KEGG   ENZYME: 1.4.2.2
Entry
EC 1.4.2.2                  Enzyme                                 
Name
nicotine dehydrogenase;
nicA2 (gene name)
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With a cytochrome as acceptor
Sysname
(S)-nicotine:cytochrome c oxidoreductase (N-methylmimosine-forming)
Reaction(IUBMB)
(S)-nicotine + 2 ferricytochrome c = N-methylmyosmine + 2 ferrocytochrome c + 2 H+ [RN:R11175]
Reaction(KEGG)
R11175
Substrate
(S)-nicotine [CPD:C00745];
ferricytochrome c [CPD:C00125]
Product
N-methylmyosmine [CPD:C21154];
ferrocytochrome c [CPD:C00126];
H+ [CPD:C00080]
Comment
The enzyme, characterized from the bacterium Pseudomonas putida S16, contains an FAD cofactor and belongs to the flavin-containing amine oxidase family. The enzyme from this bacterium is specific for the c-type cytochrome CycN. The product undergoes spontaneous hydrolysis to form pseudooxynicotine.
History
EC 1.4.2.2 created 2022
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K20169  nicotine dehydrogenase
Genes
PPT: PPS_4081
PMON: X969_19995
PMOT: X970_19630
PMED: E3Z27_20810
PVK: EPZ47_21960
PSJY: AA098_04100 AA098_21435
SLAA: EUU25_06970
Reference
1  [PMID:24204321]
  Authors
Tang H, Wang L, Wang W, Yu H, Zhang K, Yao Y, Xu P
  Title
Systematic unraveling of the unsolved pathway of nicotine degradation in Pseudomonas.
  Journal
PLoS Genet 9:e1003923 (2013)
DOI:10.1371/journal.pgen.1003923
  Sequence
[ppt:PPS_4081]
Reference
2  [PMID:33432238]
  Authors
Dulchavsky M, Clark CT, Bardwell JCA, Stull F.
  Title
A cytochrome c is the natural electron acceptor for nicotine oxidoreductase.
  Journal
Nat Chem Biol 17:344-350 (2021)
DOI:10.1038/s41589-020-00712-3
  Sequence
[ppt:PPS_4081]
Other DBs
ExplorEnz - The Enzyme Database: 1.4.2.2
IUBMB Enzyme Nomenclature: 1.4.2.2
ExPASy - ENZYME nomenclature database: 1.4.2.2
BRENDA, the Enzyme Database: 1.4.2.2
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