Escherichia coli BL21(DE3): ECD_01274 Help Entry ECD_01274         CDS       T00931                                  Symbol puuA Name (GenBank) glutamate--putrescine ligase   KO K09470   gamma-glutamylputrescine synthase [EC:6.3.1.11] Organism ebl  Escherichia coli BL21(DE3) Pathway ebl00330   Arginine and proline metabolism ebl01100   Metabolic pathways Module ebl_M00136   GABA biosynthesis, prokaryotes, putrescine => GABA Brite KEGG Orthology (KO) [BR:ebl00001]  09100 Metabolism   09105 Amino acid metabolism    00330 Arginine and proline metabolism     ECD_01274 (puuA) Enzymes [BR:ebl01000]  6. Ligases   6.3  Forming carbon-nitrogen bonds    6.3.1  Acid-D-ammonia (or amine) ligases (amide synthases)     6.3.1.11  glutamate---putrescine ligase      ECD_01274 (puuA) BRITE hierarchy SSDB OrthologParalogGene clusterGFIT Motif Pfam:  Gln-synt_C Gln-synt_N_2 FANCF Motif Other DBs NCBI-ProteinID:  ACT43162 LinkDB All DBs Position complement(1345856..1347274) Genome browser AA seq 472 aa AA seqDB search METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVAS LKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQM LLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRQRDAEGYLQPPCA PGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDN VLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDAE GEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPC GDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQDGLPFPIRQ SDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA NT seq 1419 nt NT seq  +upstreamnt  +downstreamnt atggaaaccaatatcgttgaagtagagaactttgttcagcagtcagaagagaggcggggt agcgcctttacgcaggaagtgaaacgctacctggagcgctacccgaatacgcaatatgtt gatgttttacttactgatttgaatggttgcttccgtggaaaacgtatccctgttgcaagc ctgaagaagttggagaaagggtgttatttcccggcctcggtgtttgcaatggatattctg ggcaacgtagtagaagaagcgggtctgggtcaggaaatgggcgagccggatcgtacttgt gttcctgttctcggttccttaaccccttctgccgccgatccagagtttatcggtcagatg ctcctgaccatggtcgatgaagatggcgctccctttgacgttgagccgcggaacgttctt aaccgcctgtggcagcagctacgccagcgcggattgttcccggttgtagcggtagagctg gagttctatttactggatcgtcagcgcgacgctgaagggtatctgcaaccgccctgcgcg cccggcaccgatgaccgcaatacacaaagccaggtttactccgttgataacctcaaccac ttcgccgacgtgctcaatgatattgatgaactggcgcagttacagttaattcccgccgac ggtgcggtcgccgaagcctcgccgggtcagtttgaaatcaacctttaccataccgataac gtgctggaagcctgcgatgatgcgctggcgctaaaacgcctggtgcgtctgatggcagaa aagcataagatgcacgccacttttatggcgaagccgtatgaagagcacgcgggcagcggg atgcatatccatatcagtatgcaaaataatcgtggcgagaacgtgctttctgacgcggaa ggcgaagattcgccgctgctgaaaaaaatgctcgccggaatgatcgatctgatgccgtca tcaatggcgttgctggcaccaaacgtgaactcgtatcgccgcttccagccggggatgtat gtaccgacgcaggcgtcgtggggccataacaaccgcaccgtcgccctgcgtattccatgc ggcgatcgtcataatcaccgcgtggaatatcgcgtggcgggtgccgatgccaacccatat ctggtgatggcagcgatttttgccggtattttgcacggtcttgataacgagctgccgttg caggaagaagtcgaaggcaacgggctggaacaggatggcttacccttcccgattcgccag agcgatgccctgggtgagtttatcgagaatgatcacctgcgccgctatttaggcgaacgg ttttgccatgtctatcacgcctgtaaaaatgatgagctgttgcagtttgagcgcctcatc acagaaacagaaattgagtggatgttgaaaaacgcgtaa

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