Entry
Name
2-methylene-furan-3-one reductase;
FaEO;
SIEO;
enone oxidoreductase;
4-hydroxy-2,5-dimethylfuran-3(2H)-one:NAD(P)+ oxidoreductase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
4-hydroxy-2,5-dimethylfuran-3(2H)-one:NADP+ oxidoreductase
Reaction(IUBMB)
4-hydroxy-2,5-dimethylfuran-3(2H)-one + NADP+ = 4-hydroxy-5-methyl-2-methylenefuran-3(2H)-one + NADPH + H+ [RN:
R10593 ]
Reaction(KEGG)
Substrate
4-hydroxy-2,5-dimethylfuran-3(2H)-one [CPD:
C20717 ];
NADP+ [CPD:
C00006 ]
Product
4-hydroxy-5-methyl-2-methylenefuran-3(2H)-one [CPD:
C20718 ];
NADPH [CPD:
C00005 ];
H+ [CPD:
C00080 ]
Comment
The enzyme was dicovered in strawberry (Fragaria x ananassa), where it produces furaneol, one of the major aroma compounds in the fruits. It has also been detected in tomato (Solanum lycopersicum) and pineapple (Ananas comosus). The enzyme can also act on derivatives substituted at the methylene functional group. The enzyme from the yeast Saccharomyces cerevisiae acts on (2E)-2-ethylidene-4-hydroxy-5-methylfuran-3(2H)-one and produces homofuraneol, an important aroma compound in soy sauce and miso. NADPH is the preferred cofactor.
History
EC 1.3.1.105 created 2013
Orthology
K18980 2-methylene-furan-3-one reductase
Genes
CSAT : 104741071 104756736 104776495
CIT : 102609064 102613872 102620685
CIC : CICLE_v10024569mg CICLE_v10032327mg
MINC : 123207383 123213878 123217669
TCC : 108660475 18600752 18600753 18600754 18609461
GRA : 105771822 105771829 105779846 105794655 105798314
GHI : 107886583 107888641 107892290 107909666 107926100 107926450 107926451 107956898 107959846
GAB : 108459352 108474757 108475250 108486047 108488666
HSYR : 120123646 120125379 120193035 120212897 120215265
DZI : 111287470 111299579 111303397 111315926 111315927 111317864
EGR : 104414223 104419265 104419266 104419267 104419268 104419269 104419270 104419271 104419272 104444318 120288292
GMX : 100780585 100788619 100791274 100792857 100793908 100810816 100817947
GSJ : 114390622 114397499 114398480 114398948 114400546 114400547 114423155
PVU : PHAVU_003G060300g PHAVU_004G014100g PHAVU_004G014200g PHAVU_008G151800g
VRA : 106758097 106763614 106766249 106766978 106767067 106779866
VAR : 108326926 108333536 108344801 108345091 108346133
VUN : 114176659 114181449 114181459 114182354 114182581 114182582 114185889
VUM : 124822100 124836000 124836728 124836730 124843394 124843395
CCAJ : 109788795 109788798 109788825 109788828 109788830 109790901 109791793 109791795 109791796 109793948 109809560
APRC : 113857906 113861532 113867538 113867582
MTR : 11412290 11412408 11412983 11416757 120575844 25481654 25498003 25501340
TPRA : 123889184 123897285 123898032 123906558 123906568 123907775 123914442 123921481
CAM : 101488474 101499001 101502075 101505613 101510459
PSAT : 127075091 127075798 127127885 127127966 127127994
VVO : 131611460 131616705 131616707 131617200 131618406 131636525 131646111
LJA : Lj0g3v0339299.1(Lj0g3v0339299.1) Lj1g3v3641820.1(Lj1g3v3641820.1) Lj1g3v3641840.1(Lj1g3v3641840.1) Lj2g3v1014110.1(Lj2g3v1014110.1) Lj3g3v2722620.1(Lj3g3v2722620.1) Lj5g3v0642790.1(Lj5g3v0642790.1) Lj5g3v0642800.1(Lj5g3v0642800.1)
ADU : 107462129 107462133 107481322 107490758 107490781 107490782 107490783 107491997 107495049
AIP : 107605332 107631315 107641036 107642048 107644032 107644033 107644034 107645753 107648495
AHF : 112695373 112697315 112733912 112747168 112750800 112750801 112750803 112755221 112755222 112759165 112791794 112802459 112802460 112802461 112802463 112805865
LANG : 109332827 109337116 109341016 109341017 109361183
PCIN : 129291702 129312002 129315301 129316864 129321662
