KEGG ENZYME: 4.2.2.1

ENZYME: 4.2.2.1

Entry

EC 4.2.2.1                  Enzyme

Name

hyaluronate lyase;
hyaluronidase (ambiguous);
glucuronoglycosaminoglycan lyase (ambiguous);
spreading factor;
mucinase (ambiguous)

Class

Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides

Sysname

hyaluronate lyase

Reaction(IUBMB)

Cleaves hyaluronate chains at a beta-D-GlcNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine. [RN:R02465 R06242]

Reaction(KEGG)

R02465 R06242(G)

Comment

The enzyme catalyses the degradation of hyaluronan by a beta-elimination reaction. Also acts on chondroitin. The product is more systematically known as 3-(4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-2-acetamido-2-deoxy-D-glucose

History

EC 4.2.2.1 created 1961 as EC 4.2.99.1, transferred 1972 to EC 4.2.2.1, modified 2001

Orthology

K01727

hyaluronate lyase

Genes

SAU:	SA2003(hysA)

SAV:	SAV2202(hysA)

SAW:	SAHV_2186(hysA)

SAH:	SaurJH1_2271

SAJ:	SaurJH9_2232

SAM:	MW2129(hysA)

SAS:

SAS2103

SAR:	SAR1892(hysA1) SAR2292(hysA2)

SAC:	SACOL2194(hysA)

SAX:	USA300HOU_2197(hysA)

SAA:	SAUSA300_2161(hysA)

SAO:	SAOUHSC_02463

SAE:	NWMN_2106(hysA)

SAD:

SAAV_2263

SUU:	M013TW_2169

SUV:	SAVC_09900

SUE:

SAOV_2247

SUJ:	SAA6159_01731 SAA6159_02114

SUK:	SAA6008_02243(hysA)

SUC:	ECTR2_2063

SUT:	SAT0131_02378

SUQ:	HMPREF0772_10989 HMPREF0772_11345

SUZ:

MS7_2224

SUD:	ST398NM01_1880 ST398NM01_2262

SUX:	SAEMRSA15_21070(hysA2)

SUW:	SATW20_23400(hysA)

SUG:	SAPIG1880 SAPIG2262

SUF:	SARLGA251_16860(hysA) SARLGA251_19960(hysA)

SAUA:

SAAG_00036 SAAG_01706

SAUE:

RSAU_001662(hysA) RSAU_002045(hysA2)

SAUN:

SAKOR_02172

SAUS:

SA40_1956(hysA)

SAUU:

SA957_2040(hysA)

SAUG:

SA268_2111(hysA)

SAUZ:

SAZ172_2305(hysA)

SAUT:

SAI1T1_2016390(hysA)

SAUJ:

SAI2T2_1016400(hysA)

SAUK:

SAI3T3_1016390(hysA)

SAUQ:

SAI4T8_1016400(hysA)

SAUV:

SAI7S6_1016390

SAUW:

SAI5S5_1016330

SAUX:

SAI6T6_1016340

SAUY:

SAI8T7_1016370

SAUF:

X998_2189

SAB:	SAB1663(hysA1) SAB2082(hysA2)

SUY:	SA2981_2139(hysA)

SAUB:

C248_1856(hysA1) C248_2244(hysA2)

SAUM:

BN843_22440

SAUC:

CA347_2289

SAUR:

SABB_04270(hysA)

SAUI:

AZ30_11650

SAUD:

CH52_07940

SAMS:

NI36_10880

SUH:	SAMSHR1132_20330

SHU:	SHYC_11715(hysA)

SAGQ:

EP23_10455

SSH:	NCTC13712_02147(hylB)

SSAC:

I6I31_03920

SRAI:

LN051_02175

LPET:

lgb_01413

LFO:	LMK00_08220

SPY:	SPy_1032(hylA)

SPZ:	M5005_Spy0757(hylA)

SPYM:

M1GAS476_0819(hylA)

SPYA:

A20_0798c

SPM:	spyM18_1014(hylA)

SPG:	SpyM3_0665(hylA)

SPS:

SPs1188

SPH:	MGAS10270_Spy0874(hylA)

SPI:	MGAS10750_Spy0909(hylA)

SPJ:	MGAS2096_Spy0830(hylA)

SPK:	MGAS9429_Spy0871(hylA)

