KEGG ENZYME: 1.14.14.83

ENZYME: 1.14.14.83

Entry

EC 1.14.14.83               Enzyme

Name

geraniol 8-hydroxylase;
CYP76B6 (gene name);
G10H (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

geraniol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (8-hydroxylating)

Reaction(IUBMB)

geraniol + [reduced NADPH---hemoprotein reductase] + O2 = (6E)-8-hydroxygeraniol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R08785]

Reaction(KEGG)

R08785

Substrate

geraniol [CPD:C01500];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]

Product

(6E)-8-hydroxygeraniol [CPD:C17621];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]

Comment

A cytochrome P-450 (heme thiolate) protein found in plants. Also hydroxylates nerol and citronellol, cf. EC 1.14.14.84, linalool 8-monooxygenase. The recommended numbering of geraniol gives 8-hydroxygeraniol as the product rather than 10-hydroxygeraniol as used by references 1-3. See prenol nomenclature {iupac/misc/prenol#p1::Pr-1}. The cloned enzyme also catalysed, but less efficiently, the 3'-hydroxylation of naringenin (cf. EC 1.14.14.82, flavonoid 3'-monooxygenase) [3].

History

EC 1.14.14.83 created 2012 as EC 1.14.13.152, transferred 2018 to EC 1.14.14.83

Pathway

ec00902

Monoterpenoid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K15099

geraniol 8-hydroxylase