Entry
Name
GDP-mannose 3,5-epimerase;
GME (gene name);
GDP-D-mannose:GDP-L-galactose epimerase;
guanosine 5'-diphosphate D-mannose:guanosine 5'-diphosphate L-galactose epimerase
Class
Isomerases;
Racemases and epimerases;
Acting on carbohydrates and derivatives
BRITE hierarchy
Sysname
GDP-alpha-D-mannose 3,5-epimerase
Reaction(IUBMB)
(1) GDP-alpha-D-mannose = GDP-beta-L-galactose [RN:
R00889 ];
(2) GDP-alpha-D-mannose = GDP-beta-L-gulose [RN:
R07672 ]
Reaction(KEGG)
Substrate
GDP-alpha-D-mannose [CPD:
C00096 ]
Product
GDP-beta-L-galactose [CPD:
C02280 ];
GDP-beta-L-gulose
Comment
The enzyme catalyses the formation of the stable intermediate GDP-beta-L-gulose as well as GDP-beta-L-galactose. The reaction proceeds by C4' oxidation of GDP-alpha-D-mannose followed by epimerization of the C5' position to give GDP-beta-L-4-dehydro-gulose. This intermediate is either reduced to give GDP-beta-L-gulose or the C3' position is epimerized to give GDP-beta-L-4-dehydro-galactose, followed by C4' reduction to yield GDP-beta-L-galactose. Both products serve as intermediates in two different variants of plant L-ascorbate biosynthesis pathways.
History
EC 5.1.3.18 created 1986, modified 2020
Pathway
ec00053 Ascorbate and aldarate metabolism
ec00520 Amino sugar and nucleotide sugar metabolism
ec01110 Biosynthesis of secondary metabolites
Orthology
K10046 GDP-D-mannose 3', 5'-epimerase
Genes
CSAT : 104707129 104736843 104771306
BRP : 103854620 103874594(GME)
BNA : 106348783 106348855 106367760 106382501
RSZ : 108809026 108836738 108855455
CIC : CICLE_v10001524mg CICLE_v10006605mg
MINC : 123209084 123221942
GHI : 107894853 107899156 107936906 121212442
HSYR : 120113854 120150411 120154233 120183126
EGR : 104415433 104423523 104432512
GMX : 100527798(GME1) 100780764 100794772 100797618 100806498
GSJ : 114371997 114398160 114400533 114403164 114407699
PVU : PHAVU_006G101600g PHAVU_007G187300g
VUM : 124819469 124845500 124845501
CCAJ : 109791853 109797468
APRC : 113861202 113871018
TPRA : 123889674 123903116
PSAT : 127091812 127132236
VVO : 131610662 131644143 131644144
LJA : Lj0g3v0283729.1(Lj0g3v0283729.1) Lj0g3v0283729.2(Lj0g3v0283729.2) Lj0g3v0283729.3(Lj0g3v0283729.3) Lj0g3v0283729.4(Lj0g3v0283729.4) Lj0g3v0283729.5(Lj0g3v0283729.5) Lj0g3v0283729.6(Lj0g3v0283729.6) Lj1g3v4979970.1(Lj1g3v4979970.1)
LANG : 109336358 109354284
PCIN : 129284651 129307171
QSA : O6P43_007627 O6P43_011209
PMUM : 103318635 103324176
PAVI : 110754286 110760949
PDUL : 117613821 117630761
MDM : 103408656 103430505 103434162
MSYL : 126593036 126618813 126618814
MNT : 112092678 21385667 21396531
CSAV : 115719031 115724084
CMAX : 111465572 111493789
CMOS : 111444302 111450536
CPEP : 111784760 111798673 111805991
HBR : 110648686 110662568 110671312
MESC : 110621205 110623092
PALZ : 118044041 118051352
CILL : 122274241 122274536 122277832 122282505 122316821
CAVE : 132171353 132171363
SLY : 100316874(GME2) 100316902(GME1)
SPEN : 107006921 107031214
SSTN : 125869350 125870680
SDUL : 129883066 129886329
CANN : 107839422 107864663
NTA : 107797479(GME1) 107812799 107817046 107826294
SIND : 105158417 105171617
OEU : 111376426 111383576 111393710
SSPL : 121773719 121777196 121777197 121806859
SHIS : 125197419 125200081 125200143 125202055
APAN : 127250639 127266620
CSIN : 114298671 114323341
ATRI : 130796845 130817781
NNU : 104602849 104609581 104613267
MING : 122090027 122092035
PSOM : 113318033 113350016 113360896
DOSA : Os10t0417600-01(Os10g0417600) Os11t0591100-01(Os11g0591100)
TDC : 119292229 119302474 119311151 119349586
TAES : 123068544 123104418 123112716 123122194 123146299 123183154
LPER : 127295215 127319608
LRD : 124652610 124655088 124702932 124702941
SITA : 101752979 101772247 101778042
SVS : 117833479 117839953 117860607
PVIR : 120644778 120651043 120678581 120685784 120690158 120711419
PHAI : 112876237 112897224 112902534
PDA : 103695731 103716162 103718012
EGU : 105046574 105053413 105055709
MUS : 103975282 103978838 103990359 103993866
ZOF : 121972179 121977197 121982095 121985972 122051284 122055803
MSIN : 131232682 131248929
NCOL : 116256868 116258167
SMO : SELMODRAFT_110416 SELMODRAFT_128181 SELMODRAFT_157784 SELMODRAFT_178232 SELMODRAFT_272082 SELMODRAFT_406366 SELMODRAFT_76013 SELMODRAFT_76257 SELMODRAFT_79000 SELMODRAFT_83391
PPP : 112276157 112281831 112294838
MIS : MICPUN_80077 MICPUN_92683
MPP : MICPUCDRAFT_46037(JGI:MicpuC2_46037)
FCY : FRACYDRAFT_268332(RMLB2_UXS2_1)
TPS : THAPSDRAFT_41548(GALE3)
TVA : TVAG_2v0240090 TVAG_2v0728360
TSV : DSM104635_00202(gerKI)
ABAS : ACPOL_1917 ACPOL_5967
SRM : SRM_00247(wcaG) SRM_00716(wcaG)
» show all
Taxonomy
Reference
Authors
Hebda PA, Behrman EJ, Barber GA.
Title
The guanosine 5'-diphosphate D-mannose: guanosine 5'-diphosphate L-galactose epimerase of Chlorella pyrenoidosa. Chemical synthesis of guanosine 5'-diphosphate L-galactose and further studies of the enzyme and the reaction it catalyzes.
Journal
Reference
Authors
Barber GA, Hebda PA.
Title
GDP-D-mannose: GDP-L-galactose epimerase from Chlorella pyrenoidosa.
Journal
Reference
Authors
Wolucka BA, Persiau G, Van Doorsselaere J, Davey MW, Demol H, Vandekerckhove J, Van Montagu M, Zabeau M, Boerjan W
Title
Partial purification and identification of GDP-mannose 3",5"-epimerase of Arabidopsis thaliana, a key enzyme of the plant vitamin C pathway.
Journal
Sequence
Reference
Authors
Major LL, Wolucka BA, Naismith JH
Title
Structure and function of GDP-mannose-3',5'-epimerase: an enzyme which performs three chemical reactions at the same active site.
Journal
Sequence
Reference
Authors
Watanabe K, Suzuki K, Kitamura S
Title
Characterization of a GDP-D-mannose 3'',5''-epimerase from rice.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 5.1.3.18
ExPASy - ENZYME nomenclature database: 5.1.3.18
LinkDB
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