Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and the other dehydrogenated
hapalindole U [CPD:C21973];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007];
chloride [CPD:C00698];
12-epi-fischerindole U [CPD:C21840]
Product
hapalindole G [CPD:C21974];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001];
12-epi-fischerindole G [CPD:C21975]
Comment
The enzyme, characterized from hapalindole-type alkaloids-producing cyanobacteria, is a specialized iron(II)/2-oxoglutarate-dependent oxygenase that catalyses the chlorination of its substrates in a reaction that requires oxygen, chloride ions, iron(II) and 2-oxoglutarate.
Discovery of a Promiscuous Non-Heme Iron Halogenase in Ambiguine Alkaloid Biogenesis: Implication for an Evolvable Enzyme Family for Late-Stage Halogenation of Aliphatic Carbons in Small Molecules.