KEGG   REACTION: R05521
Entry
R05521                      Reaction                               
Name
erythromycin-B,NADPH:oxygen oxidoreductase (12-hydroxylating)
Definition
Erythromycin B + NADPH + H+ + Oxygen <=> Erythromycin + NADP+ + H2O
Equation
C06653 + C00005 + C00080 + C00007 <=> C01912 + C00006 + C00001
Reaction class
RC00001  C00005_C00006
RC01892  C01912_C06653
Enzyme
1.14.13.154
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.154
     R05521  Erythromycin B + NADPH + H+ + Oxygen <=> Erythromycin + NADP+ + H2O
Orthology
K14370  erythromycin 12 hydroxylase [EC:1.14.13.154]
Reference
1  [PMID:19625248]
  Authors
Savino C, Montemiglio LC, Sciara G, Miele AE, Kendrew SG, Jemth P, Gianni S, Vallone B
  Title
Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate.
  Journal
J Biol Chem 284:29170-9 (2009)
DOI:10.1074/jbc.M109.003590
Reference
2  [PMID:8416893]
  Authors
Stassi D, Donadio S, Staver MJ, Katz L
  Title
Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis.
  Journal
J Bacteriol 175:182-9 (1993)
DOI:10.1128/JB.175.1.182-189.1993
LinkDB

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