_3806
MIL: ML5_4610
MICB: MicB006_2881
MTUA: CSH63_06350
MCHL: PVK74_09360
MPRN: Q3V37_03980
PLK: CIK06_17640
DVC: Dvina_35560 Dvina_40770
DMAT: Dmats_30405 Dmats_35660
CAI: Caci_6320
NAV: JQS30_08325
FNE: FSDG_01143
FOE: JJC03_16525
KAN: IMCC3317_12960(iucD)
 » show all
Reference
1  [PMID:2518519]
  Authors
Plattner HJ, Pfefferle P, Romaguera A, Waschutza S, Diekmann H.
  Title
Isolation and some properties of lysine N6-hydroxylase from Escherichia coli strain EN222.
  Journal
Biol Met 2:1-5 (1989)
DOI:10.1007/bf01116193
Reference
2  [PMID:8504838]
  Authors
Macheroux P, Plattner HJ, Romaguera A, Diekmann H.
  Title
FAD and substrate analogs as probes for lysine N6-hydroxylase from Escherichia coli EN 222.
  Journal
Eur J Biochem 213:995-1002 (1993)
DOI:10.1111/j.1432-1033.1993.tb17846.x
Reference
3  [PMID:8218389]
  Authors
Thariath AM, Fatum KL, Valvano MA, Viswanatha T.
  Title
Physico-chemical characterization of a recombinant cytoplasmic form of lysine: N6-hydroxylase.
  Journal
Biochim Biophys Acta 1203:27-35 (1993)
DOI:10.1016/0167-4838(93)90032-M
Reference
4  [PMID:2935523]
  Authors
de Lorenzo V, Bindereif A, Paw BH, Neilands JB
  Title
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12.
  Journal
J Bacteriol 165:570-8 (1986)
DOI:10.1128/JB.165.2.570-578.1986
  Sequence
[ag:AAA23196]
Reference
5
  Authors
Marrone, L., Siemann, S., Beecroft, M. and Viswanatha, T.
  Title
Specificity of lysine:N-6-hydroxylase: A hypothesis for a reactive substrate intermediate in the catalytic mechanism.
  Journal
Bioorg Chem 24:401-406 (1996)
Reference
6  [PMID:2493814]
  Authors
Goh CJ, Szczepan EW, Menhart N, Viswanatha T.
  Title
Studies on lysine:N6-hydroxylation by cell-free systems of Aerobacter aerogenes 62-1.
  Journal
Biochim Biophys Acta 990:240-5 (1989)
DOI:10.1016/S0304-4165(89)80040-6
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.59
IUBMB Enzyme Nomenclature: 1.14.13.59
ExPASy - ENZYME nomenclature database: 1.14.13.59
BRENDA, the Enzyme Database: 1.14.13.59
CAS: 64295-82-5
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