SPA:	M6_Spy0782

SPB:	M28_Spy0736(hylA)

STG:	MGAS15252_0784(hylA)

STX:	MGAS1882_0780(hylA)

SOZ:	Spy49_0811c(hylA)

STZ:	SPYALAB49_000782

SPYH:

L897_03945

SPN:

SP_0314

SPD:

SPD_0287

SPR:	spr0286(hysA)

SPW:

SPCG_0322

SJJ:

SPJ_0310

SNV:	SPNINV200_02820

SPX:	SPG_0285(hyl)

SNT:

SPT_0364

SND:

MYY_0397

SPNN:

T308_01575

SNE:	SPN23F02890

SPV:

SPH_0426

SNC:	HMPREF0837_10617

SNM:	SP70585_0379

SPP:

SPP_0350

SNI:	INV104_02610

SPNG:

HMPREF1038_00364(hysA)

SNB:	SP670_0382

SNP:

SPAP_0347

SNU:	SPNA45_01722

SAG:

SAG1197

SAN:

gbs1270

SAK:	SAK_1284(hylB)

SGC:

A964_1170

SAGS:

SaSA20_1016(hylB)

SAGL:

GBS222_1026(hylB)

SAGM:

BSA_12760

SAGI:

MSA_13200

SAGR:

SAIL_13150

SAGP:

V193_05585

SAGC:

DN94_05585

SAGT:

GBSCOH1_1103

SAGE:

EN72_06630

SAGG:

EN73_06165

SAGJ:

ID870_03375(hylB)

SUP:

YYK_05005

SST:	SSUST3_1226

SSUY:

YB51_6050

SSQ:	SSUD9_1371

SRP:	SSUST1_0748

SSUI:

T15_0747

SEZ:	Sez_1299(hylB)

SEQ:

SZO_06680

SEQU:

Q426_02630

SDS:

SDEG_0654

SDG:	SDE12394_03510

SDA:	GGS_0628(hylB)

SDC:	SDSE_0694(hylB)

SDQ:	SDSE167_0713(hylB)

STK:

STP_1167

SIE:

SCIM_1362

SIB:

SIR_1547

SIU:

SII_1533

SANC:

SANR_1718

SCG:

SCI_1642

SCON:

SCRE_1598

SCOS:

SCR2_1598

SIK:

K710_0217

SIQ:	DQ08_01045

SIO:	DW64_01040

SIZ:	SI82_01295

SRQ:	SR187_3375

SMEN:

SAMEA4412692_1386(hylA)

SURN:

NCTC13766_00112(hylB_1)

SOS:	INT76_10670

SZG:	J5M87_09090

EFA:

EF0818

EFI:	OG1RF_10550

EFD:	EFD32_0629

ENE:

ENT_22740

EFT:	M395_11255

EMU:

EMQU_2462

EGA:	AL523_06180

ESS:	ATZ33_14390

ETH:	CK496_10330

ELAC:

I4Q40_11295

ECEC:

NCTC12421_00019

EIE:	ENLAB_20640

ENI:	CXM95_01160

ERX:	ATZ35_06015

TLJ:	I4Q36_06855

GAD:	K8O88_08775

GEG:	LK443_01000

BHAN:

CGC63_14875

HOV:	EQF90_007695

HKU:	HIF96_07580

ERH:	ERH_0765(hylB)

ERS:	K210_01660

BULL:

JOS54_07575

SCO:	SCO5534(SC1C2.15)

SMA:	SAVERM_2704

SGR:

SGR_291

SGB:

WQO_33115

SFI:

SFUL_16

SANL:

KZO11_37140

SGRF:

SGFS_040030

SCYE:

R2B67_00610

SCB:	SCAB_26801

SHY:

SHJG_6645

SHO:	SHJGH_6405

SVE:

SVEN_5221

SDV:	BN159_0958 BN159_2862

SALS:

SLNWT_4661

SCI:	B446_25985

SLV:	SLIV_10835

SGU:	SGLAU_23805

SVT:	SVTN_26825

STRE:

GZL_03204

SCW:	TU94_23365

SLD:

T261_2519

SLC:	SL103_28195

STRC:

AA958_24125

SAMB:

SAM23877_5289

SPRI:

SPRI_4720

SCZ:	ABE83_00480

SCX:	AS200_15735

SRW:	TUE45_06045(xly)

