ENZYME: 2.1.1.324 Help
Entry Name dTDP-4-amino-2,3,4,6-tetradeoxy-D-glucose N,N-dimethyltransferase;
Class Transferases;BRITE hierarchy
Sysname S-adenosyl-L-methionine:dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose N,N-dimethyltransferase
Reaction(IUBMB) 2 S-adenosyl-L-methionine + dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose = 2 S-adenosyl-L-homocysteine + dTDP-alpha-D-forosamine (overall reaction) [RN:
R08932 ];
(1a) S-adenosyl-L-methionine + dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose = S-adenosyl-L-homocysteine + dTDP-4-(methylamino)-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose [RN:
R11452 ];
(1b) S-adenosyl-L-methionine + dTDP-4-(methylamino)-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose = S-adenosyl-L-homocysteine + dTDP-alpha-D-forosamine [RN:
R11453 ]
Reaction(KEGG) Substrate S-adenosyl-L-methionine [CPD:
C00019 ];
dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose [CPD:
C18033 ];
dTDP-4-(methylamino)-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose [CPD:
C21395 ]
Product S-adenosyl-L-homocysteine [CPD:
C00021 ];
dTDP-alpha-D-forosamine [CPD:
C18034 ];
dTDP-4-(methylamino)-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose [CPD:
C21395 ]
Comment The enzyme was isolated from the bacterium Saccharopolyspora spinosa, where it is involved in the biosynthesis of spinosyn A, an active ingredient of several commercial insecticides.
History EC 2.1.1.324 created 2016
Pathway ec00523 Polyketide sugar unit biosynthesis
ec01110 Biosynthesis of secondary metabolites
Orthology K13330 dTDP-4-amino-2,3,4,6-tetradeoxy-D-glucose N,N-dimethyltransferase
Genes Taxonomy Reference Authors Hong L, Zhao Z, Melancon CE 3rd, Zhang H, Liu HW
Title In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa.
Journal Other DBs ExPASy - ENZYME nomenclature database: 2.1.1.324
LinkDB All DBs
