KEGG   REACTION: R05463
Entry
R05463                      Reaction                               
Name
6-methylpretetramide,NADPH:oxygen oxidoreductase (4-hydroxylating)
Definition
6-Methylpretetramide + NADPH + Oxygen + H+ <=> 4-Hydroxy-6-methylpretetramide + NADP+ + H2O
Equation
C06629 + C00005 + C00007 + C00080 <=> C06628 + C00006 + C00001
Comment
subsequently R05462
Reaction class
RC00001  C00005_C00006
RC00236  C06628_C06629
Enzyme
1.14.13.232
Pathway
rn00253  Tetracycline biosynthesis
rn01057  Biosynthesis of type II polyketide products
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00780  Tetracycline/oxytetracycline biosynthesis, pretetramide => tetracycline/oxytetracycline
M00823  Chlortetracycline biosynthesis, pretetramide => chlortetracycline
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.232
     R05463  6-Methylpretetramide + NADPH + Oxygen + H+ <=> 4-Hydroxy-6-methylpretetramide + NADP+ + H2O
Orthology
K14253  6-methylpretetramide 4-monooxygenase [EC:1.14.13.232]
Reference
1  [PMID:20522541]
  Authors
Pickens LB, Tang Y
  Title
Oxytetracycline biosynthesis.
  Journal
J Biol Chem 285:27509-15 (2010)
DOI:10.1074/jbc.R110.130419
Reference
2  [PMID:19472250]
  Authors
Wang P, Zhang W, Zhan J, Tang Y
  Title
Identification of OxyE as an ancillary oxygenase during tetracycline biosynthesis.
  Journal
Chembiochem 10:1544-50 (2009)
DOI:10.1002/cbic.200900122
Reference
3  [PMID:18422316]
  Authors
Zhang W, Watanabe K, Cai X, Jung ME, Tang Y, Zhan J
  Title
Identifying the minimal enzymes required for anhydrotetracycline biosynthesis.
  Journal
J Am Chem Soc 130:6068-9 (2008)
DOI:10.1021/ja800951e
Other DBs
RHEA: 50011
LinkDB

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