Entry
Name
canavanine-gamma-lyase;
CangL
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
L-canavanine N-hydroxyguanidine-lyase (L-homoserine-forming)
Reaction(IUBMB)
L-canavanine + H2O = L-homoserine + N-hydroxyguanidine (overall reaction) [RN:
R13115 ];
(1a) L-canavanine = vinylglycine + N-hydroxyguanidine [RN:
R13112 ];
(1b) vinylglycine = (2E)-2-aminobut-2-enoate (spontaneous) [RN:
R13113 ];
(1c) (2E)-2-aminobut-2-enoate + H2O = L-homoserine (spontaneous) [RN:
R13114 ]
Reaction(KEGG)
Substrate
Product
L-homoserine [CPD:
C00263 ];
N-hydroxyguanidine [CPD:
C22626 ];
vinylglycine [CPD:
C22624 ];
(2E)-2-aminobut-2-enoate [CPD:
C22625 ]
Comment
A pyridoxal 5'-phosphate protein. The enzyme, characterized from the bacterium Pseudomonas canavaninivorans, cleaves a carbon-oxygen bond, releasing N-hydroxyguanidine and an unstable enamine product that tautomerizes to an imine form, which is attacked by a water molecule to form L-homoserine.
History
EC 4.4.1.43 created 2022
Orthology
Genes
VSR : Vspart_01493(mccB_1)
MHUA : MCHK_11665 MCHK_7242
SFD : USDA257_c26390(mdeA2)
SAME : SAMCFNEI73_pC1887(mdeA)
EAK : EKH55_4889 EKH55_5636 EKH55_5829
MANA : MAMMFC1_00245(metB)
SACG : FDZ84_25750 FDZ84_35495
» show all
Taxonomy
Reference
Authors
Hauth F, Buck H, Stanoppi M, Hartig JS.
Title
Canavanine utilization via homoserine and hydroxyguanidine by a PLP-dependent gamma-lyase in Pseudomonadaceae and Rhizobiales.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.43
ExPASy - ENZYME nomenclature database: 4.4.1.43
LinkDB
All DBs