KEGG   ENZYME: 2.4.2.60
Entry
EC 2.4.2.60                 Enzyme                                 
Name
cysteine-dependent adenosine diphosphate thiazole synthase;
THI4 (gene name) (ambiguous);
THI1 (gene name);
ADP-thiazole synthase
Class
Transferases;
Glycosyltransferases;
Pentosyltransferases
Sysname
NAD+:glycine ADP-D-ribosyltransferase (dehydroalanine-producing)
Reaction(IUBMB)
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O [RN:R10685]
Reaction(KEGG)
R10685
Substrate
NAD+ [CPD:C00003];
glycine [CPD:C00037];
[ADP-thiazole synthase]-L-cysteine
Product
nicotinamide [CPD:C00153];
ADP-5-ethyl-4-methylthiazole-2-carboxylate [CPD:C20784];
[ADP-thiazole synthase]-dehydroalanine;
H2O [CPD:C00001]
Comment
This iron dependent enzyme, found in fungi, plants, and some archaea, is involved in the thiamine phosphate biosynthesis pathway. It is a single turn-over enzyme since the cysteine residue is not regenerated in vivo [3]. The homologous enzyme in archaea (EC 2.4.2.59, sulfide-dependent adenosine diphosphate thiazole synthase) uses sulfide as sulfur donor.
History
EC 2.4.2.60 created 2018
Pathway
ec00730  Thiamine metabolism
ec01100  Metabolic pathways
Orthology
K03146  cysteine-dependent adenosine diphosphate thiazole synthase
Genes
ATH: AT5G54770(THI1)
ALY: 9300430
CRB: 17876896
CSAT: 104726000 104731059 104763460
EUS: EUTSA_v10013978mg
BRP: 103844930 103851941 103857026
BNA: 106346007 106347311 106366413 106371241 106402237 106420919 125601255
BOE: 106318832 106327201 106335239
RSZ: 108836615 108837050 108844151 130501203
THJ: 104817652
CPAP: 110810965
CIT: 102626594(thi) 102629135
CIC: CICLE_v10008765mg CICLE_v10015727mg CICLE_v10024231mg
PVY: 116123427
MINC: 123202328 123225908
TCC: 18593644 18597097
GRA: 105769824 105773933 105780320
GHI: 107901923 107917921 107928645 107958248 107961959 121217764
GAB: 108453783 108482152 108486471
HSYR: 120126604 120147008 120160341 120179475 120189402 120197833 120209583
DZI: 111280819 111301627 111318798
EGR: 104450213
GMX: 100775614 100788953 100794288 547461(SC-04) 547462(SC-03)
GSJ: 114372051 114380540 114403613 114404912 114417056
PVU: PHAVU_007G052900g PHAVU_011G139100g
VRA: 106754377 106755182
VAR: 108325373 108339541
VUN: 114168645 114192234
VUM: 124843922 124843926
CCAJ: 109795024 109808173
APRC: 113872364
MTR: 11446281 25485243
TPRA: 123882686
CAM: 101491901 101507074 101511321 101512227
PSAT: 127097128
VVO: 131607286 131643448
LJA: Lj3g3v1010900.1(Lj3g3v1010900.1) Lj5g3v2060670.1(Lj5g3v2060670.1)
ADU: 107477997 107488645
AIP: 107630145 107643278
AHF: 112737808 112749911 112789941 112801227
LANG: 109331169 109332649 109348532 109351883
PCIN: 129286736 129298471 129298852 129315109
QSA: O6P43_011880 O6P43_028946 O6P43_031212
FVE: 101298688 101299651
RCN: 112168087 112201505 112201899
PPER: 18780719
PMUM: 103342510
PDUL: 117623858
MDM: 103429668 103448581
MSYL: 126589528 126615015
PXB: 103948770 103967326
ZJU: 107407137 107420469
MNT: 21395127
CSAV: 115717421
CSV: 101211553
CMO: 103501605
BHJ: 120091480
CMAX: 111483293 111487533
CMOS: 111438464 111448035
CPEP: 111783834 111785935 111788519
RCU: 8280736
JCU: 105632894
HBR: 110647144
MESC: 110601845 110611614
POP: 7470933 7479083 7485534
PEU: 105108187 105130169 105134848
PALZ: 118035853 118047445 118059587
JRE: 108983682 108993518 109022013
CILL: 122277821 122282819 122289841