SLE:	sle_21210(sle_21210)

SPAV:

Spa2297_23435

STRT:

A8713_00365 A8713_22525

SCLF:

BB341_06495

SGS:	AVL59_08750

STSI:

A4E84_28340

SALW:

CP975_31340

SGM:	GCM10017557_23350

SLS:	SLINC_5976

SNR:	SNOUR_13955

SALU:

DC74_5664

SALL:

SAZ_29920

STRD:

NI25_11545

SVU:	B1H20_33735

SPUN:

BFF78_03435

SGV:	B1H19_28315

SLAU:

SLA_5431

SALF:

SMD44_01144

SALJ:

SMD11_2031

SLX:	SLAV_04325(xly)

STRO:

STRMOE7_27170

SFK:

KY5_5711

SNZ:	DC008_24835

SGE:	DWG14_02529(xly)

SRJ:

SRO_0644

SVQ:	N6Q81_24720

SLK:	SLUN_28075

SGD:	ELQ87_11925

SQZ:	FQU76_24730

SCYA:

EJ357_32680

SAST:

CD934_09335

SKA:	CP970_04240 CP970_12530

SVN:	CP980_03870

SHAW:

CEB94_28975

SRK:	FGW37_23350

SFIC:

EIZ62_08725

SPAD:

DVK44_25640

SAQU:

EJC51_41950

SGF:	HEP81_00629

SCAV:

CVT27_32625

SRIM:

CP984_11465

SSUB:

CP968_31185

SPHV:

F9278_35050

SCIN:

CP977_33110

SPAC:

B1H29_10935

STSU:

B7R87_24740

SCHA:

CP983_12950

SGX:	H4W23_28340

SCOE:

CP976_30575

SSPB:

CP982_06315

SPLA:

CP981_27530

SBRO:

GQF42_39675

STUI:

GCM10017668_50460

SATA:

C5746_03170 C5746_29510 C5746_36050

SHUN:

DWB77_02354(xly_1) DWB77_06443(xly_2) DWB77_06490(xly_3)

SCYG:

S1361_27505(xly)

SFEU:

IM697_35535

SLIA:

HA039_09355

SDW:	K7C20_26190

SCAL:

I6J39_33470

SAUH:

SU9_024925

SMOB:

J7W19_23170

SXN:	IAG42_02145 IAG42_05180 IAG42_32435

SGJ:	IAG43_30120

SHK:	J2N69_32955

SANU:

K7396_10630

SDD:	D9753_10340

SACT:

DMT42_10045

SGOB:

test1122_22970

SINE:

KI385_31415

SLF:	JEQ17_14750

SDEC:

L3078_32175

SAOV:

G3H79_33140

SDUR:

M4V62_03810 M4V62_07500

SAKB:

K1J60_13055

STAA:

LDH80_12570

SNIG:

HEK616_54990

SCAE:

IHE65_12565

STUB:

MMF93_02035

SSPN:

LXH13_28065

SCIR:

STRCI_005716 STRCI_007476

SXT:	KPP03845_106619(xly_2)

SLON:

LGI35_30395 LGI35_35640

SDRZ:

NEH16_03190

STUD:

STRTU_002005

SENG:

OJ254_01650

SYUN:

MOV08_13800

SARG:

HKX69_09970

SJN:	RI060_11845

SOV:	QZH56_08835

SHAU:

K9S39_14835

SKG:	KJK29_09930

SCOA:

QU709_11630

SLAI:

P8A22_02080 P8A22_03890 P8A22_36685

SROC:

RGF97_30970

SFP:	QUY26_35460

SCHG:

NRO40_21400

SINN:

ABB07_02650

KBU:	Q4V64_37430

STPB:

QR97_09255

SRPA:

HEP86_28570

SMIB:

SMIR_37530

SCAJ:

O1G22_12885

STRW:

QHG49_32270

STRL:

HEP84_07625

SCHE:

QRN89_24785

SCAT:

P8A18_30695

STII:

DRB96_38510

STIC:

DRB89_38035

SABD:

N8I86_26775

STRV:

EZV63_25850

SMOD:

POD33_21060

SPOF:

P8A21_01270 P8A21_28625 P8A21_35515

STBR:

ABDE16_10260

STAC:

ABII15_37180

STVB:

OH133_00845 OH133_37050

SSOC:

