KEGG ENZYME: 6.3.4.22

ENZYME: 6.3.4.22

Entry

EC 6.3.4.22                 Enzyme

Name

tRNAIle2-agmatinylcytidine synthase;
TiaS;
AF2259;
tRNAIle-2-agmatinylcytidine synthetase;
tRNAIle-agm2C synthetase;
tRNAIle-agmatidine synthetase

Class

Ligases;
Forming carbon-nitrogen bonds;
Other carbon-nitrogen ligases

Sysname

agmatine:[tRNAIle]-cytidine34 ligase

Reaction(IUBMB)

ATP + agmatine + [tRNAIle2]-cytidine34 + H2O = [tRNAIle2]-2-agmatinylcytidine34 + AMP + 2 phosphate [RN:R10496]

Reaction(KEGG)

R10496

Substrate

ATP [CPD:C00002];
agmatine [CPD:C00179];
[tRNAIle2]-cytidine34;
H2O [CPD:C00001]

Product

[tRNAIle2]-2-agmatinylcytidine34;
AMP [CPD:C00020];
phosphate [CPD:C00009]

Comment

The enzyme from the archaeon Archaeoglobus fulgidus modifies the wobble base of the CAU anticodon of the archaeal tRNAIle2 at the oxo group in position 2 of cytidine34. This modification is crucial for accurate decoding of the genetic code. In bacteria EC 6.3.4.19, tRNAIle-lysidine synthase, catalyses the modification of [tRNAIle2]-cytidine34 to [tRNAIle2]-lysidine34 .

History

EC 6.3.4.22 created 2013

Orthology

K06932

tRNA(Ile2)-agmatinylcytidine synthase

Genes

MJA:

MJ_1095

MFE:	Mefer_1336

MVU:	Metvu_1118

MFS:	MFS40622_0162

MIF:	Metin_0603

MJH:	JH146_0033

MESG:

MLAUSG7_0809(tiaS)

MESA:

MLASG1_0384(tiaS)

MIG:	Metig_0478

MMP:

MMP0616

MMQ:	MmarC5_0989

MMX:	MmarC6_0275

MMZ:	MmarC7_1639

MMD:

GYY_03390

MMAK:

MMKA1_06660

MMAO:

MMOS7_06610

MMAD:

MMJJ_03670

MAE:

Maeo_0755

MVN:	Mevan_1489

MVO:

Mvol_0248

MOK:	Metok_0217

METF:

CFE53_03130

MTH:

MTH_966

MMG:	MTBMA_c13520

MWO:	MWSIV6_1356(tiaS)

MTEE:

MTTB_04510

METC:

MTCT_0877

METE:

tca_00929

METZ:

METMT2_0998

METK:

FVF72_06765

MTHM:

FZP57_06470

METJ:

FZP68_04195

MST:

Msp_1142

METB:

AW729_10065

MEIS:

PXD04_01950

MRU:

mru_0685

MSI:

Msm_0452

MEB:

Abm4_0413

MMIL:

sm9_0558

MEYE:

TL18_02620

MOL:

YLM1_0329

MARO:

MarbSA_17680

METV:

K4897_02780

MEL:	Metbo_0426

MEW:	MSWAN_1961

METH:

MBMB1_1591(tiaS)

MFC:

BRM9_0826

MFI:	DSM1535_0789(tiaS)

MCUB:

MCBB_1889(tiaS)

MSUB:

BK009_06950

METN:

BK008_02590

METT:

CIT01_02575

METO:

CIT02_09995

MEME:

HYG87_03930

MFEG:

GCM10025860_01320

MFV:

Mfer_0668

MKA:

MK1003

AFU:

AF_2259

AFG:	AFULGI_00025540

APO:	Arcpr_0648

AVE:	Arcve_1578

AST:	Asulf_01007

FPL:

Ferp_1288

GAC:

GACE_1471

GAH:

GAH_01052

PFU:

PF1855

PFI:

PFC_09315

PHO:	PH1812(PH1812)

PAB:

PAB0247

PYN:

PNA2_0411

PYA:	PYCH_01040

PYS:

Py04_1738

PYC:	TQ32_07680

TKO:

TK0553

TON:

TON_0699

TGA:

TGAM_1029

TSI:

TSIB_1197

TBA:	TERMP_00575

THE:

GQS_01425

THA:

TAM4_479

THM:

CL1_2002

TLT:

OCC_01114

THS:

TES1_0950

TNU:

BD01_0657

TEU:

TEU_08925

TGY:	X802_09150

THV:	ADU37_CDS09310

TCH:	CHITON_2099

TPEP:

A0127_01940

TPIE:

A7C91_09615

TGG:	A3K92_03195

TCE:	A3L02_03845

TBS:	A3L01_04265

THH:	CDI07_04160

TSL:	A3L11_08280

TTD:	A3L14_06160

TPRF:

A3L09_07025

TRL:	A3L10_02330

TPAF:

A3L08_07615

THY:	A3L12_07690

TIC:	FH039_07920

TCQ:	TIRI35C_1105(tiaS)

THEM:

FPV09_01495

THEI:

K1720_09035

PPAC:

PAP_07755

MBA:	Mbar_A2319

MBY:	MSBRM_1009

MBW:	MSBRW_2425

MBAR:

MSBR2_1401

MBAK:

MSBR3_1473

MAC:

MA_3525

MMA:

MM_0445

MMJ:	MSMAS_0858

MMAC:

MSMAC_0847

MVC:	MSVAZ_1055

MEK:	MSKOL_1005

MLS:	MSLAZ_0746

METM:

MSMTP_2364

MEF:	MSWH1_0641

MEQ:	MSWHS_0661

MSJ:	MSSAC_3216

MSZ:	MSSIH_1046

MSW:	MSSIT_1093

MTHR:

MSTHT_1079

MTHE:

MSTHC_2224

MHOR:

MSHOH_3068

MFZ:	AOB57_011030

MBU:

Mbur_1810

MMET:

MCMEM_0061

MELO:

J7W08_10385

MMH:

Mmah_1918

MHAZ:

BHR79_04050

MPOT:

BKM01_00095

MEV:	Metev_0063

MZH:	Mzhil_0058

MPY:

Mpsy_1823

MZI:	HWN40_12975

MMAV:

RE476_10575

MSEB:

RE474_07185

MHZ:	Metho_2324

MEHF:

MmiHf6_17810

MEES:

MmiEs2_09740

MTP:

Mthe_0611

MCJ:

MCON_1904

MHI:

Mhar_0187

MHU:

Mhun_2547

MRTJ:

KHC33_03945

MLA:

Mlab_0159

MEM:	Memar_2348

MBG:	BN140_2487

MEMA:

MMAB1_3347(tiaS)

MCHK:

MchiMG62_00780

MSUM:

OH143_06010

MRC:	R6Y96_01490

MPI:

Mpet_0081

MEND:

L6E24_07650

MEFW:

F1737_10600

MAQE:

RJ40_08600

MFK:	E2N92_08810

MOU:	OU421_07960

MESB:

L1S32_11315

MANQ:

L1994_10580

MBN:

Mboo_0194

MFO:	Metfor_2916

MPL:

Mpal_0030

MPD:

MCP_2871

MEZ:

Mtc_0281

RCI:

RCIX1429

HAL:

VNG_2110C

HSL:	OE_3943R(tiaS)

HANR:

LJ422_08480

HDL:	HALDL1_15450

HHB:	Hhub_3400(tiaS)

HAGS:

JT689_09830

HABO:

JRZ79_08210

HNO:	LT974_13820

HLU:	LT972_00925

HALH:

HTSR_1874

HHSR:

HSR6_1944(tiaS)

HSU:	HLASF_0230

HSF:	HLASA_0230

HAHS:

HSRCO_0365(tiaS)

SALR:

FQU85_05615

HALR:

EFA46_002530

HJE:	HacjB3_01490

HDO:	MUK72_04960

HALX:

M0R89_06930

HAXZ:

M0R88_07150

HAYC:

NGM10_09045

HAXY:

NGM07_12360

SALI:

L593_15020

SAIM:

K0C01_04760

HARA:

AArcS_2537(tiaS)

HMA:

rrnAC2518

HHI:

HAH_2948

HHN:	HISP_14990

HAB:	SG26_07105

HTA:	BVU17_14330

HALJ:

G9465_03480

HSIN:

KDQ40_14520

NPH:	NP_1318A(tiaS)

NMO:	Nmlp_3253(tiaS)

NHO:	HWV23_06135

NSAL:

HWV07_07860

HUT:

Huta_1790

HTI:

HTIA_1320

HALA:

Hrd1104_11295

HASV:

SVXHr_2760(tiaS)

HABN:

HBNXHr_2699(tiaS)

HMU:

Hmuk_0845

HALZ:

E5139_07405

HALL:

LC1Hm_0272(tiaS)

HAZP:

GBQ70_07400

HALI:

BV210_08930

HSN:	DV733_09715

HDS:	HSR122_2452(tiaS)

HRR:	HZS55_03390

HPEL:

HZS54_04090

HLT:	I7X12_10240

HARC:

HARCEL1_13145

SSAI:

N0B31_18155

HAAD:

MW046_08200

HWA:	HQ_3056A(tiaS)

HWC:	Hqrw_3574(tiaS)

HVO:	HVO_0339(tiaS)

HME:

HFX_0324

HGI:	ABY42_01535

HALE:

G3A49_01910

HLS:	KU306_06385

HLN:	SVXHx_0320(tiaS)

HBO:	Hbor_28320

HAQ:	DU484_04065

HAJ:	DU500_04545

HAER:

DU502_02260

HRA:	EI982_11255

HLM:	DV707_02645

HALM:

FCF25_08435

HLA:

Hlac_0272

HALP:

DOS48_01445

HALB:

EKH57_10265

HEZZ:

EO776_01165

HALQ:

Hrr1229_005530

HSS:	J7656_00015

HSAI:

HPS36_05490

HAZZ:

KI388_14840

SRUB:

C2R22_01410

HAE:	halTADL_2102

HALN:

B4589_010000

HALG:

HUG10_01770

HALU:

HUG12_15320

HMP:	K6T50_15165

HRE:	K6T36_15165

HRM:	K6T25_01180

HACB:

Hbl1158_07255

HDF:	AArcSl_0862(tiaS)

HAH:	Halar_3068

HTU:

Htur_2426

HSAL:

JMJ58_16720

HAKZ:

J0X25_00650

NMG:	Nmag_1178(tiaS)

HXA:	Halxa_4156

NAT:

NJ7G_1333

NPE:	Natpe_1372

NVR:	FEJ81_07255

NPL:	FGF80_04085

NAY:	HYG81_03335

HALY:

HYG82_05250

HJT:	DVR14_16035 DVR14_20155

HLO:	J0X27_17760

NGE:	Natgr_2460

HRU:	Halru_2032

SAWL:

NGM29_07480

HALV:

NGM15_16670

NOU:	Natoc_1343

HLR:	HALLA_08665

HLC:	CHINAEXTREME10490

NAJ:	B1756_16205

NAG:	AArcMg_2515

NAN:	AArc1_1212

NARA:

QQ977_14185

NBG:	DV706_11465

NAS:	GCU68_00660

HDA:	BB347_08245

MELU:

MTLP_08490

MEAM:

MU439_00920

MEAE:

QEN48_01685

TAC:

Ta0545

TVO:	TVG0590669(TVG0590669)

PTO:

PTO1377

FAC:	FACI_IFERC01G1593

FAI:	FAD_0572(tiaS)

CDIV:

CPM_0360

TAR:	TALC_01575

MAX:	MMALV_16950

MER:	MMINT_19450

MEAR:

Mpt1_c14580(tiaS2)

MEUZ:

KRP56_00315

MARC:

AR505_0003

ABI:

Aboo_0928

ACF:	AciM339_0076

APE:	APE_0656.1

ACJ:

ACAM_0488

SMR:

Smar_1362

SHC:	Shell_1082

IHO:

Igni_0309

IIS:

EYM_07450

DKA:

DKAM_0469

DFD:	Desfe_0589

DMU:	Desmu_0728

TAG:

Tagg_0667

IAG:

Igag_0055

THG:	TCELL_0822

HBU:

Hbut_0008

PFM:	Pyrfu_0159

PDL:	Pyrde_0549

PABI:

PABY_03050(tiaS)

PARE:

PYJP_15050(tiaS)

STO:

STK_03120

SOH:	D1869_01120

SSO:

SSO0258

SOL:

Ssol_1234

SSOA:

SULA_1275

SSOL:

SULB_1276

SSOF:

SULC_1274

SSHI:

J5U23_03210

SCAS:

SACC_01760

SAI:

Saci_0732

SACN:

SacN8_03555

SACR:

SacRon12I_03540

SACS:

SUSAZ_03310

SIS:	LS215_1991

SIA:	M1425_1882

SIM:	M1627_1959

SID:

M164_1889

SIY:	YG5714_1958

SIN:	YN1551_0962

SII:

LD85_2103

SIH:

SiH_1819

SIR:

SiRe_1739

SIC:

SiL_1732

SULA:

BFU36_04715

SULE:

GFS03_10310

SULO:

GFS33_02125

SULL:

EWF20_02585

MSE:

Msed_2296

MEMJ:

MJ1HA_2625

MCN:

Mcup_2039

MHK:	DFR87_06960

MPRU:

DFR88_06700

MTEN:

GWK48_05200

MJN:	MjAS7_0586

AHO:

Ahos_0781

AMAN:

B6F84_07360

ABRI:

DFR85_07565

ASUL:

DFR86_06420

AAMB:

D1866_11735

ACIH:

HS5_21550

SACD:

HS1genome_0236

SAZO:

D1868_01910

CSTY:

KN1_18760

STEP:

IC006_1793

SMET:

RQ359_000050

SAHS:

HS7_07740

PAI:

PAE0933

PIS:

Pisl_1170

PCL:

Pcal_0100

PAS:

Pars_0133

PYR:

P186_2378

POG:

Pogu_2352

TNE:

Tneu_0126

PYW:	PYWP30_00136

CMA:

Cmaq_0160

TTN:

TTX_0548

TUZ:

TUZN_0192

VDI:

Vdis_1829

VMO:

VMUT_0270

VSO:	Vsou_02260

TPE:

Tpen_0604

THB:	N186_04390

TCB:	TCARB_1905

THEL:

IG193_04485

ASC:

ASAC_1057

ACIA:

SE86_06420

CLG:	Calag_0571

FFO:	FFONT_0807

NMR:

Nmar_1562

NIR:	NSED_00695

NKR:	NKOR_08500

NID:	NPIRD3C_0493(tiaS)

NIW:	Nisw_06090

NCL:	C5F47_08190

NOX:	C5F49_08075

NUE:	C5F50_11435

NIP:	NsoK4_08340

NZT:	NZOSNM25_000225

NCT:	NMSP_0197(tiaS)

NIC:	DSQ20_09100

NGA:	Ngar_c35910

NVN:	NVIE_020600

NEV:

NTE_00071

TAA:	NMY3_00488

NFN:	NFRAN_1460(tiaS)

NCV:	NCAV_0167(tiaS)

CSY:	CENSYa_2023

CSU:	CSUB_C1139

NBV:

T478_1284

TAH:	SU86_004625

NIU:	DSQ19_01395

NDV:	NDEV_1925(tiaS)

CCAI:

NAS2_0020

BARC:

AOA65_1257(tiaS_1) AOA65_1587(tiaS_2)

BARB:

AOA66_1422(tiaS)

» show all

Reference

1 [PMID:20139989]

Authors

Ikeuchi Y, Kimura S, Numata T, Nakamura D, Yokogawa T, Ogata T, Wada T, Suzuki T, Suzuki T

Title

Agmatine-conjugated cytidine in a tRNA anticodon is essential for AUA decoding in archaea.

Journal

Nat Chem Biol 6:277-82 (2010)
DOI:10.1038/nchembio.323

Sequence

[afu:AF_2259] [pho:PH1812]

Reference

2 [PMID:22002222]

Authors

Terasaka N, Kimura S, Osawa T, Numata T, Suzuki T

Title

Biogenesis of 2-agmatinylcytidine catalyzed by the dual protein and RNA kinase TiaS.

Journal

Nat Struct Mol Biol 18:1268-74 (2011)
DOI:10.1038/nsmb.2121

Reference

3 [PMID:22102245]

Authors

Osawa T, Inanaga H, Kimura S, Terasaka N, Suzuki T, Numata T

Title

Crystallization and preliminary X-ray diffraction analysis of an archaeal tRNA-modification enzyme, TiaS, complexed with tRNA(Ile2) and ATP.

Journal

Acta Crystallogr Sect F Struct Biol Cryst Commun 67:1414-6 (2011)
DOI:10.1107/S1744309111034890

Other DBs

ExplorEnz - The Enzyme Database:

6.3.4.22

IUBMB Enzyme Nomenclature:

6.3.4.22

ExPASy - ENZYME nomenclature database:

6.3.4.22

BRENDA, the Enzyme Database:

6.3.4.22

LinkDB

DBGET integrated database retrieval system