KEGG   ENZYME: 3.1.1.122
Entry
EC 3.1.1.122                Enzyme                                 
Name
carbendazim hydrolysing esterase;
mheI (gene name)
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
Sysname
carbendazim methanol hydrolase (decarboxylating)
Reaction(IUBMB)
carbendazim + H2O = 2-aminobenzimidazole + CO2 + methanol (overall reaction) [RN:R13238];
(1a) carbendazim + H2O = N-(1H-1,3-benzodiazol-2-yl)carbamate + methanol [RN:R13239];
(1b) N-(1H-1,3-benzodiazol-2-yl)carbamate = 2-aminobenzimidazole + CO2 (spontaneous) [RN:R13240]
Reaction(KEGG)
R13238 R13239 R13240
Substrate
carbendazim [CPD:C10897];
H2O [CPD:C00001];
N-(1H-1,3-benzodiazol-2-yl)carbamate [CPD:C22848]
Product
2-aminobenzimidazole [CPD:C10901];
CO2 [CPD:C00011];
methanol [CPD:C00132];
N-(1H-1,3-benzodiazol-2-yl)carbamate [CPD:C22848]
Comment
The enzyme, which is inducible in the soil bacterium Nocardioides sp. (strain SG-4G), catalyses the degradation of the fungicide carbendazim. Following hydrolysis of the carbamate ester, the carbamate decarboxylates spontaneously.
History
EC 3.1.1.122 created 2023
Orthology
K27193  carbendazim hydrolysing esterase
Reference
1  [PMID:20228105]
  Authors
Pandey G, Dorrian SJ, Russell RJ, Brearley C, Kotsonis S, Oakeshott JG.
  Title
Cloning and biochemical characterization of a novel carbendazim (methyl-1H-benzimidazol-2-ylcarbamate)-hydrolyzing esterase from the newly isolated Nocardioides sp. strain SG-4G and its potential for use in enzymatic bioremediation.
  Journal
Appl Environ Microbiol 76:2940-5 (2010)
DOI:10.1128/AEM.02990-09
  Sequence
[ag:ACV42482]
Other DBs
ExplorEnz - The Enzyme Database: 3.1.1.122
IUBMB Enzyme Nomenclature: 3.1.1.122
ExPASy - ENZYME nomenclature database: 3.1.1.122
BRENDA, the Enzyme Database: 3.1.1.122
CAS: 10605-21-7
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