ABZO31_23995

SSLI:

R2D22_00795 R2D22_10985

STYI:

C9F11_05885(xly1)

SCIT:

V2W30_02855 V2W30_34375 V2W30_37385

SSIR:

WAB15_28820

SEXP:

SLV14_002298

SSTO:

ACE1OC_02730

SLUA:

AB8735_26620

STGO:

KBP30_13220

STCM:

SCMC78_01300 SCMC78_72220

STID:

G7Z12_10535

STCZ:

JHY03_67320

STHU:

M1P56_26885

STJL:

ACE1OA_10130

STHP:

MQE23_13540

STRX:

A6E92_00270

STCL:

CAC01_03330

STFI:

WID27_00255

STPG:

C1703_28055(xly)

STPJ:

R1Y80_34805

SCT:

SCAT_4363

SCY:	SCATT_43510

KAB:	B7C62_00095

KIC:	KCMC57_up07130

PPOD:

AB0761_32440

STRH:

GXP74_30850

MEST:

PTQ19_00485

ARR:	ARUE_c32170

ARE:	AL755_00180

ARN:	CGK93_17430

AWW:	G8758_10730

AAU:

AAur_3068

PNV:	JMY29_14850

PAGO:

IRJ34_15385

PAYN:

HMI60_14945

BCV:

Bcav_4062

LMOI:

VV02_06880

HALT:

IM660_00335 IM660_14490

RHAL:

LQF10_05115 LQF10_16250

PAC:

PPA0380

PAK:	HMPREF0675_3415

PAV:	TIA2EST22_01875

PAX:	TIA2EST36_01855

PAZ:	TIA2EST2_01795

PAW:	PAZ_c03930(hylB)

PAD:	TIIST44_06625

PCN:	TIB1ST10_01945

PACC:

PAC1_01925

PACH:

PAGK_0394

PACN:

TIA1EST1_01875

CACN:

RN83_02370

PRL:	BCB70_04600

MIK:	FOE78_16715

CZN:	ACQBAR_01915

KFL:

Kfla_2021

KQI:	F1D05_13565

KSL:	OG809_24590

KRI:	OX958_07760

SRO:

Sros_4208

NOA:	BKM31_31155 BKM31_46745 BKM31_51920

NOW:	GBF35_40595 GBF35_50865

NCX:	Nocox_41810(xly4)

NGN:	LCN96_14075 LCN96_49450 LCN96_55380

NFS:	OIE67_46880 OIE67_54920

MHAI:

OHB01_09145

KUT:	JJ691_100600 JJ691_33230

KBR:	Q4V65_36940 Q4V65_46455

AAUR:

V5P93_003318

CROS:

N8J89_17545

ACTE:

ACTI_51170

ATL:	Athai_07180 Athai_37990 Athai_61400 Athai_66290

CAI:

Caci_6725

GLY:	K3N28_09530

FMO:	C4N19_04620

CSOM:

MKD34_01125

SMF:

Smon_0117

HMU:

Hmuk_3227

HALZ:

E5139_16020

» show all

Reference

1 [PMID:13762462]

Authors

LINKER A, HOFFMAN P, MEYER K, SAMPSON P, KORN ED.

Title

The formation of unsaturated disacharides from mucopoly-saccharides and their cleavage to alpha-keto acid by bacterial enzymes.

Journal

J Biol Chem 235:3061-5 (1960)

Reference

2 [PMID:14943668]

Authors

MEYER K, RAPPORT MM.

Title

Hyaluronidases.

Journal

Adv Enzymol Relat Subj Biochem 13:199-236 (1952)
DOI:10.1002/9780470122587.ch6

Reference

3 [PMID:5642600]

Authors

Moran F, Nasuno S, Starr MP.

Title

Extracellular and intracellular polygllacturonic acid trans-eliminases of Erwinia carotovora.

Journal

Arch Biochem Biophys 123:298-306 (1968)
DOI:10.1016/0003-9861(68)90138-0

Other DBs

ExplorEnz - The Enzyme Database:

4.2.2.1

IUBMB Enzyme Nomenclature:

4.2.2.1

ExPASy - ENZYME nomenclature database:

4.2.2.1

BRENDA, the Enzyme Database:

4.2.2.1

CAS:	37259-53-3

LinkDB

DBGET integrated database retrieval system