KEGG ENZYME: 6.2.1.43

ENZYME: 6.2.1.43

Entry

EC 6.2.1.43                 Enzyme

Name

2-hydroxy-7-methoxy-5-methyl-1-naphthoate---CoA ligase;
NcsB2

Class

Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases

Sysname

2-hydroxy-7-methoxy-5-methyl-1-naphthoate:CoA ligase

Reaction(IUBMB)

ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA = AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA [RN:R10771]

Reaction(KEGG)

R10771

Substrate

ATP [CPD:C00002];
2-hydroxy-7-methoxy-5-methyl-1-naphthoate [CPD:C20841];
CoA [CPD:C00010]

Product

AMP [CPD:C00020];
diphosphate [CPD:C00013];
2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA [CPD:C20842]

Comment

The enzyme from the bacterium Streptomyces carzinostaticus is involved in the attachment of the 2-hydroxy-7-methoxy-5-methyl-1-naphthoate moiety of the antibiotic neocarzinostatin. In vitro the enzyme also catalyses the activation of other 1-naphthoic acid analogues, e.g. 2-hydroxy-5-methyl-1-naphthoate or 2,7-dihydroxy-5-methyl-1-naphthoate.

History

EC 6.2.1.43 created 2014

Pathway

ec01059

Biosynthesis of enediyne antibiotics

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K20423

2-hydroxy-7-methoxy-5-methyl-1-naphthoate---CoA ligase

Genes

PROD:

PCO85_04745

BSAV:

WS86_20930

BHG:	I6G56_26270

CBRE:

QP027_12190

MTS:

MTES_3604

ARTP:

E5206_14865

APN:	Asphe3_39850

PSUL:

AU252_00410

AOI:	AORI_6598(dhbE)

AORI:

SD37_05095

AROO:

NQK81_30345

SAQ:

Sare_4933

MICH:

FJK98_15490

ACTN:

L083_3190

» show all

Reference

1 [PMID:17539640]

Authors

Cooke HA, Zhang J, Griffin MA, Nonaka K, Van Lanen SG, Shen B, Bruner SD

Title

Characterization of NcsB2 as a promiscuous naphthoic acid/coenzyme A ligase integral to the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin.

Journal

J Am Chem Soc 129:7728-9 (2007)
DOI:10.1021/ja071886a

Sequence

[ag:AAM77987]

Other DBs

ExplorEnz - The Enzyme Database:

6.2.1.43

IUBMB Enzyme Nomenclature:

6.2.1.43

ExPASy - ENZYME nomenclature database:

6.2.1.43

BRENDA, the Enzyme Database:

6.2.1.43

LinkDB

DBGET integrated database retrieval system