KEGG ENZYME: 2.1.1.349

ENZYME: 2.1.1.349

Entry

EC 2.1.1.349                Enzyme

Name

toxoflavin synthase;
toxA (gene name)

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:1,6-didemethyltoxoflavin N1,N6-dimethyltransferase (toxoflavin-forming)

Reaction(IUBMB)

(1) S-adenosyl-L-methionine + 1,6-didemethyltoxoflavin = S-adenosyl-L-homocysteine + reumycin [RN:R12157];
(2) S-adenosyl-L-methionine + reumycin = S-adenosyl-L-homocysteine + toxoflavin [RN:R12158]

Reaction(KEGG)

R12157 R12158

Substrate

S-adenosyl-L-methionine [CPD:C00019];
1,6-didemethyltoxoflavin [CPD:C21956];
reumycin [CPD:C21957]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
reumycin [CPD:C21957];
toxoflavine [CPD:C16789]

Comment

The enzyme is a dual-specificity methyltransferase that catalyses the last two steps of toxoflavin biosynthesis. Toxoflavin is a major virulence factor of several bacterial crop pathogens.

History

EC 2.1.1.349 created 2018

Orthology

K20331

toxoflavin synthase

Genes

SYM:	K6K13_17790

YAK:	ACZ76_13200 ACZ76_16160

SFJ:	SAMEA4384070_2573

SOF:	NCTC11214_04378(bioC_2)

PLU:

plu0250

PLUM:

A4R40_01225

PAY:

PAU_03241

PTT:	VY86_11790

PAKH:

B0X70_01085

PLUI:

CE143_01085

XBO:	XBJ1_1516 XBJ1_2779

XBV:	XBW1_0361 XBW1_0364 XBW1_3521

XNE:	XNC1_1384 XNC1_2019 XNC1_2445

XNM:	XNC2_1346 XNC2_1954 XNC2_2352

XDO:	XDD1_1102(toxA) XDD1_1316 XDD1_1320 XDD1_1508

XPO:	XPG1_2381 XPG1_2700

XHO:	A9255_09690 A9255_16450

XBU:	HGO23_08785 HGO23_13520

XGR:	QL128_13470

PALA:

CO695_02920

BUJ:	BurJV3_3707

STEN:

CCR98_18885

STEQ:

ICJ04_13845

LAB:	LA76x_0657

LAQ:	GLA29479_2850

LCP:	LC55x_4965

LEZ:

GLE_0529

LYA:	RDV84_03775

VNP:	KW548_07795

PMOL:

CLJ08_02150

PMOS:

O165_012485

PFL:

PFL_1036

PPRC:

PFLCHA0_c10560(toxA)

PPRO:

PPC_1074

PMUD:

NCTC8068_03529(toxA)

PLQ:	AA042_15235

PPV:	NJ69_03265

PSEC:

CCOS191_2151

PYM:	AK972_5317

PDW:

BV82_3620

PXN:	HU772_015150

PMUY:

KSS95_17865

PMAM:

KSS90_10405

PTK:	EXN22_17150

PTAE:

NCTC10697_01384(toxA)

MCA:

MCA0484

MMEO:

OOT43_09550

CYS:	NYP54_04445

TTI:	THITH_10570

HCS:	FF32_13340

HVN:	EI420_07645

ADI:

B5T_03651

ENW:	MJO57_09165

ENG:	O2T12_06780

BDL:	AK34_1842 AK34_3761

BGL:	bglu_2g06400

BGU:

KS03_5278

BGD:	bgla_1g04570

BGO:

BM43_1844

BIC:	LMTR13_24470

BRAZ:

LRP30_33270

MSC:

BN69_1794

MBRY:

B1812_13435

MROS:

EHO51_00310

AZZ:	DEW08_24275

SNEA:

NBZ79_02900

MSD:	MYSTI_02000 MYSTI_05830

MFB:	MFUL124B02_06660 MFUL124B02_28840

CCX:	COCOR_03369(ubiG4) COCOR_05983

SUR:	STAUR_6832

AGE:	AA314_07139

AVM:	JQX13_49480

MBD:	MEBOL_006373 MEBOL_006914

CFUS:

CYFUS_003503 CYFUS_005489 CYFUS_008555

SCU:	SCE1572_20315

CCRO:

CMC5_053700(ubiE) CMC5_062770(smtA) CMC5_062800(smtA)

PAUU:

E8A73_009980 E8A73_016780

MRM:	A7982_08219 A7982_13440

MUL:

MUL_2739

MMI:	MMAR_3525 MMAR_3636 MMAR_4425

MMAE:

MMARE11_34420 MMARE11_35440 MMARE11_42500

MLI:	MULP_03780

MPSE:

MPSD_36050 MPSD_45780

MSHO:

MSHO_02170 MSHO_49470

MSPG:

F6B93_11885 F6B93_14415

NBR:	O3I_015605

NTP:	CRH09_33970

RHW:	BFN03_03385

PDEF:

P9209_25820

SVE:

SVEN_3943

SDV:	BN159_1463

SALS:

SLNWT_7298

SVT:	SVTN_29985

STRE:

GZL_00343

SLD:

T261_1553

SLC:	SL103_16730

STRM:

M444_06095 M444_32570 M444_36500

SRW:	TUE45_01220(ubiG_1)

SGS:	AVL59_28695

SALU:

DC74_8060

SALL:

SAZ_41625

SGV:	B1H19_33505

SALF:

SMD44_00437

SALJ:

SMD11_0158 SMD11_6408 SMD11_6799

SLX:	SLAV_32705

STRO:

STRMOE7_02825

SFK:

KY5_0115

SKA:	CP970_42325

SGZ:	C0216_16250 C0216_18680

SVN:	CP980_29715 CP980_30410

SRK:	FGW37_30550 FGW37_30570 FGW37_30610

SFIC:

EIZ62_09530 EIZ62_31840

SVR:	CP971_30390

SAQU:

EJC51_40760

SBY:	H7H31_01780 H7H31_22130

SRIM:

CP984_06170 CP984_29705

SSUB:

CP968_01295 CP968_29700 CP968_29830

SCIN:

CP977_28300

SCHA:

CP983_20860

SGX:	H4W23_13750

SSPB:

CP982_02060 CP982_05925 CP982_28355

SNF:	JYK04_01385(bchM)

SHUN:

DWB77_07165(ubiG_5)

SCYG:

S1361_01915

SFEU:

IM697_31080

SPEU:

CGZ69_35255

SROI:

IAG44_03755

SXN:	IAG42_30705

SACT:

DMT42_14735

SYAN:

NRK68_03720 NRK68_30885

STAA:

LDH80_09125

SNIG:

HEK616_78900

SCIR:

STRCI_007059

SXT:	KPP03845_100538(ind7) KPP03845_101123(ubiE_1)

SLON:

LGI35_04610

SGK:	PET44_04660 PET44_31010 PET44_33940

SHAU:

K9S39_09015 K9S39_11160

SFP:	QUY26_08880

SCHG:

NRO40_24290

STTN:

BSL84_03475 BSL84_30735

STRZ:

OYE22_01790 OYE22_21305

SCT:	SCAT_5482 SCAT_p1624

SCY:	SCATT_54800 SCATT_p01000

KSK:

KSE_73110

KAU:	B6264_23650

ACIR:

OG979_18850

SEN:

SACE_7128

AMYC:

CU254_04900

ARHD:

VSH64_00990

APRE:

CNX65_23095

AMI:

Amir_4628

SESP:

BN6_45550

SSYI:

EKG83_21765

KUT:	JJ691_05120(coq3_1) JJ691_36530(coq3_4)

KPHY:

AOZ06_29455 AOZ06_31565

LED:	BBK82_13205

ACTU:

Actkin_03411

UME:	RM788_05655

PLAB:

C6361_36195

PLAT:

C6W10_33740

SYNE:

Syn6312_3678

CYN:	Cyan7425_0895 Cyan7425_3410

CYC:	PCC7424_0911

OXY:	HCG48_13190

ANB:	ANA_C12771

DHT:	NG743_22140

STOQ:

K2F26_05955

PUO:	RZN69_06215

GMR:	GmarT_49760(novO)

GIM:	F1728_20575

GPN:	Pan110_07630(couO) Pan110_49510(bioC_2)

GFM:	Enr17x_49740(bioC_2)

SDYN:

Mal52_05470(novO)

PVAR:

SH412_001230

SRHO:

HH216_19135

RSI:	Runsl_2309

RUP:	DTQ70_28135

FIB:	A6C57_23755

HTS:	HMJ29_00435

NNEO:

PQG83_16570

» show all

Reference

1 [PMID:27070241]

Authors

Fenwick MK, Philmus B, Begley TP, Ealick SE

Title

Burkholderia glumae ToxA Is a Dual-Specificity Methyltransferase That Catalyzes the Last Two Steps of Toxoflavin Biosynthesis.

Journal

Biochemistry 55:2748-59 (2016)
DOI:10.1021/acs.biochem.6b00167

Sequence

[bgl:bglu_2g06400]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.349

IUBMB Enzyme Nomenclature:

2.1.1.349

ExPASy - ENZYME nomenclature database:

2.1.1.349

BRENDA, the Enzyme Database:

2.1.1.349

LinkDB

DBGET integrated database retrieval system