QSA : O6P43_004615 O6P43_008968 O6P43_008969 O6P43_008970 O6P43_008971 O6P43_008972 O6P43_008973 O6P43_008974 O6P43_008975 O6P43_008977 O6P43_008978 O6P43_027120 O6P43_032188 O6P43_033160
FVE : 101294385(EO) 101308862
RCN : 112186062 112186063 112186065 112188200 112196038
PPER : 18782356 18782358 18782540 18783184 18783430 18792497
PMUM : 103321761 103329864 103329865 103329866 103329867 103329868 103329869 103330173 103330175
PAVI : 110753197 110753839 110753936 110754254 110754353
PDUL : 117622572 117622798 117623602 117623604 117634111
MDM : 103403010 103411163 103411164 103442256 103442258 103451965
MSYL : 126583556 126583557 126583559 126596070 126607411
ZJU : 107415383 107415384 107415385 107415400 107415405 107423748 125419867 125420016 125421968 132803598
MNT : 21403391 21403392 21403393 21403394 21403395 21410382
CSAV : 115699695 115714925 115717507
MCHA : 111006288 111019164
CMAX : 111470369 111487421 111489564
CMOS : 111444796 111458186 111462548
CPEP : 111803230 111803257 111808811
HBR : 110638856 110656252 110657526
MESC : 110605038 110629066
PALZ : 118035608 118056812
JRE : 108986779 108992833 108992834 108992835 108992854 109006930 109010331
CILL : 122286530 122302580 122303131 122314371
CAVE : 132177844 132180656 132180657 132180697 132187488
QSU : 111998050 112007152 112008871 112028837 112033741
QLO : 115958245 115976669 115978215 115978280 115982584
NTA : 107761424 107766097 107811890 107821492
INI : 109155963 109166812 109178321 109178322
SIND : 105156605 105168259 105174244
OEU : 111395273 111395443 111406335 111406336 111406337 111406881 111410814
EGT : 105963730 105964829 105974736
SSPL : 121753937 121753987 121758202 121759469 121767536 121798247
SMIL : 131023689 131025288 131025291
SHIS : 125203855 125205242 125219207 125219467
APAN : 127245597 127256168 127260392
HAN : 110871690 110871693 110871694 110911641
ECAD : 122579387 122604131
CCAV : 112525373 112525542 112527490
DCR : 108199585 108204433 108206041 108206261 108218859
CSIN : 114275063 114276601 114297010 114310679
RVL : 131298886 131298887 131301805 131310280 131334028
AEW : 130771821 130783010 130785440
CQI : 110690925 110694161 110707678 110708694
MOF : 131144196 131166333 131166335
MING : 122075670 122086541
PSOM : 113276441 113282405
DOSA : Os08t0379400-01(Os08g0379400)
TAES : 123132361 123136413 123143922
PVIR : 120678014 120713662
ZOF : 121992407 121996354 121996355
DCT : 110093333 110093345 110093351
PEQ : 110033692 110033694 110033695
MSIN : 131228026 131233123 131237731
SMO : SELMODRAFT_76590 SELMODRAFT_95183
CCP : CHC_T00002111001 CHC_T00002112001
» show all
Taxonomy
Reference
Authors
Raab T, Lopez-Raez JA, Klein D, Caballero JL, Moyano E, Schwab W, Munoz-Blanco J
Title
FaQR, required for the biosynthesis of the strawberry flavor compound 4-hydroxy-2,5-dimethyl-3(2H)-furanone, encodes an enone oxidoreductase.
Journal
Sequence
Reference
Authors
Klein D, Fink B, Arold B, Eisenreich W, Schwab W
Title
Functional characterization of enone oxidoreductases from strawberry and tomato fruit.
Journal
Reference
Authors
Schiefner A, Sinz Q, Neumaier I, Schwab W, Skerra A
Title
Structural basis for the enzymatic formation of the key strawberry flavor compound 4-hydroxy-2,5-dimethyl-3(2H)-furanone.
Journal
Reference
Authors
Uehara K, Watanabe J, Mogi Y, Tsukioka Y
Title
Identification and characterization of an enzyme involved in the biosynthesis of the 4-hydroxy-2(or 5)-ethyl-5(or 2)-methyl-3(2H)-furanone in yeast.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 1.3.1.105
LinkDB
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