CAVE: 132170520 132173535 132176015
QSU: 111989411 112016278
QLO: 115956034 115956545 115972735 115988494
TWL: 119988758 119988861
VVI: 100260728(THI1-1) 100265774(THI1-2)
VRI: 117909200 117923467
SLY: 101257192
SPEN: 107025837
SOT: 102598996
SSTN: 125856795
SDUL: 129892560
CANN: 107856341
LBB: 132620419
NTA: 107797059(ThiI) 107798781
NSY: 104224143
NTO: 104087540
NAU: 109232136
INI: 109167290 109187908
ITR: 116005701 116028414
SIND: 105167970 105175375
OEU: 111383503 111389379
EGT: 105948772 105962248
SSPL: 121740783 121742722 121743409 121757713 121775363 121800880
SMIL: 130985758
SHIS: 125188752 125202496
APAN: 127247867 127249630
HAN: 110890327 110912864
ECAD: 122591292 122611091
LSV: 111877285 111914386
CCAV: 112507403 112514948
DCR: 108199579 108204812 108211831
CSIN: 114275070 114297269
RVL: 131322345 131330436
AEW: 130751513 130751909 130754280 130789884
BVG: 104887771 104887775
SOE: 110795721
CQI: 110721030 110723750
ATRI: 130818300
MOF: 131145521
NNU: 104604883
MING: 122078253 122091022
TSS: 122640918
PSOM: 113279575 113284440 113287867 113288375
OSA: 4343443
DOSA: Os07t0529600-01(Os07g0529600)
OBR: 102709384
OGL: 127779938
BDI: 100841709
ATS: 109771029
TDC: 119281869 119330895 119338169
TAES: 123066078 123148177 123160697 123169109
TUA: 125525971
LPER: 127313554 127333271
LRD: 124669313 124679092 124689497 124695681
SBI: 8063504 8078187
ZMA: 542714 542715
SITA: 101752840 101774400
SVS: 117842602 117853273
PVIR: 120671321 120704075
PHAI: 112889419
PDA: 103716822 103721429 120107672
EGU: 105046898 105051965
MUS: 103975582 103991030
ZOF: 121967276 122021558 122025718 122052496
DCT: 110108175
PEQ: 110031149
AOF: 109827745 109834756
MSIN: 131221413 131239167
NCOL: 116249223
ATR: 18435056
SMO: SELMODRAFT_163429 SELMODRAFT_271002
PPP: 112273035 112273481 112275550 112276032 112276579
CRE: CHLRE_04g214150v5
VCN: VOLCADRAFT_74899 VOLCADRAFT_74900
MNG: MNEG_15292 MNEG_7985
CSL: COCSUDRAFT_14398
CVR: CHLNCDRAFT_22703
APRO: F751_1708
SCE: YGR144W(THI4)
SEUB: DI49_2107
SPAO: SPAR_G03600
AGO: AGOS_AAL137W
ERC: Ecym_6012
KLA: KLLA0_A00198g
KMX: KLMA_40625(THI4)
LTH: KLTH0B03916g
VPO: Kpol_1050p31
ZRO: ZYRO0G11726g
CGR: CAGL0M01166g
NCS: NCAS_0D01750(NCAS0D01750)
NDI: NDAI_0C02860(NDAI0C02860)
TPF: TPHA_0A04790(TPHA0A04790)
TBL: TBLA_0B09390(TBLA0B09390) TBLA_0H00760(TBLA0H00760)
TGB: HG536_0A04825 HG536_0F00230
TDL: TDEL_0H00200(TDEL0H00200)
KNG: KNAG_0E03150(KNAG0E03150)
ZMK: HG535_0B00510
PPA: PAS_chr3_0648
DHA: DEHA2F16654g
PIC: PICST_50942
PGU: PGUG_04211
SPAA: SPAPADRAFT_51398
LEL: PVL30_003709(THI4)
CAL: CAALFM_C305130CA(THI4)
CTP: CTRG_02525
COT: CORT_0C04770
CDU: CD36_85100
YLI: YALI0A09768g
CLU: CLUG_04547
CLUS: A9F13_11g02431
CAUR: CJI96_0001634
SLB: AWJ20_4223(THI4)
PKZ: C5L36_0C00170
BNN: FOA43_001955(STI35)
BBRX: BRETT_001261(THI4)
OPA: HPODL_03615
SLUD: SCDLUD_003155
NCR: NCU06110
NTE: NEUTE1DRAFT95662(NEUTE1DRAFT_95662)
SMP: SMAC_06409
PAN: PODANSg1687
PBEL: QC761_508470(THI4)
PPSD: QC762_508470(THI4)
PPSP: QC763_508470(THI4)
PPSA: QC764_508470(THI4)
TTT: THITE_2117402
MTM: MYCTH_2315910
CTHR: CTHT_0017160
MGR: MGG_03098
PPEI: PpBr36_09505
PGRI: PgNI_10244
TMN: UCRPA7_2510
SSCK: SPSK_07738
FGR: FGSG_02469
FPU: FPSE_01936
FPOA: FPOAC1_002586(STI35)
FVN: FVRRES_02879
FVR: FVEG_09077
FOX: FOXG_10428
NHE: NECHADRAFT_61890
FFC: NCS54_00810500
FKR: NCS57_00853700
FMU: J7337_005314(STI35)
TRE: TRIREDRAFT_68608(thi4)
TRR: M419DRAFT_89757
MAW: MAC_01186
MAJ: MAA_05549
PCHM: VFPPC_03188
CMT: CCM_01639
AMUS: LMH87_003448
PLJ: VFPFJ_09336
PTKZ: JDV02_002359(THI4)
VAL: VDBG_03908
VDA: VDAG_01137
CFJ: CFIO01_04759
CLUP: CLUP02_16278
CHIG: CH63R_12560
SAPO: SAPIO_CDS2560
ELA: UCREL1_6105
PFY: PFICI_09188
SSL: SS1G_09727
BFU: BCIN_07g06010(Bcthi4)
MBE: MBM_01288
PSCO: LY89DRAFT_683526
GLZ: GLAREA_02091
ANI: ANIA_03928(thiF)
AFM: AFUA_6G08360
ACT: ACLA_082310
NFI: NFIA_054010
AOR: AO090003000090
ANG: An11g01630
AFV: AFLA_001605
ALUC: AKAW2_80142S(THI4)
ACHE: ACHE_80625S(THI4)
APUU: APUU_40431S(THI4)
PCS: N7525_005441
PDP: PDIP_32520
POU: POX_d05776
TMF: EYB26_005937
TRG: TRUGW13939_06486
CIM: CIMG_01115
CPW: CPC735_052570
URE: UREG_01058
PBL: PAAG_01125
PBN: PADG_03708
ABE: ARB_07940
TVE: TRV_04098
AJE: HCAG_05915
BGH: BDBG_03342
PNO: SNOG_05965
PTE: PTT_08482
BZE: COCCADRAFT_27023
BSC: COCSADRAFT_38262
BOR: COCMIDRAFT_30031
AALT: CC77DRAFT_1021441
ARAB: EKO05_0000764
ZTR: MYCGRDRAFT_44262
PFJ: MYCFIDRAFT_211863
FFU: CLAFUR5_09695
CBET: CB0940_10496
BCOM: BAUCODRAFT_62660
NPA: UCRNP2_7340
SPO: SPBC26H8.01(thi2)
SOM: SOMG_00517(thi2)
TVS: TRAVEDRAFT_71517
DSQ: DICSQDRAFT_134894
PCO: PHACADRAFT_177254
SHS: STEHIDRAFT_111603
HIR: HETIRDRAFT_440039
PSQ: PUNSTDRAFT_42416
ADL: AURDEDRAFT_139075
FME: FOMMEDRAFT_23696
GTR: GLOTRDRAFT_77212
RSX: RhiXN_04607
CCI: CC1G_04976
ABP: AGABI1DRAFT40074(AGABI1DRAFT_40074)
ABV: AGABI2DRAFT72787(AGABI2DRAFT_72787)
PCUB: JR316_0006998
MRR: Moror_11852
MORE: E1B28_012388
SCM: SCHCO_02490971(SCHCODRAFT_02490971)
CPUT: CONPUDRAFT_104209
SLA: SERLADRAFT_470126
WSE: WALSEDRAFT_32181(CYPBP37)
WIC: J056_001533
UMA: UMAG_11400
PFP: PFL1_06351
SGRA: EX895_003350
MGL: MGL_1314
MSYM: MSY001_3139
PGR: PGTG_01304
PTRC: PtA15_7A55
PSTR: Pst134EA_013047
MLR: MELLADRAFT_53832
SMIN: v1.2.003820.t1(symbB.v1.2.003820.t1)
SPAR: SPRG_04526
GTT: GUITHDRAFT_93981
 » show all
Reference
1  [PMID:16912043]
  Authors
Godoi PH, Galhardo RS, Luche DD, Van Sluys MA, Menck CF, Oliva G
  Title
Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana.
  Journal
J Biol Chem 281:30957-66 (2006)
DOI:10.1074/jbc.M604469200
  Sequence
[ath:AT5G54770]
Reference
2  [PMID:22031445]
  Authors
Chatterjee A, Abeydeera ND, Bale S, Pai PJ, Dorrestein PC, Russell DH, Ealick SE, Begley TP
  Title
Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase.
  Journal
Nature 478:542-6 (2011)
DOI:10.1038/nature10503
  Sequence
[sce:YGR144W]
Reference
3  [PMID:26919468]
  Authors
Zhang X, Eser BE, Chanani PK, Begley TP, Ealick SE
  Title
Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole  Synthases.
  Journal
Biochemistry 55:1826-38 (2016)
DOI:10.1021/acs.biochem.6b00030
  Sequence
[sce:YGR144W]
Reference
4  [PMID:25348237]
  Authors
Hwang S, Cordova B, Chavarria N, Elbanna D, McHugh S, Rojas J, Pfeiffer F, Maupin-Furlow JA
  Title
Conserved active site cysteine residue of archaeal THI4 homolog is essential for thiamine biosynthesis in Haloferax volcanii.
  Journal
BMC Microbiol 14:260 (2014)
DOI:10.1186/s12866-014-0260-0
Other DBs
ExplorEnz - The Enzyme Database: 2.4.2.60
IUBMB Enzyme Nomenclature: 2.4.2.60
ExPASy - ENZYME nomenclature database: 2.4.2.60
BRENDA, the Enzyme Database: 2.4.2.60
LinkDB

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